外文题目: A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging
作者: Chi, Shuheng; Li, Liang; Wu, Yiqun
刊名: Sens. Actuator B-Chem.
年: 2016 卷: 231 页： 811--829
英文关键词:
Dibenzothiophene-based labels; Two-photon fluorescent probes; Nuclear localization; Biological imaging
LIVING CELLS; 2-PHOTON-ABSORBING CHROMOPHORES; OPTICAL-PROPERTIES; CROSS-SECTIONS; QUANTUM DOTS; DNA; PH; TISSUES; NANOPARTICLES; MITOCHONDRIA
英文摘要:
In this study, a series of novel dibenzothiophene-based two-photon excited fluorescence (TPEF) probes with excellent nuclear staining ability were prepared and investigated. The one-photon photophysical properties indicated these labels possessed UV absorption wavelength of 390-393 nm and quantum yields of 0.425-0.71. The two-photon photophysical performance exhibited two-photon absorption (TPA) cross-sections of 506-630 GM by excitation of femtosecond laser at a wavelength of 800 nm. 18-30-fold one-photon excited fluorescence (OPEF) enhancement and 300-450% TPEF increment binding with calf thymus DNA in Tris-HC1 buffer solutions displayed obvious fluorescence "turn-on" effect. The co-localization studies showed mean co-localization coefficients of 0.88-0.94 by double-staining with commercial nuclear staining reagent DAPI in 3T3 cells, indicating accurate nuclear localization ability. The confocal fluorescence imaging displayed clear TPEF nuclear imaging. The staining penetration depth of 0-9 mu m exhibited good membrane permeability and three dimensional imaging ability. The observation time of 1800 s and fluorescence decay amplitude of 8-10% within 300 s under persistent irradiation were achieved in TPEF imaging for nuclear labeling. The DNA binding mechanism was discussed as well. (C) 2016 Elsevier B.V. All rights reserved.


文献类型: 期刊论文
正文语种: English
收录类别: SCIEI
DOI: 10.1016/j.snb.2016.03.006


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