王启要
海洋生物学专业：硕士生导师
生物化学与分子生物学：硕士生导师
生物工程领域：生物工程硕士生导

1978年5月生，男，博士，华东理工大学生物工程学院，生物反应器工程国家重点实验室副教授，硕士生导师。

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主要研究方向：
1.采用分子系统学方法研究动物病原性细菌致病机制
主要针对我国海水养殖病原性细菌的典型流行菌株，采用生化分离纯化、转座子随机突变体文库、突变体确定文库以及组学（基因组学、蛋白质组学和RNA组学）等策略鉴定其毒力因子，然后采用信号传导研究方法研究其受胞内外以及宿主各种信号/胁迫下的各种调控机制，描绘病原菌在致病过程以及环境适应过程中不同的毒力调控网络，从而为防治药物和疫苗的设计和组装提供靶标和相关分子器件。目前主要研究兴趣是迟钝爱德华氏菌和溶藻弧菌中三型分泌系统（TTSS）和六型分泌系统（T6SS）及其调控机制研究。目前这些研究工作与国外大学（如美国宾夕法尼亚大学、哈佛医学院、威斯康辛大学、瑞典UmeåUniversity、英国诺丁汉大学等）相关课题组联系较为紧密。
2.采用合成生物学方法用于疫苗开发
主要针对海洋养殖动物病原迟钝爱德华氏菌进行疫苗的开发工作，并且进行实际可行的多联疫苗的应用研究。这些研究与国内水产养殖相关院校、研究所和企业紧密合作。
目前承担和参加的科研项目：
1.国家863计划重点项目“迟钝爱德华氏菌毒力相关功能基因研究及疫苗开发”，2008AA092501，2009.1-2013.12.
2.国家自然科学基金项目“VI型分泌系统（T6SS）在溶藻弧菌中调控碱性丝氨酸蛋白酶合成/分泌的分子机制”，30901113，2010-2012.
3.国家863计划重点项目“海洋微生物大规模发酵制备候选药物关键技术”，2011AA090702,2011.07-2014.06
4.农业部国家鲆鲽类产业技术体系“疾病防控岗位”，CARS-50,2011-2015
本科和研究生教学：
1.生物科学专业大三第2学期课程，生物信息学，32学时，2学分，专业必修课；
2.生物工程专业博士研究生课程：微生物细胞信号转导，32学时，2学分，专业选修课；
3.2008级生物技术081班班导师；2012级生物技术121班班导师。
学术兼职：
1.澳大利亚微生物学会（ASM）会员，2011-现在
2.JApplMicrobiol,FishShellfishImmun,PLOSOne,AfricanJournalofMicrobiologyResearch(AJMR),ChineseJournalofOceanologyandLimnology,AntonievanLeeuwenhoekJournalofMicrobiology等杂志审稿人
发表论文：
2009年9月1日以后的成果(通讯作者或第一作者)
1.JingfanXiao,QiyaoWang*,QinLiu,LiliXu,XinWang,HaizhenWu,andYuanxingZhang*CharacterizationofEdwardsiellatardarpoS:effectonserumresistance,chondroitinaseactivity,biofilmformation,andautoinducersynthetasesexpression,AppliedMicrobiologyandBiotechnology,2009,83:151-160.
2.QiyaoWang,MinjunYang,JingfanXiao,HaizhenWu,XinWang,YuanzhiLv,LiliXu,HuajunZheng,ShengyueWang,GuopingZhao,QinLiu,YuanxingZhang.GenomesequenceoftheversatilefishpathogenEdwardsiellatardaprovidesinsightsintoitsadaptationtobroadhostrangesandintracellularniches.PLoSONE,2009,Oct.17,4(10):e7646.
3.XinWang,QiyaoWang*,JingfanXiao,QinLiu,HaizhenWu,LiliXu,YuanxingZhang*.EdwardsiellatardaT6SScomponentevpPisregulatedbyesrBandiron,andplaysessentialrolesintheinvasionoffish.Fish&ShellfishImmunology,2009,Sept.27(3):469-477.
4.XinWang,QiyaoWang*,MinjunYang,JingfanXiao,QinLiu,HaizhenWu,andYuanxingZhang*.QseBCcontrolsflagellarmotility,fimbrialhemagglutinationandintracellularvirulenceinfishpathogenEdwardsiellatarda.Fish&ShellfishImmunology.2011,30:944-953
5.HuanLiu,?QiyaoWang*,QinLiu,XiaodanCao,CunbinShi,?YuanxingZhang*.RolesofHfqinthestressadaptationandvirulenceinfishpathogenVibrioalginolyticusanditspotentialapplicationasatargetforliveattenuatedvaccine,AppliedMicrobiologyandBiotechnology,2011,91:353–364
6.JingfanXiao,TaoChen,QiyaoWang*,QinLiu,XinWang,YuanzhiLv,HaizhenWu,andYuanxingZhang*.Searchforliveattenuatedvaccinecandidateagainstedwardsiellosisbymutatingvirulence-relatedgenesoffishpathogenEdwardsiellatarda.LettersinAppliedMicrobiology.2011,Jun.53(4):430-437
7.YaminWang,QiyaoWang*,JingfanXiao,QinLiu,andYuanxingZhang.GeneticRelationshipsofEdwardsiellaStrainsIsolatedinChinaAquacultureRevealedbyrep-PCRGenomicFingerprintingandInvestigationofEdwardsiellaVirulenceGenes.JournalofAppliedMicrobiology,2011,111(6):1337-1348
8.HuanLiu,DanGu,XiaodanCao,QinLiu,QiyaoWang*,YuanxingZhang*.CharacterizationofanewquorumsensingregulatorluxTanditsrolesintheextracellularproteaseproduction,motilityandvirulenceinfishpathogenVibrioalginolyticus.ArchivesofMicrobiology.2012,194:439-452
9.LiliSheng,QiyaoWang*,QinLiu,YuanxingZhangConnectionbetweenTypeVISecretionSystem,QuorumSensing,andAlternativeSigmaFactorinFishPathogenVibrioalginolyticus.ArchivesofMicrobiology,2012,194(5):379-390
10.YuanzhiLv,,JingfanXiao,QinLiu,HaizhenWu,YuanxingZhang,QiyaoWang*.Systematicmutationanalysisoftwo-componentsignaltransductionsystemsrevealsEsrA-EsrBandPhoP-PhoQasthemajorvirulenceregulatorsinEdwardsiellatarda.VeterinaryMicrobiology.2012,157:190-199
11.JingfanXiao,TaoChen,QiyaoWang*,YuanxingZhang.ComparativeanalysisoftherolesofcatalasesKatBandKatGinthephysiologicalfitnessandpathogenesisoffishpathogenEdwardsiellatarda.LettersinAppliedMicrobiology.2012,54,425-432
12.MinjunYang,JingfanXiao,HaizhenWu,YuanzhiLv,HuajunZheng,QinLiu,YuanxingZhang,QiyaoWang*.Edwardsiellacomparativephylogenomicsrevealthenewintra/inter-speciestaxonomicrelationships,virulenceevolutionandnicheadaptationmechanisms.PLOSONE.2012,7(5):e36987.
13.JingfanXiao,XinWang,TaoChen,YuanxingZhang,QiyaoWang*.IdentificationofqseEGFgeneticlocusanditsrolesinregulatinghemolyticactivityandinvasioninfishpathogenEdwardsiellatarda.LettersinAppliedMicrobiology,2012,doi:10.1111/j.1472-765X.2012.03260.x
14.HuanLiu,DanGu,LiliSheng,QiyaoWang*,YuanxingZhang.InvestigationoftherolesofT6SSgenesinmotility,biofilmformation,andextracellularproteaseAspproductioninVibrioalginolyticuswithmodifiedGateway-compatibleplasmidsLettersinAppliedMicrobiology,2012,55:73-81
15.YuanzhiLv,JunyuanZheng,MinjunYang,QiyaoWang*,YuanxingZhang.AnEdwardsiellatardamutantlackingUDP-glucosedehydrogenaseshowspleiotropicphenotypes,attenuatedvirulence,andpotentialasavaccinecandidate.VetMicrobiol,2012,doi:10.1016/j.vetmic.2012.06.006.
16.LiliSheng,YuanzhiLv,QinLiu,QiyaoWang*,YuanxingZhang.ConnectingtypeVIsecretion,quorumsensing,andc-di-GMPproductioninfishpathogenVibrioalginolyticusthroughphosphatasePppA,VetMicrobiol,2012,doi:10.1016/j.vetmic.2012.09.009
17.YaminWang,WeizhengYang,QiyaoWang*,YuanxingZhang.ProteomicscharacterizationofEdwardsiellatardatwin-argininetranslocationsystem:itsessentialrolesinresponsetohighosmoticstressandpotentialuseasanovelbiologicalcontainmentforliveattenuatedvaccineinmarinefishvaccinology.ApplMicrobiolBiotechnol,2012,revised
18.MinjunYang,QiyaoWang*,HaizhenWu,JingfanXiao,QinLiu,andYuanxingZhangPhylogeneticinvestigationofEdwardsiellatardawithmultilocussequencetyping(MLST)andpulsedfieldgelelectrophoresistyping(PFGE)methods,JApplMicobiol,2012,submitted
参与的主要论文：
1.DahaiYang,QinLiu*,MinjunYang,HaizhenWu,QiyaoWang,JingfanXiao,YuanxingZhang*.RNA-seqlivertranscriptomeanalysisrevealsanactivatedMHC-IpathwayandaninhibitedMHC-IIpathwayattheearlystageofvaccineimmunizationinzebrafish.BMCGenomics,2012,13:319
2.MengZhang,HaizhenWu*,XiaoyongLi,MinjunYang,TaoChen,QiyaoWang,QinLiu,YuanxingZhang.EdwardsiellatardaflagellarproteinFlgD:Aprotectiveimmunogenagainstedwardsiellosis.Vaccine,2012,30:3849-3856
3.ZhihuiZhang,HaizhenWu*,JingfanXiao,QiyaoWang,QinLiu,YuanxingZhang.Immuneresponsesofzebrafish(Daniorerio)inducedbybath-vaccinationwithaliveattenuatedVibrioanguillarumvaccinecandidate.Fish&ShellfishImmunology,2012,33:36-41
4.XinWang,QiyaoWang,JingfanXiao,QinLiu*,HaizhenWu,YuanxingZhang*.HemolysinEthAinEdwardsiellatardaisessentialforfishinvasioninvivoandinvitroandregulatedbytwo-componentsystemEsrA-EsrBandnucleoidproteinHhaEt.Fish&ShellfishImmunology.2010,29:1082-1091
5.LingyuGuan,WeiMu,JonathanChampeimont,QiyaoWang,HaizhenWu,JingfanXiao,WernerLubitz,YuanxingZhang,QinLiu*.Iron-RegulatedLysisofRecombinantEscherichiacoliinHostReleasesProtectiveAntigenandConfersBiologicalContainment.Infectionandimmunity,2011,79:2608-2618
6.YanZhao,QinLiu*,XiuhuaWang,LingyunZhou,QiyaoWang,YuanxingZhang*.SurfaceDisplayofAeromonashydrophilaGAPDHinAttenuatedVibrioanguillarumtoDevelopaNovelMultivalentVectorVaccine.MarBiotechnol2011,13:963-970.
7.ZhaoYang,QinLiu,*QiyaoWang,YuanxingZhang*.NovelBacterialSurfaceDisplaySystemsBasedonOuterMembraneAnchoringElementsfromtheMarineBacteriumVibrioanguillarum.ApplEnvironMicrobiol.2008,74,4359-4365
8.XiaodanCao,QiyaoWang,QinLiu*,HaopengRui,HuanLiu,YuanxingZhang*.IdentificationofaluxO-regulatedextracellularproteinPepanditsrolesinmotilityinVibrioalginolyticus.MicrobialPathogenesis,2011,50:123-131
出版专著：
王启要,刘琴,张元兴.群体感应——细菌之间的语言.生物科学与工程(叶勤主编),北京,科学出版社,2008,43-67.
发明专利申请：
1刘琴,张元兴,杨朝,王启要.细菌表面展示新系统、方法及应用.公开号:CN101182516,2008.05.21
2刘琴,张元兴,杨朝,王启要.新型细菌表面展示系统、方法及应用.公开号:CN101195823,2008.06.11
3张元兴,王鑫,王启要,刘琴.一种迟钝爱德华氏菌野生毒株的无标记基因缺失减毒突变株及其应用,200910052707.6
4王启要,张元兴,刘欢,刘琴.一种溶藻弧菌减毒株的无标记基因缺失减毒突变株、相关制剂其应用,2010092100524200，申请日：2010.09.21；公开日：2011年1月19日
5王启要,张元兴，刘琴，王鑫，迟钝爱德华氏菌野生毒株的无标记基因缺失减毒株、相关制剂及其应用,201010541646.2
6刘琴，张元兴，周凌云，王启要.一种多效价活疫苗、其制备方法及应用.中国发明专利.申请号：200910054113.9
7王启要,刘琴,吴海珍,肖婧凡,张元兴.迟钝爱德华氏菌天然弱毒株及其应用.中国发明专利.申请号：201210240313.5
8王启要,刘琴,张元兴,王亚敏,吴海珍,肖婧凡.迟钝爱德华氏菌突变株及其应用,PCT国际专利:PCT/CN2012/076961
会议论文或报告：
1王启要.迟钝爱德华氏菌的基因组及比较基因组.大会报告.第三届全国微生物基因组学学术研讨会.2010.11.26．厦门
2QiyaoWang,YuanxingZhang.ComparativegenomicsoffishpathogenEdwardsiellatarda(Posterandabstract).AustraliaSocietyofMicrobiology2012YearMeeting,Brisbane,2012,7.1.
3QiyaoWang,YuanxingZhang.HemolysinEthAinEdwardsiellatardaisessentialforfishinvasioninvivoandinvitroandregulatedbytwo-componentsystemEsrA-EsrBandnucleoidproteinHhaEt.(Posterandabstract).Host-PathogenInteractionConference,Amsterdan,2010,9,4

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