外文题目: The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers
作者: Wang, Jing; Wang, Xiongwei; Wang, Shimiao; Huang, Zheng; Liu, Jun
刊名: Laser Phys. Lett.
年: 2016 卷: 13 期: 9 文章编号：95602
英文关键词:
carbon nanodots; photodynamic therapy; chlorin e6
DRUG-DELIVERY; NANOPARTICLES; RELEASE; PHOTOSENSITIZERS; LUMINESCENCE; NANOTUBES; PARADIGM; NANORODS; CARRIERS; DOTS
英文摘要:
Chlorin e6 (Ce6), a large heterocyclic aromatic molecule, is a promising photosensitizer for photodynamic therapy (PDT). We propose an efficient nano-photosensitizer delivery system based on covalent interactions between Ce6 and polyethylenimine (PEI) coated carbon nanodots (CDots). We observed >50% Ce6 drug loading content for PEI, due to this compound's unique 'proton sponge effect.' We found that the covalently incorporated Ce6 molecules retained their functional properties for near-infrared (NIR) fluorescence imaging and PDT. The chemical characteristics of CDot-PEI-Ce6 and Ce6 were evaluated using different analytical methods, including transmission electron microscopy and UV-Visible absorption spectra. Time-correlated single photon counting (TCSPC) and fluorescence spectra were used to demonstrate that Ce6 successfully conjugated to the CDots. The high cellular uptake of CDots-PEI-Ce6 was confirmed using flow cytometry and confocal laser scanning microscopy. According to the MTT assay, the CDots-PEI-Ce6 exhibited low dark toxicity and efficient PDT efficacy to HeLa cancer cells. These results indicate that CDot-PEI-Ce6 conjugates are potential photosensitizer delivery systems for PDT.


文献类型: 期刊论文
正文语种: English
收录类别: SCI
DOI: 10.1088/1612-2011/13/9/095602


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