摘要/Abstract

以1，4-二乙基-7-羟基四氢喹喔啉-6-甲醛为基础，设计合成了一种具有长波长发射、较大斯托克斯位移的新型肼二腙类荧光探针L.在探针L的二甲基亚砜（DMSO）溶液中加入Cu²⁺和Co²⁺后，溶液颜色由黄色分别变为粉色和无色，可裸眼识别Cu²⁺和Co²⁺.利用L与Cu²⁺形成的配合物，在DMSO/H₂O（V∶V=7∶3，HEPES，1×10^-2 mol/L，pH=7.4）缓冲溶液中可荧光“OFF-ON”识别H₂S.试纸条实验表明，L-Cu²⁺对不同浓度H₂S显色明显，可以通过裸眼和荧光双通道快速检测H₂S.此外，利用Discovery Studio分子模拟软件，通过分子L结构反向找靶，并用CDOCKER程序进行分子对接，发现分子L对2x0v蛋白（来自人类的P53蛋白）有很好的结合作用，有望在医药领域得到应用.
关键词: 长波长发射, 荧光探针, H₂S, 识别, 分子对接
A novel fluorescent probe L with long wavelength emission and large Stokes shift was designed and synthesized based on 1,4-diethyl-7-hydroxytetrahydroquinoxaline-6-carboxaldehyde. The color of L was changed from yellow to pink and colorless upon addition of Cu²⁺ and Co²⁺ respectively in dimethylsulfoxide (DMSO), which illustrated that probe L could recognize Cu²⁺ and Co²⁺ by naked eyes. The complex L-Cu²⁺ could recognize H₂S with fluorescence "OFF-ON" in DMSO/H₂O (V:V=7:3, HEPES, 1×10^-2 mol/L, pH=7.4). The test strip assay showed that the L-Cu²⁺ pretreated test strip showed obvious color changes toward different concentrations of H₂S, which indicated that H₂S could be rapidly detected by naked eyes and fluorescent double channel. In addition, the molecular target of compound L can be searched by utilizing the reverse docking method in Discovery Studio (DS) software. Then, docking analysis was performed using CDOCKER module of DS, and it was found that molecule L has a good combination to 2x0v protein (P53_human). Therefore, it is expected to be applied in the medicine field.
Key words: long wavelength emission, fluorescent probe, H₂S, recognization, molecular docking


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