于海宁
院系：生命科学与技术学院
办公电话：
电子信箱：yuhaining@dlut.edu.cn
更新时间：2015-6-8
其他专业：无



个人简介
1997年9月- 2001年7月北京大学 医学部药学专业 获理学学士学位
2001年8月-2005年12月 University of Iowa, USACollege of Pharmacy, 获理学博士
学位
2006年至今大连理工大学生命科学与技术学院



社会兼职
American Chemical Society美国化学学会会员
American Association of Pharmaceutical Scientists美国药物科学家协会会员
Chinese Association of Pharmaceutical Scientists中国药学会会员
The Chinese Society of Biochemistry and Molecular Biology中国生物化学与分子生物会会员


研究领域（研究课题）
研究方向主要通过蛋白质组学、基因组学结合药理学手段从我国特有海洋动物及药源性动物尤其富含活性蛋白或多肽类生物活性分子的动物类中药中识别功能分子，采用现代分子生物学技术探明其结构、功能、作用靶标及作用机制，着重筛选具有靶标心血管系统(强心, 降压, 抑制或促进血小板聚集，舒缓平滑肌等), 免疫调节系统（免疫抑制与增强），以及靶标病原性微生物、肿瘤细胞的新型活性肽；建立天然药物活性蛋白/多肽分子资源库，挖掘具有重要药用前景的活性蛋白多肽，为生物制药现代化和创新药物研制及其临床应用奠定理论基础。由于各种动物来源的活性肽，活力高，专一性强，和人的生理蛋白质、多肽具有进化上的生物关联性和变异性（序列同源－变异导致功能同源－分化），深入比较研究活性蛋白肽多基因家族分子与人内源性分子构效关系，揭示重要的活性蛋白多肽与人体内相关蛋白质相互作用的规律，发现与确立作用的新靶点；探明活性蛋白多肽的基因起源，与生物靶点的协同进化特征及在生物适应中的意义。

硕博研究方向
主要两个方向：药用动物来源活性多肽在宿主先天免疫中的作用（基础研究）+活性多肽（新药研发）
1.海洋生物活性物质的分离、结构与功能研究及其临床应用；从传统动物中药中识别功能分子，揭示发挥药效的物质基础；
2.探明活性蛋白多肽的基因起源，与生物靶点的协同进化特征及在生物适应中的意义
3.建立天然药物活性蛋白/多肽分子资源库，研发创新药物。


出版著作和论文
论文与著作（共53篇，其中SCI 51篇，国际会议1篇，专著1篇；第一与通讯作者共33篇）
1.Wei L, Gao J, Zhang S, Wu S, Xie Z, Ling G, Kuang Y, Yang Y, Haining Yu (通讯), Wang Y. Identification and Characterization of the First Cathelicidin from Sea Snakes with Potent Antimicrobial and Anti-inflammatory Activity, and Special Mechanism. J Biol Chem. 2015 May 26.

2.Haining Yu(通讯)，Yiling Lu, Xue Qiao, Tingting Fu, Shasha Cai, Chen Wang, Xuelian Liu, Yipeng Wang. Novel Cathelicidins from Pigeon Highlights Evolutionary Convergence in Avain Cathelicidins and Functions in Modulation of Innate Immunity. Scientific Reports

3.Guiying Ling, Jiuxiang Gao, Shumin Zhang, Zeping Xie, Lin Wei, Haining Yu (通讯), Yipeng Wang. Cathelicidins from the bullfrog Rana catesbeiana provides novel template for peptide antibiotic design. PLoS One. 2014 Mar 27;9(3):e93216.
4.Guiying LING, Li LI, Jiuxiang GAO, Haining YU, Yipeng WANG*, Jiang ZHOU* Geographically Distinct Expression Profile of Host Defense Peptides in the Skin of the Chinese Odorous Frog, Odorrana margaretae. Asian Herpetological Research 2013, 4(4): 288–297
5.Liu H, Mu L, Tang J, Shen C, Gao C, Rong M, Zhang Z, Liu J, Wu X, Haining Yu (通讯), Lai R. A potential wound healing-promoting peptide from frog skin. Int J Biochem Cell Biol. 2014 Jan 17;49C:32-41.
6.Haining Yu (通讯), Cai S, Gao J, Zhang S, Lu Y, Qiao X, Yang H, Wang Y. Identification and polymorphism discovery of the cathelicidins, Lf-CATHs in ranid amphibian (Limnonectes fragilis). FEBS J. 2013 Dec;280(23):6022- 32.
7.Haining Yu (通讯), Jiuxiang Gao, Yiling Lu, Xue Qiao, Shasha Cai, Songyan Zhang, Yipeng Wang. Molecular cloning, sequence analysis and phylogeny of first Caudata g-type lysozyme in Axolotl (Ambystoma mexicanum). Zoolog Sci. 2013 Nov;30(11):938-43.
8.Zhang S, Gao J, Lu Y, Cai S, Qiao X, Wang Y, Haining Yu (通讯). Molecular cloning, sequence analysis and homology modeling of the first caudata amphibian antifreeze-like protein in axolotl (Ambystoma mexicanum). Zoolog Sci. 2013 Aug;30(8):658-62.
9.Zheng Li, Songyan Zhang, Huijuan Guang, Jiuxiang Gao, Huanhuan Guo, Weiyu He, Yipeng Wang, Haining Yu (通讯). Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok. Fish & Shellfish Immunology. 2013 Jan; 34(1):1-7
10.Yang S, Liu Z, Xiao Y, Li Y, Rong M, Liang S, Zhang Z, Haining Yu, King GF, Lai R. Chemical punch packed in venoms makes centipedes excellent predators. Mol Cell Proteomics. 2012 Sep;11(9):640- 50.
11.Wang H, Yu Z, Hu Y, Haining Yu, Ran R, Xia J, Wang D, Yang S, Yang X, Liu J. Molecular cloning and characterization of antimicrobial peptides from skin of the broad-folded frog, Hylarana latouchii. Biochimie. 2012 Jun;94(6):1317-26. IF 3.897
12.Zhang S, Guo H, Shi F, Wang H, Li L, Jiao X, Wang Y, Haining Yu (通讯). Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis. Peptides. 2012 Feb;33(2):251-7.
13.Wang H, Ran R, Haining Y, Yu Z, Hu Y, Zheng H, Wang D, Yang F, Liu R, Liu J. Identification and characterization of antimicrobial peptides from skin of Amolops ricketti (Anura: Ranidae).Peptides. 2012 Jan;33(1):27-34.
14.Hao X, Yang H, Wei L, Yang S, Zhu W, Ma D, Lai R, Haining Yu (通讯). Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate. Amino Acids. 2012 Aug;43(2):677- 85.
15.He W, Feng F, Huang Y, Guo H, Zhang S, Li Z, Liu J, Wang Y, Haining Yu (通讯). Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial. Biochimie. 2012 Mar;94(3):649-55.
16.Wang Y, Zhang Z, Chen L, Guang H, Li Z, Yang H, Li J, You D, Haining Yu (通讯), Lai R. Cathelicidin-BF, a Snake Cathelicidin-Derived Antimicrobial Peptide, Could Be an Excellent Therapeutic Agent for Acne Vulgaris. PLoS One. 2011;6(7):e22120.
17.Wu J, Liu H, Yang H, Haining Yu, You D, Ma Y, Ye H, Lai R. Proteomic analysis of skin defensive factors of tree frog Hyla simplex. J Proteome Res. 2011 Sep 2;10(9):4230-40. 18.Zhao H, Kong Y, Wang H, Yan T, Feng F, Bian J, Yang Y, Haining Yu (通讯). A defensin-like antimicrobial peptide from the venoms of spider, Ornithoctonus hainana. J Pept Sci. 2011 Jul;17(7):540-4.
19.Chen W, Yang X, Chen L, Yang X, Feng F, He W, Liu J, Haining Yu (通讯). Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami. Biochimie. 2011 Jul;93(7):1110-4.
20.Yang H, Wang Y, Xiao Y, Wang Y, Wu J, Liu C, Ye H, Li F, Haining Yu (通讯), Lai R. A bi-functional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities. PLoS One. 2011 Mar 14;6(3):e17519
21.Feng F, Chen C, Zhu W, He W, Guang H, LiZ, WangD, LiuJ, Chen M, WangY, Haining Yu (通讯). Gene cloning, expression, and characterization of avian cathelicidin orthologue, Cc-CATHs, from Coturnix coturnix. FEBS Journal. 2011 May;278(9):1573-84.
22.Wang Y, Lu Z, Feng F, Zhu W, Guang H, Liu J, He W, Chi L, LiZ, Haining Yu (通讯). Molecular cloning and characterization of novel cathelicidin-derived myeloid antimicrobial peptide from Phasianus colchicus. Dev Comp Immunol. 2011 Mar;35(3):314-22.
23.Zheng R, Yao B, Haining Yu (通讯), Wang H, Bian J, Feng F. Novel family of antimicrobial peptides from the skin of Rana shuchinae. Peptides. 2010 Sep; 31(9):1674-7.
24.Lu Z, Wang Y, Zhai L, Wang H, Che Q, Du S, Wang D, Feng F, Lai R, Haining Yu (通讯). Novel cathelicidin-derived antimicrobial peptides from Equus asinus. FEBS Journal.2010 May; 277(10):2329- 39.
25.Wu J, Wang Y, Liu H, Yang H, Ma D, Li J, Li D, Lai R, Haining Yu (通讯). Two immunoregulatory peptides with antioxidant activity from tick salivary glands. J Biol Chem. 2010 May 28; 285(22):16606-13.
26.Lu Z, Zhai L, Wang H, Che Q, Wang D, Feng F, Zhao Z, Haining Yu (通讯). Novel families of antimicrobial peptides with multiple functions from skin of Xizang plateau frog, Nanorana parkeri. Biochimie. 2010 May;92(5):475-81.
27.Lu X, Che Q, Lv Y, Wang M, Lu Z, Feng F, Liu J, Haining Yu (通讯). A novel defensin-like peptide from salivary glands of the hard tick, Haemaphysalis longicornis. Prot. Sci. 2010 Mar;19(3):392- 7.
28.Mi X, Haining Yu Jia P, Zhang Z, Zhang L, Liu J. Two tachykinin-like peptides from skin secretions of Danio rerio. J. Pep. Sci. 2010 Feb;16(2):81- 4.
29.Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Haining Yu (通讯), Lai R. Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata. Genomics. 2010 Jan;95(1):66-71.
30.Peng K, Kong Y, Zhai L, Wu X, Jia P, Liu J, Haining Yu (通讯). Two novel antimicrobial peptides from centipede venoms. Toxicon. 2010 Feb-Mar;55(2-3):274-9.
31.Wang H, Yan X, Haining Yu, Hu Y, Chen Z, Zhang Z, Liu J. Isolation, characterization and molecular cloning of new antimicrobial peptides belonging to the brevinin-1 and temporin families from the skin of Hylarana latouchii (Anura: Ranidae). Biochimie. 91(4):540-7(2009).
32.You D, Hong J, Rong M, Haining Yu, Liang S, Ma Y, Yang H, Wu J, Lin D, Lai R. The first gene-encoded amphibian neurotoxin. J Biol Chem. 2009 Aug 14;284(33):22079-86
33.Zhao R, Yu X, Haining Yu, Han W, Zhai L, Han J, Liu J. Immunoregulatory peptides from salivary glands of the horsefly, Tabanus pleskei. Comp Biochem Physiol B Biochem Mol Biol. 2009 Sep;154(1):1-5.
34.Yang X, Wang Y, Lu Z, Zhai L, Jiang J, Liu J, Haining Yu (通讯). A novel serine protease inhibitor from the venom of Vespa bicolor Fabricius. Comp Biochem Physiol B Biochem Mol Biol. 2009 May;153(1):116-20.
35.Wang H, Lu Y, Zhang X, Hu Y, Haining Yu, Liu J, Sun J. The novel antimicrobial peptides from skin of Chinese broad-folded frog, Hylarana latouchii (Anura:Ranidae). Peptides. 2009 Feb;30(2):273-82.
36.Chen W, Yang X, Yang X, Zhai L, Lu Z, Liu J, Haining Yu (通讯). Antimicrobial peptides from the venoms of Vespa bicolor Fabricius. Peptides. 2008 Nov; 29(11):1887-92.
37.Yan X, Feng H, Haining Yu, Yang X, Liu J, Lai R. An immunoregulatory peptide from salivary glands of the horsefly, Hybomitra atriperoides. Dev Comp Immunol. 32(10):1242-7 (2008).
38.Han Y, Haining Yu, Yang X, Rees HH, Liu J, Lai R. A serine proteinase inhibitor from frog eggs with bacteriostatic activity. Comp Biochem Physiol B Biochem Mol Biol. 149(1):58-62 (2008).
39.Lu J, Haining Yu, Gao W, Lai R, Liu J, Liang X. A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides. 29(3):369-74 (2008).
40.Hainig Yu, Wang X, Liu J, Lai R. A novel algesic peptide derived from skin secretions of the frog Amolops loloensis. J Pept Sci. 13(12):798-802 (2007).
41.Haining Yu, Yang H, Ma D, Lv Y, Liu T, Zhang K, Lai R, Liu J. Vespid chemotactic peptide precursor from the wasp, Vespa magnifica (Smith).Toxicon. 50(3):377-82(2007).
42.Li J, Zhang C, Xu X, Wang J, Haining Yu, Lai R, Gong W. Trypsin inhibitory loop is an excellent lead structure to design serine protease inhibitors and antimicrobial peptides. FASEB J. 21(10):2466-73 (2007).
43.Xu C, Ma D, Haining Yu, Li Z, Liang J, Lin G, Zhang Y, Lai R. A bactericidal homodimeric phospholipases A2 from Bungarus fasciatus venom. Peptides. 28(5):969-73 (2007).
44.Li J, Xu X, Xu C, Zhou W, Zhang K, Haining Yu, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 6(5):882-94 (2007).
45.Li J, Haining Yu (并一作者), Xu X, Wang X, Liu D, Lai R. Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami. Genomics. 89(3):413-8 (2007).
46.Lu Y, Li J, Haining Yu, Xu X, Liang J, Tian Y, Ma D, Lin G, Huang G, Lai R. Two families of antimicrobial peptides with multiple functions from skin of rufous-spotted torrent frog, Amolops loloensis. Peptides. 27(12):3085-91 (2006).
47.Xu X, Yang H, Haining Yu, Li J, Lai R. The mastoparanogen from wasp. Peptides. 27(12):3053-7 (2006).
48.D. Yu, J. Liang, Haining Yu, H. Wu, C Xu, J Liu, R. Lai. A tick B-cell inhibitory protein from salivary glands of the hard tick, Hyalomma asiaticum asiaticum. Biochem Biophys Res Commun. 343(2):585-90 (2006).
49.J. Li, X. Xu, Haining Yu, H. Yang, Z. Huang, R. Lai. Direct antimicrobial activities of PR-bombesin. Life Science. 78(17):1953-6 (2006).
50.Haining Yu, E. M. Mu?oz, F. Zhang, R. E. Edens, and R. J. Linhardt.Kinetic studies on the interaction of heparin and complement proteins using surface plasmon resonance. Bioch. Biophy. Acta.1726(2): 168-176 (2005).
51.E. M. Mu?oz, Hainig Yu, J. Hallock, R. E. Edens, and R. J. Linhardt.Poly(ethylene glycol)-based biosensor chip to study heparin protein interactions. Analytical Biochmesitry, 343(1): 176-178 (2005).
52.Haining Yu, F. Zhang,R. E. Edens and R. J. Linhardt. Studies of the interaction between proteoglycan and proteins of biological importance, 226th National Meeting of the American Chemical Society, September 7-11, 2003, New York, NY.
53.Haining Yu, R. E. Edens and R. J. Linhardt.Regulation activity of heparin in complement system, in Chemistry and Biology of Heparin and Heparan Sulfate”, Elsevier Ltd., Oxford, H. G. Garg, R. J. Linhardt, and C. A. Hales (Eds.), Chapter 11, 313-343, 2005.

核心期刊
1.鲁一灵，高久香，乔雪，王义鹏，于海宁*。一种新型改良硫氧还蛋白融合表达体系的建立及cathelicidin家族Lf-CATH2的表达。生物工程学报。2015 .3.25 ，.31(3):415-422
2.广慧娟, 厉政, 王义鹏, 赖仞, 于海宁*. Cathelicidin家族抗菌肽研究进展。Zoological Research。2012，Oct. 33(5): 1?4



工作成果（奖励、专利等）
作为项目负责人承担有国家自然科学基金面上项目、青年基金；省自然科学基金，省科技支撑计划，辽宁省高等学校优秀人才支持计划，省渔业厅项目，教育部、人事部、教育厅优秀青年计划，人事厅择优资助，大连市优秀青年科技人才基金等多项课题。
学术期刊上发表论文共53篇，其中SCI 51篇，国际会议1篇，英文专著1篇。申请生物制药相关专利23项，授权7项。获得省部级自然科学奖二等奖一项，省级市级自然科学论文奖一、二、三等奖若干项。辽宁省“百千万人才工程”千人层次人选，辽宁省农业科技创新团队“食品安全源头保障创新团队”岗位专家等。


在读学生人数
博士4人，硕士5人

毕业学生人数
硕士10人，本科20余人