外文题目: Wavefront-sensing-based autofocusing in microscopy
作者: Xu, Jing; Tian, Xiaolin; Meng, Xin; Kong, Yan; Gao, Shumei; Cui, Haoyang; Liu, Fei; Xue, Liang; Liu, Cheng; Wang, Shouyu
刊名: J. Biomed. Opt.
年: 2017 卷: 22 期: 8 文章编号：86012
英文关键词:
autofocusing; wavefront sensing; microscopy
INTENSITY EQUATION METHOD; DIGITAL HOLOGRAPHY; ADAPTIVE OPTICS; REAL-TIME; PHASE; TRANSPORT; AMPLITUDE; TRACKING; FOCUS
英文摘要:
Massive image acquisition is required along the optical axis in the classical image-analysis-based autofocus method, which significantly decreases autofocus efficiency. A wavefront-sensing-based autofocus technique is proposed to increase the speed of autofocusing and obtain high localization accuracy. Intensities at different planes along the optical axis can be computed numerically after extracting the wavefront at defocus position with the help of the transport-of-intensity equation method. According to the focus criterion, the focal plane can then be determined, and after sample shifting to this plane, the in-focus image can be recorded. The proposed approach allows for fast, precise focus detection with fewer image acquisitions compared to classical image-analysis-based autofocus techniques, and it can be applied in commercial microscopes only with an extra illumination filter. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.


文献类型: 期刊论文
正文语种: English
收录类别: SCI
DOI: 10.1117/1.JBO.22.8.086012


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