摘要/Abstract

对蜜环菌YUD17010发酵提取物进行化学研究, 从中分离纯化得到三个原伊鲁烷型倍半萜芳基酯, 包括一个新化合物armilaroma (1)以及两个已知化合物4-dehydrodihydromelleolide (2)和armillarivin (3). 通过波谱数据分析确定了它们的结构和相对立体构型, 并借助基于量子化学计算的电子圆二色性(ECD)谱确定了化合物的绝对构型. Armilaroma (1)表现出中等的乙酰胆碱酯酶(AChE)抑制活性, IC₅₀值为9.03 μmol/L. 此外, 还评估了化合物1对人类癌症细胞MCF-7、SW480、HL-60、A-549和SMMC-7721的细胞毒活性, IC₅₀值范围为13.73~17.92 μmol/L.
关键词: 蜜环菌, 真菌代谢产物, 细胞毒活性, 倍半萜芳基酯
A chemical investigation on the cultures extract of the basidiomycete Armillaria sp. YUD17010 led to the isolation of three protoilludane-type sesquiterpene aryl esters, including a new one armilaroma (1), along with two known ones 4-dehydrodihydromelleolide (2) and armillarivin (3). Their structures and relative configurations were established on the basis of spectroscopic data analysis, and the absolute configurations were assigned by electronic circular dichroism (ECD) spectra aided by quantum chemical calculations. Armilaroma (1) exhibited moderate acetylcholinesterase (AChE) inhibitory activity with an IC₅₀ value of 9.03 μmol/L. Moreover, cytotoxicities against the MCF-7, SW480, HL-60, A-549, and SMMC-7721 human cancer cell lines were also evaluated for compound 1, with the IC₅₀ values ranging from 13.73 μmol/L to 17.92 μmol/L.
Key words: Armillaria sp., fungal metabolite, cytotoxicity, sesquiterpene aryl ester


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