摘要/Abstract
摘要： 目的 ·研究枸杞多糖（ Lycium barbarum polysaccharide，LBP）对髓样分化因子 88（myeloid differentiation factor 88，MyD88）基因敲除（ MyD88-/-）小鼠 2型糖尿病（ type 2 diabetes mellitus，T2DM）模型炎症因子的影响。方法 ·采用不同浓度 LBP（20、40、 80 mg/kg）干预 MyD88-/-T2DM小鼠， ELISA法检测小鼠血清中白细胞介素 1β（IL-1β）、IL-6、IL-8、转化生长因子 β1（TGF-β1）及 IL-10的水平。采用不同浓度（ 25、50、100 μg/mL）LBP预处理小鼠巨噬细胞系 Raw264.7细胞，然后用脂多糖诱导炎症状态，Western blotting检测各组细胞核因子 κB（NF-κB）的核转位变化，以及 NF-κB抑制蛋白（ IκB）和磷酸化 IκB的蛋白水平。结果 · LBP能够降低 MyD88-/-T2DM小鼠血清 IL-1β和 TGF-β1的水平（均 P<0.05）。体外实验表明， LBP可以剂量依赖性地抑制巨噬细胞中由脂多糖诱发的 NF-κB核转位，同时高剂量的 LBP可以抑制 IκB磷酸化。结论 · LBP可以抑制 MyD88-/-T2DM小鼠部分促炎症因子，这种调节作用可能与其弱化巨噬细胞 IκB磷酸化，抑制 NF-κB核转位有关。
关键词: 枸杞多糖, 髓样分化因子 88, 基因敲除, 2型糖尿病, 巨噬细胞, 炎症因子, 核因子 &, kappa, B
Abstract:
Objective · To investigate the effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus (T2DM) mice without myeloid differentiation factor 88 gene (MyD88-/-). Methods · Levels of interleukin 1β (IL-1β), IL-6, IL-8, transforming growth factor β1 (TGF-β1), and IL-10 in serum were assessedELISA in the MyD88-/-T2DM mice which had been administered with different doses of LBP (20, 40, and 80 mg/kg). Momacrophages Raw264.7 were stimulatedlipopolysaccharide (LPS) after treatment with different concentrations of LBP (25, 50, and 100 μg/mL). Then Western blotting was used to detect nuclear translocation level of nuclear factor κB (NF-κB) and protein s of inhibitor of NF-κB (IκB) and p-IκB. Results · Serum levels of IL-1β and TGF-β1 in MyD88-/-T2DM mice were down-regulatedLBP (P<0.05). Cell experiment proved that nuclear migration of NF-κB was dose-dependently inhibitedLBP, and the level of p-IκB was reducedhigh dose of LBP. Conclusion · LBP can reduce some proinflammatory cytokines in the MyD88-/-T2DM mice, which may be related with its inhibitive effect on the phosphorylation of IκB and nuclear migration of NF-κB in the macrophages.
Key words: Lycium barbarum polysaccharide (LBP), myeloid differentiation factor 88 (MyD88), gene knockout, type 2 diabetes mellitus (T2DM), macrophage, inflammatory cytokine, nuclear factor &, kappa, B (NF-&, kappa, B)
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