外文题目: Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging
作者: Chi, Shuheng; Li, Liang; Wu, Yiqun
刊名: J. Phys. Chem. C
年: 2016 卷: 120 期: 25 页： 13706--13715
英文关键词:

LIVING CELLS; DNA; COMPLEXES; SINGLE; RNA; MITOCHONDRIA; COMPARTMENTS; EXCITATION; MOLECULES; PHOTON
英文摘要:
A series of fluorenone-based two-photon fluorescent probes with high photostability for nucleus imaging are prepared and developed. The one- and two-photon photophysical properties exhibit these new probes possess 0.448-0.634 of fluorescence quantum yields and 469-495 GM of two-photon absorption cross sections at 800 nm femtosecond laser excitation. The luminescence "turn-on" experiment in buffer solutions indicates that 35-fold of fluorescence intensity and 68-fold fluorescence quantum yield enhancement appear between new probes and calf thymus DNA. In the nuclear double staining experiment, the high mean colocalization coefficients of 0.92-0.96 between new probes and nuclear labeling dye Hoechst 33342 are acquired, demonstrating excellent nuclear localization in 3T3 cells. The counterstain studies by introducing commercial mitochondrial staining reagent MTR and nuclear staining dye DAPI further show good membrane permeability and counterstain compatibility in multicolor cell labeling application. The photostability studies show that 3000 s of observation time and 0.028%/s-0.03%/s of mean fluorescence attenuation rates under persistent laser irradiation in two-photon nuclear imaging are achieved. Meanwhile, the cause of fluorescence attenuation in the photostability test for cellular nuclei monitoring are discussed as well.


文献类型: 期刊论文
正文语种: English
收录类别: SCIEI
DOI: 10.1021/acs.jpcc.6b02827


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