摘要/Abstract
摘要： 目的·研究系统性红斑狼疮（systemic lupus erythematosus，SLE）患者CD4+CD25+CD45RA-T细胞中转录因子BTB与CNC同源基因2（BTB and CNC homology 2, Bach2）的表达及其对细胞功能的影响。方法·采用流式细胞仪分选获得活动期SLE患者（SLE组）和健康对照者（对照组）外周血CD4+CD25+CD45RA-T细胞，荧光定量PCR和Western blotting检测该类细胞中Bach2的表达；分析SLE患者该类细胞中Bach2的平均荧光强度（median fluorescence intensity，MFI）与SLE疾病活动指数（systemic lupus erythematosus disease activity index，SLEDAI）的相关性。采用流式细胞术比较SLE患者IL-17+CD4+CD25+CD45RA-T细胞和IL-17CD4+CD25+CD45RA-T细胞中Bach2的MFI。在Bach2过表达体系中，采用流式细胞术检测CD4+CD25+CD45RA-T细胞表达IL-17的细胞比例，采用ELISA方法检测细胞培养上清液中IL-17的含量。结果·与对照组相比，SLE组CD4+CD25+CD45RA-T细胞Bach2的mRNA和蛋白表达水平均显著降低（均P<0.01）；SLE组Bach2的MFI与SLEDAI呈显著负相关（R20.433，P0.001）。SLE患者IL-17+CD4+CD25+CD45RA-T细胞Bach2的MFI显著低于IL-17-CD4+CD25+CD45RA-T细胞（P0.013）。当Bach2过表达时，SLE患者CD4+CD25+CD45RA-T细胞表达炎症因子IL-17的细胞比例明显下降（P0.032），细胞培养上清液中IL-17含量显著降低（P0.008）。结论· SLE患者CD4+CD25+CD45RA-T细胞Bach2表达下降，在该类细胞中过表达Bach2可使IL-17表达下降。
关键词: 系统性红斑狼疮, CD4+CD25+CD45RA-T细胞, BTB与CNC同源基因2, 白细胞介素17
Abstract:
Objective · To study the of transcription factor BTB and CNC homology 2 (Bach2) in CD4+CD25+CD45RA-T cells patients with systemic lupus erythematosus (SLE) and its effect on cell function. Methods · The CD4+CD25+CD45RA-T cells active SLE patients and healthy volunteers were sortedflow cytometry. The of Bach2 in CD4+CD25+CD45RA-T cells was detectedfluorescence quantitative PCR and Western blotting. The correlation between the median flourscence indensity (MFI) of Bach2 in CD4+CD25+CD45RA- T cells and the disease activity index of SLE (SLEDAI) was analyzed. The MFI of Bach2 in IL-17+CD4+CD25+CD45RA-T cells was compared with that inIL-17-CD4+CD25+CD45RA-T cellsflow cytometry. In Bach2 over system, the of IL-17 in CD4+CD25+CD45RA- T cells was detectedflow cytometry and the concentration of IL-17 in the culture supernants was detectedELISA. Results · The mRNA and protein s of Bach2 in CD4+CD25+CD45RA-T cells SLE patients were significantly lower than those in healthy controls (P<0.01). There was a significant negative correlation between the MFI of Bach2 and SLEDAI (R20.433, P0.001) in patients with SLE. The of Bach2 in IL17+CD4+CD25+CD45RA-T cells was significantly lower than that in IL-17-CD4+CD25+CD45RA-T cells (P0.013). When Bach2 was overexpressed, the percentage of CD4+CD25+CD45RA-T cells SLE patients expressing inflammatory factor IL-17 decreased significantly (P0.032) and the IL-17concentration in cell culture supernatants markedly decreased (P0.008). Conclusion · The of Bach2 in CD4+CD25+CD45RA-T cells SLE patients decreases, and over of Bach2 in the cells leads to the falling of IL-17.
Key words: systemic lupus erythematosus, CD4+CD25+CD45RA-T cell, BTB and CNC homology 2 (Bach2), interleukin-17
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