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补骨脂素和异补骨脂素对人牙周膜细胞的抗炎作用

本站小编 Free考研考试/2022-02-12

摘要/Abstract


摘要: 目的 · 观察补骨脂素和异补骨脂素对人牙周膜细胞炎症因子表达的影响。方法 · 组织块法原代培养人牙周膜细胞。CCK-8 试验检测 2 种药物对人牙周膜细胞活性的影响。用不同浓度的补骨脂素和异补骨脂素预处理人牙周膜细胞 2 h 后,再用牙龈卟啉单胞菌脂多糖(Porphyromonas gingivalis lipopolysaccharide,Pg-LPS)刺激,real-time PCR 和 ELISA 分别检测白介素 -1β(interleukin-1β,IL-1β)和白介素 -8(IL-8)的 mRNA 和蛋白表达。结果 · 采用组织块法可成功培养出人牙周膜细胞。12.5 μg/mL 及以下浓度的补骨脂素和异补骨脂素对人牙周膜细胞活性无影响。补骨脂素和异补骨脂素不仅可下调 Pg-LPS 诱导的人牙周膜细胞 IL-1β 和 IL-8 mRNA 的表达,还可抑制 IL-1β 和 IL-8 蛋白的分泌。结论 · 补骨脂素和异补骨脂素可显著抑制 Pg-LPS 诱导的人牙周膜细胞炎症因子的表达,可用于牙周炎的治疗和预防。
关键词: 人牙周膜细胞, 补骨脂素, 异补骨脂素, 抗炎
Abstract:
Objective · To investigate the effects of psoralen and angelicin on inflammation cytokine expression of human periodontal ligament cells (hPDLCs). Methods · hPDLCs were primarily cultured using tissue explant method. Effects of psoralen and angelicin on the cell viability were tested by CCK-8 assay. hPDLCs were stimulated by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) after treatment with different concentrations of psoralen and angelicin for 2 h. mRNA expression of IL-1β and IL-8 were determined by real-time PCR. Enzyme-linked immunosorbent assay (ELISA) was used to measure the secretion of IL-1β and IL-8. Results · hPDLCs were cultured successfully by tissue explant method. Psoralen and angelicin (≤ 12.5 μg/mL) did not show significant effects on the cell viability of hPDLCs. Pg-LPS markedly elevated the mRNA expression of IL-1β and IL-8,which could be attenuated by psoralen and angelicin in a dose-dependent manner. Likewise, the up-regulated protein secretion of IL-1β and IL-8 could be significantly blocked by psoralen and angelicin. Conclusion · Psoralen and angelicin could attenuate the inflammatory response of hPDLCs induced by Pg-LPS. Therefore, psoralen and angelicin may act as natural agents to prevent and treat periodontitis.

Key words: human periodontal ligament cells, psoralen, angelicin, anti-inflammation


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