分子生物学技术是现代分子生物医学研究中的重要研究工具。本课程是为即将进入这个领域的研究生提供一个实验学习的平台。分子生物学实验主要有三个部分组成：它们分别是基因组DNA分析、基因表达分析（包括RNA水平、蛋白质水平）、基因工程技术。主要实验由高分子量的DNA 提取和纯化、总RNA的提取和纯化（Trizol方法）、限制性内切酶的应用、DNA印迹分析（琼脂糖凝胶电泳分离DNA片段、DNA转移、核酸分子杂交技术）、探针的标记、逆转录聚合酶链反应、聚丙烯酰胺凝胶等电聚焦电泳、蛋白印迹分析、重组DNA 分子构建的策略、重组分子导入受体细胞、重组分子的筛选和鉴定等。另外我们还将介绍一些分子生物学研究领域中的一些其它技术，如蛋白质N-末端氨基酸的序列检测（DABITC双偶合法）、原位杂交技术在研究中的应用、实时定量-PCR，2D-蛋白电泳等，通过本课程的学习，使学生能掌握基本的分子生物学实验原理和方法，为他们以后的研究工作打下实验基础。

Techniques of molecular biology are very important tools for modern molecular biomedical research. For providing students who had little experience in molecular biological experiments with a studying platform, we launched this course. The whole course comprises three parts: analysis of genomics DNA, analysis of gene expression at RNA and protein level and genetic engineering. The content of the course includes extraction and purification of high molecular weight eukaryotic DNA, extraction of RNA from tissue, restrictive enzyme digestion, Southern blot, probe labeling, reverse transcription-PCR, Western blot, iso-electric focus gel electrophoresis, Techniques of Genetic Engineering (including construction of recombinant DNA and transformation to Ecoli., selection and identification of recombinant DNA) and so on. In addition, we will introduce some other techniques, such as analysis of protein N-terminal sequence, in situ hybridization, Real-Time-PCR, 2D-electrophoresis, etc. After the course is finished, the students will grasp the principles and methods of molecular biological experiments and establish foundation of their future research careers.