金礼吉
院系：生命科学与技术学院
办公电话：**
电子信箱：jinliji2005@yahoo.com.cn
更新时间：2013-4-23
其他专业：生物化工



个人简介
男，博士。大连理工大学化工与环境生命学部生命科学与技术学院，副教授；“动物性食品安全保障技术教育部工程研究中心”副主任。主要研究领域包括抗生素替代品的研制与开发（采用卵黄抗体和噬菌体技术）、天然药效活性物质的分离鉴定及活性研究；染疫动物尸体及养殖废弃物的无害化处理；海水养殖水体的净化；荧光染料在蛋白、多肽分析及细胞信号通路和小分子物质检测中的应用等。先后承担和参与国家自然科学基金委杰出青年科学基金、重大国际合作项目、面上项目，科技部863项目、辽宁省和大连市基金以及国家发改委高新技术产业化示范项目等共9项。发表研究论文150多篇，申请国家发明专利18项，其中授权9项。“特异性卵黄抗体替代抗生素防控动物疾病并保障食品安全”于2012年12月通过国家教育部科技成果鉴定，专家一致认为该成果达到了国际同类研究的领先水平。
主要学历及工作经历:
2002.07 ~ 至今　大连理工大学化工与环境生命学部生命科学与技术学院，副教授。
1998.03 ~ 2002.06　大连理工大学　生物化工专业　博士
1995.11 ~ 1998.02 宝生物工程（大连）有限公司工程师
1994.10 ~ 1995.10　日本宝酒造中央研究所　　　　 研修
1991.09 ~ 1994.06　辽宁师范大学　植物生理专业 　硕士
1987.08 ~ 1991.08 抚顺市朝鲜族第一中学 教师
1983.09 ~ 1987.07 辽宁师范大学生物系 本科


研究领域（研究课题）
生物化学与分子生物学；化学生物学；细胞生物学
在研项目：
1.应用载体固定微生物高效净化集约化海水养殖水体的研究，辽宁省自然科学基金（**），2013.1~2014.12
2.堆肥法治理养殖业废弃物专用微生物制剂功能菌株的分离和筛选，“十二五”农村领域国家科技计划课题（2013AA102805-03），2013.1~2017.12
承担或参与完成项目：
1.染疫动物无害化处理过程中病原微生物消失规律的研究，国际重大合作项目（**），2007.1~2010.12
2.微切变-助剂互作技术生产植物型刺参免疫调节剂和水体修复剂，国家863计划项目（2006AA10Z412），2007.1~2010.12
3.抗非典型肺炎（SARS）冠状病毒特异性卵黄免疫球蛋白的研究开发，大连市科技计划项目(2003B3NS024) ，2003.1~2003.12
4.食品及生活垃圾中有害生物风险分析及无害化处理技术研究，大连市科技计划项目（2009E11SF133），2010.1~2012.12
5.微切变-助剂互作技术制备鹿茸、鹿角冒、鹿胎粉及其功效与成分分析，大连鹿成生物工程有限公司,2009.1~2010.12



硕博研究方向
指导硕、博士生研究方向:
1. 基因的克隆、表达与调控
2. 抗生素取代品的研制与开发（运用抗体技术、噬菌体技术、天然药效活性物质、微生态制剂等防控畜禽类和水产动物疾病）
3. 分子荧光探针（荧光染料在蛋白、多肽分析及细胞内信号通路和小分子检测中的应用）


出版著作和论文
近五年发表论文：
1.Jin L.J., Wu F.F., Li X.Y., Li H.Q., Du C., Jiang Q., You J.S., Li S.Y., Xu Y.P.*. (2013) Anti-depressant effects of aqueous extract from Acanthopanax senticosus in mice. Phytotherapy Research. doi: 10.1002/ptr.4938. (IF:2.086)
2.Cui N.Z., Xu Y.P., Cao Z.H., Xu F.X., Zhang P., Jin L.J. *. (2013). Effects of heat stress on the level of heat shock protein 70 on the surface of hepatocellular carcinoma Hep G2 cells: implications for the treatment of tumors. Tumor Biology. 34(2):743-748. (IF:2.143)
3.Jin L.J., Li H.Q., Wu F.F., Li X.Y., Yu H.B., Cui N.Z., You J.S., Cao Z.H., Sun X.H., Zhang J.C, Wang X.L., Song C.N., Li S.Y., Xu Y.P.*(2012). Application of mechanochemical pretreatment prior to aqueous extraction of Eleutheroside B from Eleutherococcus senticosus. Industrial Engineering Chemistry Research. 51:10695-10701. (IF:2.071)
4.Jin L.J., Li X.Y., Zou D.L., Li S.Y., Song W.Q., Xu Y.P.* (2012). Protection of crucian carp (Carassius auratus Gibelio) against septicaemia caused by Aeromonas hydrophila using specific egg yolk immunoglobulins. Aquaculture Research, 1-9. (IF:1.186)
5.Yu H.B., Xiao Y.*, Jin L.J.*(2012).A Lysosome-Targetable and Two-Photon Fluorescent Probe for 2 Monitoring Endogenous and Exogenous Nitric Oxide in Living Cells. Journal of the American Chemical Society. 134 (42):17486-17489. (IF:9.907)
6.Li X.Y., Liu H., Xu Y.P., Xu F.X., Wang L.H., You J.S., Li S.Y., Jin L.J.* (2012). Chicken egg yolk antibody (IgY) controls Solobacterium moorei under in vitro and in vivo conditions. Applied Biochemistry and Biotechnology,168(6):1448-1458. (IF:1.943)
7.Yongping Xu, Xiaoyu Li, Liji Jin*, Yuhong Zhen, Yanan Lu, Shuying Li,Jiansong You, Linhui Wang. (2011). Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: A review. Biotechnology Advances. 29(6): 860-868. (IF:9.646)
8.You J.S., Xu Y.P., He M., McAllister T.A., Thacker P.A., Li X.Y., Wang T.T., Jin L.J.* (2011). Protection of mice against enterotoxigenic E. coli by immunization with a polyvalent enterotoxin comprising a combination of LTB, STa, and STb. Applied Microbiology and Biotechnology, 89(6): 1885-1893. (IF:3.280)
9.HongYu Xue, DongYan Niu, GuiZhen Gao, QiuYe Lin, LiJi Jin*, YongPing Xu. (2011). Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H2O2-induced PC12 cells. Molecular Biology Reports. 38(5): 3561-3567. (IF:1.875)
10.Tingting Wang , Yongping Xu , Wenjia Liu , Yongxin Sun , Liji Jin*. (2011). Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris. Protein Expression and Puri?cation. 77(1): 20-25. (IF:1.644)
11.Zhang P., Xu Y.P., Sun J.X., Li X.Y., Wang L.H., Jin L.J.* (2009). Protection of pyrroloquinoline quinone against methylmercury-induced neurotoxicity via reducing oxidative stress. Free radical research. 43(3): 224-233. (IF: 2.805)
12.Zhang P., Xu Y.P., Li L.K., Jiang Q., Wang M.J., Jin L.J.* (2009). In vitro protective effects of pyrroloquinoline quinone on methylmercury–induced neurotoxicity. Environmental Toxicology and Pharmacology. 27(1): 103-110. (IF:1.378)
13.Zhang Z.C., Zhang J., Jin L.J*.,Song T., Wu G.Y., Gao J.(2008).Tanshinone IIA interacts with DNA by minor groove-binding.Biological and Pharmaceutical Bulletin.31(12): 2342-2345. (IF: 1.81)
14.Jin L.J., Zhang Z.C.*, Wang Y.Y., Wei M.J., Xu Q. (2007). A novel small molecule inhibitor targeted at Bcl-2. Science in China Series C: Life Sciences, 50(5): 624-629. (IF:0.661)
15.Jin L.J., Dai F., An L.J. (2002). Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal 11:79-85.( IF:2.645)
16.Jin L.J., Dai F., Gao X.R., Wang R.H., An L.J. (2001). Telomerase activity, TRAP, DNA sequence analysis Study on the Detection of Telomerase Activity by Combining DNA Sequence Analysis with TRAP. High Technology Letters. 7(3): 8-10.
17.Lin-Hui Wang, Xiao-Yu Li,Li-Ji Jin, Jian-Song You, Ye Zhou, Shu-Ying Li, Yong-Ping Xu*. (2011). Characterization of chicken egg yolk immunoglobulins (IgYs) speci?c for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus. Veterinary Microbiology. 149(3-4): 415-421.(IF:3.256)
18.Y.-H. Zhen, R. Fang, C. Ding, L.-J. Jin, X.-Y. Li, Y.-P. Diao, X.-H. Shu, X.-C. Ma, Y.-P. Xu. (2011). Ef?cacy of speci?c IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model. Journal of Applied Microbiology. 111(6): 1524-1532. (IF:2.365)
19.Zhen Y.H., Jin L.J., Li X.Y., Guo J., Li Z., Zhang B.J., Fang R., Xu Y.P. * (2009). Efficacy of specific egg yolk immunoglobulin (IgY) to bovine mastitis caused by Staphylococcus aureus. Veterinary Microbiology. 133(4):317-322.(IF:2.370)
20.Li X.Y., Jin L.J., Uzonna, J.E., Li, Sh.Y., Liu J.J., Li H.Q., Lu Y.N., Zhen Y.H., Xu Y.P.* (2009). Chitosan–alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY): In vivo evaluation in a pig model of enteric colibacillosis. Veterinary Immunology and Immunopathology. 129(1-2): 132-136. (IF:1.907)
21.Li X.Y., Jin L.J., Lu Y.N., Zhen Y.H., Li S.Y., Wang L.H, Xu Y.P.* (2009). Chitosan–alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY): Effects of chitosan concentration. Applied Biochemistry and Biotechnology. 159(3):778-787 (IF: 1.04)
22.Wang T.T., Sun Y.X., Jin L.J., Xu Y.P.*, Wang L., Ren T.J., Wang K.L. (2009) Enhancement of non-specific immune response in sea cucumber (Apostichopus japonicus) by Astragalus membranaceus and its polysaccharides. Fish and Shellfish Immunology. 27(6):757-762 (IF:3.161)
23.Lu Y.N., Liu J.J., Jin L.J., Li X.Y., Zhen Y.H., Xue H.Y., Lin Q.Y., Xu Y.P.* (2009) Passive immunization of crayfish (Procambius clarkiaii) with chicken egg yolk immunoglobulin (IgY) against white spot syndrome virus (WSSV). Applied Biochemistry and Biotechnology. 159(3):750-758
24.Xue H.Y., Jin L., Jin L.J., Li X.Y., Zhang P., Ma Y.Sh., Lu Y.N., Xia Y.Q., Xu Y.P. * (2009). Aucubin prevents loss of hippocampal neurons and regulates antioxidative activity in diabetic encephalopathy rats. Phytotherapy Research. 23(7): 980-986. (IF:1.772)
25.Xue J.P., Xu Y.P.*, Jin L.J., Liu G., Sun Y.X., Li S.Y., Zhang J.C. (2008). Effects of traditional Chinese medicine on immune responses in abalone, Haliotis discus hannai Ino. Fish and Shellfish Immunology. 24(6):752-758. (IF: 3.160)
26.Zhen Y.H., Jin L.J., Guo J., Li X.Y., Li Z., Fang R., Xu Y.P.* (2008). Charcterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. Journal of Applied Microbiology. 105(5): 1529-1535. (IF:2.028)
27.Sun Y.X., Jin L.J., Wang T.T., Xue J.P., Liu G., Li X.Y., You J.S., Li S.Y., Xu Y.P.* (2008). Polysaccharides from Astragalus membranaceus promote phagocytosis and superoxide anion (O2?) production by coelomocytes from sea cucumber Apostichopus japonicus (Selenka) in vitro. Comparative Biochemistry and Physiology C. 147(3): 293-298. (IF:2.345)
28.Lin Q.Y., Jin L.J., Cao Z.H., Xu Y.P. * (2008). Protective effect of Acanthopanax senticosus against endotoxic shock in mice. Journal of Ethnopharmacology. 118(3): 495-502.(IF:2.049)
29.Lin Q.Y., Jin L.J., Cao Z.H., Xu Y.P.* (2008). Inhibition of inducible nitric oxide synthase by Acanthopanax senticosus extract in RAW264.7 macrophages. Journal of Ethnopharmacology. 118(2):231-236. (IF:2.049)
30.Zhen Y.H., Jin L.J., Guo J., Li X.Y., Lu Y.N., Chen J., Xu Y.P.* (2008). Charcterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Escherichia coli. Veterinary Microbiology. 130(1-2):126-133. (IF:2.010)
31.Lin Q.Y., Jin L.J., Cao Z.H., Lu Y.N., Xue H.Y., Xu. Y.P.* (2008). Acanthopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo. Phytotherapy Research. 22(6): 740-745. (IF:1.430)
32.Xue H.Y., Jin L.J., Jin L., Zhang P., Li D. Q., Xia Y.Q., Lu Y.N., Xu Y.P. * (2008). Neuroprotection of aucubin in primary diabetic encephalopathy. Science in China Series C: Life Sciences. 51(6): 495-502. (IF:0.635)
33.Lu Y.N., Liu J.J., Jin L.J., Li X.Y., Zhen Y.H., Xue H.Y., You J.S., Xu Y.P.* (2008). Passive protection of shrimp against white spot syndrome virus (WSSV) using specific antibody from egg yolk of chickens immunized with inactivated virus or a WSSV-DNA vaccine. Fish and Shellfish Immunology. 25(5):604-610. (IF:3.161)
34.Jin L., Xue H.Y., Jin L.J., Li S.Y., Xu Y.P.* (2008). Antioxidant and pancreas-protective effect of aucubin on rats with Streptozotocin-induced diabetes. European Journal of Pharmacology. 582(1-3): 162-167. (IF:2.376)
35.Li X.Y., Jin L.J., McAllister T.A., Stanford K., Xu J.Y., Lu Y.N., Zhen Y.H., Sun Y.X., Xu Y.P.* (2007).Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY). Journal of Agricultural and Food Chemistry, 55(8):2911-2917. (IF:2.532)
36.Xia Y.Q., Jin L.J., Zhang B., Xue H.Y., Li Q.J., Xu Y.P.* (2007). The potentiation of curcumin on insulin-like growth factor-1 action in MCF-7 human breast carcinoma cells. Journal of Life Sciences, 80(23):2161-2169. (IF:2.257)
37.Liu Y., Jin L.J., Li X.Y., Xu Y.P.* (2007). Application of mechanochemical pretreatment (MCPT) to aqueous extraction of isofraxidin from Eleutherococcus senticosus. Industrial ＆ Engineering Chemistry Research.46 (20): 6584 -6589. (IF:1.749)
38.Lin Q.Y., Jin L.J., Ma Y.S., Shi M., Xu Y.P.* (2007). Acanthopanax senticosus Inhibits Nitric Oxide Production in Murine Macrophages In Vitro and In Vivo. Phytotherapy Research.21(9):879-883. (IF:1.430)
39.Zhang Z.C.*, Jin L.J., Qian X.H., Wei M.J., Wang Y.Y., Wang J., Yang Y.Y., Xu Q., Xu Y.T., Liu F.Y. (2007) Novel Bcl-2 Inhibitors: Discovery and Mechanism Study of Small Organic Apoptosis-Inducing Agents. ChemBioChem, 8: 113-121. (IF: 3.322)
40.Yang G.L.*, An L.J., Li Q., Lin J.R., Liu W.D., Jin L.J., Lin X.R. (2007) Genotyping of Trichophyton rubrum by analysis of ribosomal-DNA intergenic spacer regions. Mycopathologia, 164:19-25. (IF: 1.652)
41.金礼吉*，王利平，邹薇，肖义。新型双萘酰亚胺类化合物与DNA的作用研究。大连理工大学学报，2013,53(2):171-175
42.金礼吉，张志超，王媛媛，魏美娇，徐芹. 一个全新的有机小分子Bcl-2蛋白抑制剂. 中国科学(C辑:生命科学). 2007(4): 416-421.


工作成果（奖励、专利等）
“特异性卵黄抗体替代抗生素防控动物疾病并保障食品安全”于2012年12月通过国家教育部科技成果鉴定，专家一致认为该成果达到了国际同类研究的领先水平。
授权专利：
1.金礼吉，韩丽丽，徐永平，李晓宇，尤建嵩，王林会，邹栋梁，陆燕，续繁星，巨文娜， 王妮。一种用于水果保鲜的特异性卵黄抗体制剂、制备方法及应用。授权公告日：2009.1.7，专利权人：大连理工大学，专利号：ZL **9.0
2.肖义，金礼吉。甲基异硫脲阳离子作为连接基的脱氧核糖核酸双嵌入剂。授权公告日：2008.1.30，专利权人：大连理工大学，专利号：ZL **5.0
3.徐永平，金礼吉，牛冬燕，刘莹，李晓宇，尤建嵩，林秋叶，姜春生. 一种用于植物有效成分提取方法。授权公告日：2009.5.13，专利权人：大连赛姆生物工程技术有限公司，专利号：ZL **2.3
4.徐永平，尤建嵩，金礼吉，李晓宇，马永生，王林会，李化强，吴菲菲。一种大肠杆菌多个肠毒素基因的融合体及其应用。授权公告日: 2013.3.27；专利权人：大连理工大学; 专利号：ZL **4.2
5.徐永平，马永生，金礼吉，李淑英，李晓宇，尤建嵩，曹振辉，谭德猛。一种噬菌体口服微球制剂及其制备方法。授权公告日：2012.8.30，专利权人：大连理工大学，专利号：ZL **5.3
6.徐永平，李建光，金礼吉，李晓宇，谭德猛，王黎，汪将，车鉴。一种环保型海参条索状饲料及其制备方法。授权公告日：2012.9.12，专利权人：大连理工大学，专利号：ZL **0.2
7.徐永平，李淑英，杨连玉，金礼吉，林秋叶，尤建嵩，刘莹，牛冬燕。刺五加饲料添加剂和制备方法。授权公告日：2011.5.11，专利权人：大连赛姆生物工程技术有限公司，专利号：ZL 2005 1 **.3
8.徐永平，孙永欣，刘钢，汪婷婷，金礼吉，姜志强。一种植物型刺参复方免疫促进剂。授权公告日：2010.6.9，专利权人：大连理工大学，专利号：ZL **3.3
9.徐永平，刘钢，孙永欣，金礼吉，薛继鹏，汪婷婷，王开来。一种皱纹盘鲍用复方中草药诱食及免疫增强剂。授权公告日：2010.2.3，专利权人：大连理工大学，专利号：ZL **5.2


在读学生人数
8

毕业学生人数
13