摘要/Abstract
摘要： 目的·探讨泛素连接酶E3C（ubiquitin protein ligase E3C，UBE3C）对卵巢癌SKOV3细胞增殖、侵袭能力的影响。方法· Western blotting法检测UBE3C蛋白在不同卵巢癌细胞株和正常卵巢上皮细胞中的表达。选择卵巢癌SKOV3细胞株转染si-UBE3C下调UBE3C表达，转染ex-UBE3C质粒上调UBE3C表达。CCK-8法、平板克隆实验检测细胞增殖能力的变化，细胞划痕、Transwell侵袭实验检测细胞迁移、侵袭能力的变化，并检测增殖、转移相关蛋白β-catenin、c-Myc的表达。结果· UBE3C在卵巢癌细胞株中的表达显著高于正常卵巢上皮细胞。si-UBE3C转染SKOV3细胞沉默UBE3C表达，细胞增殖能力下降，迁移及侵袭能力减弱，β-catenin、c-Myc蛋白表达下调。ex-UBE3C转染后UBE3C过表达，SKOV3细胞增殖、迁移、侵袭能力增强，且β-catenin、c-Myc蛋白表达水平升高。结论· UBE3C可能通过上调β-catenin及c-Myc蛋白表达，增强卵巢癌SKOV3细胞的增殖、侵袭能力。
关键词: 泛素连接酶E3（CUBE3C）, 卵巢癌, 细胞增殖, 迁移, 侵袭
Abstract:
Objective · To explore the effects of ubiquitin protein ligase E3C (UBE3C) on proliferation and invasion in epithelial ovarian cancer SKOV3cells. Methods · Western blotting was used to detect the difference of UBE3C in epithelial ovarian cancer cell lines and normal ovarian cell lines. SKOV3 cells were transfected with si-UBE3C to knockdown UBE3C protein level, while ex-UBE3C plasmid was used to upregulate the of UBE3C. Cell proliferation was measuredCCK-8 assay and colony formation assay. Wound healing assay and Transwell assay were performed to investigate the effect of UBE3C on migration and invasion. Protein levels of β-catenin and c-Myc were also detected in different groups, which were closely related to proliferation and invasion. Results · UBE3C was highly expressed in epithelial ovarian cancer cell lines. UBE3C was successfully silenced with si-UBE3C transfection in SKOV3 cells. Inhibition of UBE3C significantly weakened the abilities of cell proliferation, migration and invasion. A reduction of β-catenin and c-Myc protein levels was also accompaniedUBE3C knockdown. Over of UBE3C with exUBE3Cplasmid promoted the abilities of proliferation, migration and invasion. Enhanced levels of β-catenin and c-Myc were also verified. Conclusion · UBE3C promotes proliferation and invasion of epithelial ovarian cancer SKOV3 cells. This might be to do with upregulation of β-catenin and c-Myc protein levels.
Key words: ubiquitin protein ligase E3C (UBE3C), ovarian cancer, cell proliferation, migration, invasion
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