复旦大学人类新基因研究室



复旦大学遗传工程国家重点实验室人类新基因研究室是在著名遗传学家谈家桢院士和赵寿元教授的直接指导下，由余龙博士于1991年负责创建，其间先后承担了国家“863”高科技计划、“八五”、“九五”和“十五”期间重点和重大项目子课题，国家“973”计划重点项目子课题，国家自然科学基金课题，国家杰出青年基金课题以及上海市科委的重点和重大课题等19个项目。先后在Biochemical Journal、Genomics和中国科学等期刊上发表SCI论文50篇，EI论文54篇。申报人类新基因的国家发明专利105项，国际专利6项,获得美国专利3项。

该实验室作为国家人类基因组南方研究中心的卫星实验室之一，近年来的工作主要集中 ○1人类新基因的分离、克隆与功能研究； ○2肝癌发生、转移的分子机制以及诊断、治疗的研究。在已克隆的410个人类新基因中，已鉴定出69个与肝癌相关基因和4个细胞因子基因。这些新基因以及基因产物的深入研究与开发研究，有着潜在的巨大经济价值。

该实验室依靠复旦大学多学科的综合优势，有着从事生物学研究得天独厚的条件，吸引了一批又一批有志于人类基因研究的青年加盟，目前在实验室工作的人员达60人,其中工作人员 12人（教授2人、副教授2人） ，博士后3人，博士生32人，硕士生4人，高级访问学者3人。实验室成立以来，已培养、联合培养毕业（出站）博士后5人，博士生17人，硕士生15人。

由于实验室的工作成绩突出，2000年经国家自然科学基金委员会生命科学部专业评审组推荐， 10月国家自然科学基金委员会陈佳洱主任率专家亲临该室进行实地考核。而后于2000年12月正式被遴选为“创新研究群体”，获得全国首批六个试点“创新研究群体科学基金”的资助。

学术负责人：余龙，男，48岁，医学博士，遗传学教授，国家教育部“长江奖励计划”特聘教授，现已担任复旦大学遗传工程国家重点实验室常务主任，兼国家人类基因组南方研究中心副主任，中国遗传学会基因组学委员会副主任，中华医学遗传学、国外医学医学遗传学分册、中华新医学、中华中西医等杂志编委。他先后获得中国优秀博士后奖，上海市模范军队转业干部奖，香港求是科技基金会“杰出青年学者奖”，上海市科技精英提名奖，上海市优秀青年教师和宝钢教育基金会优秀教师奖，福建省科技进步二等奖等部省级科技进步奖5项。其事迹也被中央电视台的“东方之子”栏目播出。





[发表论文] (2000-2002)
以联系作者发表的SCI论文
1. Lihua ZHENG, Long Yu,Qiang Tu ,Min ZHANG,Hua HE,Weijie CHE,Jie GAO, Jianqiang YU ,Qianqhong WU and Shouyyuan ZHAO.Cloning and mapping of PKIB and PKIG ,and comparison of tissue expression pattern of three members of the protein kinase inhibitior family ,including PKIA. Biochemical Journal.2000,349(2):403-407. (IF=4.326)
2. Qiang TU, Long YU, Pingzhao ZHANG, Min ZHANG, Honglai ZHANG, Juxiang JIANG, Chiyuan CHEN, Shouyuan ZHAO. Cloning, characterization and mapping of the human ATP5E gene, identification of pseudogen ATP5EP1, and definition of the ATP5E motif. Biochemical Journal .2000,347:17-21. (IF=4.326)
3. He H, Dai F, Yu L, She X, Zhao Y, Jiang J, Chen X, Zhao S.Identification and characterization of nine novel human small GTPases showing variable expressions in liver cancer tissues. Gene Expr 2002;10(5-6):231-42. (IF=3.536)
4. Qiang Fu, Long Yu, Qing Liu, Jianxuan Zhang, Honglai Zhang, Shouyuan Zhao. Molecular Cloning, Expression Characterizaion and Mapping of a novel putative inhibitor of Rho GTPase activity, RTKN, to human chromosome 2p13. Genomics. 2000,66:328-332. (IF=3.418)
5. Yong Zhao Long Yu, Qiang Fu,Weijie Chen Jianming Jiang Jie Gao,and Shouyuan Zhao. Cloning and charaterization of human DDX24 and mouse Ddx24, two novel putative DEAD-Box proteins, and maping DDX24 to human chromosome 14q32.2000.67:351-355. Genomics. (IF=3.418)
6. Peirong Hu, Long Yu ,Min Zhang,Lihua Zheng,Yong Zhao,Qiang Fu,andshouyuan Zhao. Molecuar cloning and mapping of the barin-abundant B1γ subunit of protein phosphatase 2A,PPP2R2C,to human chromosome 4p16. Genomics..2000 .67:83-86. (IF=3.418)
7. Tieming Lang, Long Yu, Qiang Tu, Jianming Jiang, Zheng Chen, Yurong Xin, Guoyang Liu, Shouyuan Zhao. Molecular Cloning, Genomic organization and Mapping of PRKAG2, a novel heart abundant γ2 Subunit of 5’-AMP-activated Protein Kinase, to Human Chromosome 7q32.2. Genomics.2000.70:258-263 (IF=3.418)
8. Pingzhao Zhang, Long Yu,Jie Gao,Qiang Fu,Fangyan Dai, Yong Zhao,Lu Zheng,Shouyuan Zhao.Cloning and characterization of human VPS35 and muuse Vps35 Genomics.2000.70:253-257 (IF=3.418)
9. Jie Gao , Long Yu ,Pingzhao Zhang, Jianming Jiang,Jian Chen,Jisong Peng,Youheng Wei,and Shouyuan Zhao.Clong and charaterization of human mouse mitochondrial elongation factor G,GFM and Gfm,and mapping of GFM to human chromosome 3q25.1-q26.2 Genomics 2001,74(1):109-114. (IF=3.418)
10. Yurong Xin, Yu L, Zheng Chen, Lihua Zheng, Qiang Fu, Jianmin Jiang, Pingzhao Zhang, Romu Gong, Shouyuan Zhao. Cloning ,expression patterns and localization of three human and two mouse homologues of GABAA Receptor-associated protein Genomics 2001,74:408-413 (IF=3.418)
11. Zhang J, Yu L, Fu Q, Gao J, Xie Y, Chen J,Zhang P, Liu Q, Zhao S .Mouse phosphoglycerate mutase M and B isozymes:cDNA cloning ,enzyme activity assay and mapping. Gene 2001,264(2):273-279. (IF=3.041)
12. Jianming Jiang, Long Yu, Xinghua Huang, Xiaosong Chen, Jie Gao, Yazhou Zhang, Lisha Tang and Shouyuan Zhao. Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B,and their expression changes in HCC tissues from 68 Chinese patients. Gene 2001 27;281(1-2):103-13. (IF=3.041)
13. Dai F, Yu L, He H, Chen Y, Yu J, Yang Y, Xu Y, Ling W, Zhao SHuman serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK-3beta) at serine-9 through direct interaction.Biochem Biophys Res Commun. 2002 May 17;293(4):1191-6. (IF=2.946)
14. jianqiang yu ,long yu ,zheng chen, lihua zheng, xiaoson chen, xiang wang, damin ren ,shouyuan zhao protein inhibitor of neuronal nitric oxide synthase interacts with protein kinase a inhibitors molecular brain research.2002.99：145-149 (IF=2.538)
15. Chiyuan Chen, Long Yu, Pingzhao Zhang, Jianming Jiang, Yazhou Zhang, Yong Zhao,Jie Gao, Qianhong Wu and Shouyuan Zhao.Human neuronal calcium senor-1(NCS-1) is abundant in the brain and shows the highest expression level in cerebral cortex Neuroscience Letter 2002，319：67－70 (IF=2.021)
16. J. Yang, L. Yu, A. Bi, S. Zhao. Assignment of the human reticulon 4 gene (RTN4) to chromosome 2p14-2p13 by radiation hybrid mapping. Cytogenet Cell Genet. 2000.88:101-102. (IF=1.271)
17. R. Gong, L. Yu, H. Zhang, Q. Tu, Y. Zhao, J. Yang, Y. Xu, S. Zhao. Assignment of human GADD45G to chromosome 9q22.1-q22.3 by radiation hybrid mapping. Cytogenet Cell Genet. 2000.88:95-96 . (IF=1.271)
18. Wangchang Cui, Long Yu, Hua He, Yaya Chu, Jie Gao, Bo Wan, Lisha Tang and Shouyuan Zhao. Cloning of Human Myeloid-associated Differentiation Marker (MYADM) Gene Whose Expression was Up-Regulation in NB4 Cells Induced by All-Trans Retinoic Acid. Molecular Biology Reports 2002，28：123-138 (IF=1.281)
19. Zhiyi Li, Long Yu, Yazhou Zhang, Jie Gao, Pingzhao Zhang, Bo Wan, Chiyuan Chen and Shouyuan Zhao. Identification of human ,mouse and rat PPP1RL,Protein phosphatase-1 inhibitor like,and mapping human PPP1RL to chromosome 19q13.13-q13.2 Molecular Biology Reports 2001，28：91-101 (IF=1.281)
20. Jisong Peng, Long Yu, Masahisa Horiuchi, Pingzhao Zhang, Xinghua, Huang, Yazhou Zhang, Dan Li, Md Abdul Jalil & Shouyuan Zhao. Identification of human CDV-1R and mouse Cdv-1R, two novel proteins with putative signal peptides, especially highly expressed in testis and increased with the male sex maturation. Molecular Biology Reports.2002.29：353-362 (IF=1.281)
21. Qiping Zheng, Long Yu, Honglai Zhang, Qiang Fu,Ninghui Mao , Peirong Hu,Zhengcheng Geng&Shouyuan Zhao. Structure Charaterization of Human RalGDS Gene,and The Identification of Its Variant.Molcular Biology Reports 2000;27(4):209-16 (IF=1.281)
22. Qing Liu, Long Yu,Jie Gao,Qiang Fu,Jianxuan Zhang,Pingzhao Zhang,Jian Chenand Shouyuan Zhao .Cloning , tissue expression pattern and genomic organization of latexin ,a human homologue of rat carboxypeptidase A inhibitor Molcular Biology Reports 2001，27：217-214 (IF=1.281)
23. Qing Liu, Long Yu, Qiang Fu, Tieming Lang, Jianxuan Zhang, Daqin Mao, Shouyuan Zhao. Molecular cloning and tissues expression analysis of the βsubunit of elongation factor 1 in mouse. Biochemical Genetics. 2000,38(5/6). (IF=0.625)
24. Chunling Jiang, Long Yu, Yong Zhao, Min Zhang, Qin Liu, Ninghui Mao, Zhengcheng Geng, Shouyuan Zhao. Cloning and characterization of CIS1b(Cytokine inducible SH2-containing protein 1b), an alternative splicing form of C1S1 gene. DNA sequence.2000.11(1-2):149-154. (IF=0.594)
25. Fan Y, Yu L, Tu Q, Gong R, Jiang Y, Zhang Q, Dai F, Chen C, Zhao S.Molecular cloning, genomic organization, and mapping of beta 4GalT-VIb, a brain abundant member of beta 4-galactosyltransferase gene family, to human chromosome 18q12.1. DNA Seq. 2002 Feb;13(1):1-8. (IF=0.594)
26. ZHAO Yong, YU Long, GAO Jie, FU Qiang, HUA Yimin, ZHANG Honglai, ZHAO Shouyuan. cDNA Cloning, Chromosome mapping and Expression Characterization of Human Geranylgerany1 Pyrophosphate Synthase. Science in China. 2000.43(6):614-622. (IF=0.396)
27. Zhang Honglai, Yu Long, Wang Xiaoke, Chen Zheng, Tu Qiang, Cheng Jinqiu, Ding Jianbo, Gao Jie, Zhao Shouyuan. Cloning, characterization and mapping of human SPIN to human chromosome 9q22.1-22.3. Chinese Science Bulletin. 2000,45(10):909-914. (IF=0.511)
28. LAN Fei, YU Long, HU Peirong, MAO Daqin, LI Xiaoquan, ZHANG Min,HUANG Hai, ZHAO Shouyuan. The cDNA cloning, tissue expression pattern, and chromosome localization of human protein kinase Bγ gene. Chinese Science Bulletin. 2000.45(20):1832-1839. (IF=0.511)
29. Bi Anding, Yu Long, Yang Jun, Zhang Min, Zhou Yi, Zhao Shouyuan. Cloning and expression analysis of human reticulon 4C cDNA. Chinese Science Bulletin. 2000.45(20):1862-1868 (IF=0.511)
30. JIANG Juxiang, YU Long, ZHANG Honglai, CHEN Zheng, TU Qiang, JIANG Ying, ZHAO Shouyuan. Cloning, expression and mapping of the full-length cDNA of human CCTβ subunit. Chinese Science Bulletin. 2000.45(22):2034-2041. (IF=0.511)
31. .HU Peirong, YU Long, ZHANG Min, ZHENG Lihua, LAN Fei, Fu Qiang, ZHAO Shouyuan. Cloning, tissue expression pattern characterization and chromosome localizaion of human peptide methionine sulfoxide reductase cDNA. Chinese Science Bulletin.2000.45(24) (IF=0.511)
32. Fan Yunxin, Yu Long, Jiang Ying, Xu Yuefang, Yang Renmin, Han Yongzhu, Cui Yingyu, Ren Mingshan, Zhao Shouyuan. Identification of a mutation hotspot in exon 8 of Wilson desease gene by cycle sequencing. Chinese Medical Journal. 2000, 113(2),172-.174. (IF=0.108)
33. Wang J, Zhao CY, Si YM, Liu ZL, Chen B, Yu L ACT and UCH-L1 polymorphisms in Parkinson"s disease and age of onset. Mov Disord. 2002 Jul;17(4):767-71 (IF=2.561)
以联系作者发表的核心论文
1. Guo JH, Zhu PY, Huang YF, Yu L. Autosomal aberrations associated with testicular dysgenesis or spermatogenic arrest in Chinese patients.
Asian J Androl. 2002 Mar;4(1):3-7.
2. Liu Q, Yu L, Han XF, Fu Q, Zhang JX, Tang H, Zhao SY. [Cloning and tissue expression pattern analysis of the human citrate synthase cDNA]
Shi Yan Sheng Wu Xue Bao. 2000 Sep;33(3):207-14. Chinese.
3. Jiang CL, Yu L, Zhang HL, Zhang M, Fu Q, Zhao Y, Geng ZC, Zhao SY. [Cloning and tissue expressive pattern analysis of the human ribosomal S6 kinase-RPS6KA5 cDNA]
Shi Yan Sheng Wu Xue Bao. 2000 Jun;33(2):119-27. Chinese.

以合作者发表的SCI论文
1.Wu ZY, Wang N, Lin MT, Fang L, Murong SX, Yu L .Mutation analysis and the correlation between genotype and phenotype of Arg778Leu mutation in chinese patients with Wilson disease.. Arch Neurol 2001 58(6):971-6 (IF=4.053)

2.YANG Peng-Yuan, RUI Yao-Cheng, LI Kai, HUANG Xing-Hua, JIANG Jian-Ming, YU Long Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin Acta pharmacol sin 2002 Apr:23(4):327-330 (IF=0.631)