摘要/Abstract

近年来，使用微纳米制造工艺将蛋白质或多肽进行高精度空间图案化，推动了细胞生物学、组织工程学、药物科学等领域的发展.同时，羊毛角蛋白作为一种储量大的天然生物蛋白质，具有优异的水溶性、良好的生物相容性和可控的降解性，但羊毛角蛋白通常不能自组装形成凝胶网络或其他不溶形式，因此，使用羊毛角蛋白制备如纤维、薄膜、凝胶等的成型结构存在很大困难.本工作通过使用化学修饰的方法，在角蛋白上接枝功能基团，使角蛋白获得光敏感性，探究了共价交联法制备具有表面微结构角蛋白膜的可行性.并用3D激光扫描显微镜、紫外可见近红外光谱仪和傅里叶变换显微红外光谱仪对薄膜结构进行了表征.结果表明，使用软光刻法可以得到表面微结构完整度很高的角蛋白膜.本工作对羊毛角蛋白共价交联法进行了实验探索，实验结果不仅为人们提供了一种软光刻技术制备具有表面微结构的角蛋白膜的方法，而且为羊毛角蛋白制备成型结构提供了新的途径.
关键词: 羊毛角蛋白, 软光刻, 共价交联, 图案化, 表面微结构
Recently, the use of micro-nano manufacturing processes to fabricate high-precision spatial patterns of proteins or peptides has provided important applications in cell biology, tissue engineering, pharmaceutical science, and optoelectronics. As a natural biological protein, wool keratin (WK) have excellent water solubility, good biocompatibility, and controllable degradability. However, WK usually cannot self-assemble to form a gel network or other insoluble forms. Therefore, it is difficult to prepare molded WK materials, such as a fiber, a film, and a gel. To solve this problem, this paper explores the feasibility of preparing photocrosslinkable WK. WK was extracted from wool fibres, and its side groups were reacted with the reagent 2-isocyanatoethyl methacrylate (IEM), yielding a photoactive WK precursor. And then, WK films with patterned microstructures were obtained by a covalent cross-linking method. This method can also be used to obtain other forms of WK materials. The as-prepared WK films were characterized by 3D laser scanning microscopy, UV-visible near-infrared spectroscopy and Fourier transform infrared microscopy. The experimental results showed that after two pattern shifts, the pattern on the WK film still maintained good integrity and conformed to the original pattern on the silicon wafer, which indicated that the pattern transfer method can achieve perfect reproduction of the pattern. In addition, we also demonstrated that the formation of structural colors caused by periodically arranged microstructures on WK films. Our experimental results not only provide a facile method to prepare WK films with surface microstructures by soft lithography but also give a new way for the preparation of molded WK. We expect the good optical properties and controlled degradation properties of WK open up new directions for the manufacture of biodegradable optics and implantable flexible microelectronic devices.
Key words: wool keratin, soft lithography, covalent cross-linking, patterns, surface microstructure


PDF全文下载地址:

点我下载PDF