KCJJ"免疫学技术在生命科学各研究领域，包括基础和临床医学各学科中均有广泛运用。本课程通过理论课与实验课有机结合的形式，介绍免疫学技术的概况，基本理论、基本技术和基本操作。理论课共有三次，授课内容包括：一、概述；二、抗原抗体制备：1.抗原制备，2.抗体制备（多克隆抗体、单克隆抗体、基因工程抗体），3.抗体标记；三、抗原抗体检测技术：1.血清学方法，2.免疫标记技术（同位素、酶、荧光素、镧系元素螯合物、化学发光、金属）；四免疫状态检测：1.免疫细胞分离、分析与培养，2.表面标志检测和免疫细胞亚群分析3.细胞因子检测，4.免疫细胞功能检测5.凋亡研究，6.实验动物模型。通过理论的学习使学生对免疫学技术所包含的内容、应用范围、技术要点、注意事项等有一个概括的了解。实验课共有七次，通过实验课的实际操作，希望使学生对各类免疫学技术的操作过程有一个感性的认识。实验一、免疫球蛋白的提取与纯化实验二、免疫球蛋白的鉴定（免疫双扩、免疫电泳）实验三、荧光抗体的标记、纯化与鉴定实验四、ELISA实验五、外周血单个核细胞的分离与鉴定实验六、淋巴细胞表面抗原检测与亚群分析（FACs法）实验七、微量淋巴细胞毒试验本课程所要达到的目的是：使学生在今后的科研工作中，知道有那些免疫学技术可以作为自己课题研究的有效工具，如何通过查阅有关书籍和文献资料去完善自己的研究思路和技术路线，并设计出切实可行的实验方法。"

KCJJY"Immunological techniques have broad applications in the field of life science research including basic and clinical medicine. The courses introduce immunological techniques with profiles, basic theory, basic skills and basic operation through theories and experiments in the form of a combination of classes. There are three classes about Immunotechnology including: part I, outlined; part II, preparation of antigen and antibody: 1. antigen preparation, 2. antibody preparation (polyclonal antibody, monoclonal antibody, genetic engineering antibody), 3. antibody labeling; part III, techniques based on antigen-antibody interactions: 1. serum method 2. immunolabeling techniques (isotopes, enzymes, fluorescence, lanthanum, chemiluminescence, metal); part IV, analysis of immune function : 1. separation, analysis and culture of immune cells, 2. detection of CD molecules and analysis of immune cell subgroups, 3. assay of cytokine, 4. immune cell function testing, 5. detection of cell apoptosis, 6.experimental animal models.Learning of these classes make students get a general understanding for immunological techniques such as content, application, technical elements, matters needing attention .A total of seven experimental classes provide practical opportunities of operation that enable students to experience the technical operation course:Experiment I, extraction and purification of immunoglobulinExperiment II, identification of immunoglobulin(double gel diffusion, immunoelectrophoresis)Experiment III, preparation, purification and identification of fluorescein labeled antibodiesExperiment IV, ELISAExperiment V, separation and identification of peripheral mononuclear blood cellExperiment VI, detection of lymphocyte surface antigen, analysis of immune cell subgroups (FACs method)Experiment VII, complement dependent cytotoxicity test"