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Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region_上海

上海光学精密机械研究所 免费考研网/2018-05-06

外文题目: Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region
作者: Bai, Yeran; Zhang, Delong; Li, Chen; Liu, Cheng; Cheng, Ji-Xin
刊名: J. Phys. Chem. B
年: 2017 卷: 121 期: 44 页: 10249--10255
英文关键词:

DIFFERENCE-FREQUENCY-GENERATION; STIMULATED RAMAN-SCATTERING; QUANTUM CASCADE LASER; INFRARED-SPECTROSCOPY; MU-M; SPATIAL-RESOLUTION; LIPID DROPLETS; ABSORPTION; PULSES
英文摘要:
Using a visible beam to probe the thermal effect induced by infrared absorption, mid-infrared photothermal (MIP) microscopy allows bond-selective chemical imaging at submicron spatial resolution. Current MIP microscopes cannot reach the high wavenumber region due to the limited tunability of the existing quantum cascade laser source. We extend the spectral range of MIP microscopy by difference frequency generation (DFG) from two chirped femtosecond pulses. Flexible wavelength tuning in both C-D and C-H regions was achieved with mid-infrared power up to 22.1 mW and spectral width of 29.3 cm(-1). Distribution of fatty acid in live human lung cancer cells was revealed by MIP imaging of the C-D bond at 2192 cm(-1).


文献类型: 期刊论文
正文语种: English
收录类别: SCI
DOI: 10.1021/acs.jpcb.7b09570


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