李 娟 详细介绍
性别 女
职称 教授
最高学历 博士
毕业院校 丹麦奥尔胡斯大学(Aarhus University)
专业领域 动物遗传育种与繁殖，猪胚胎工程
联系方式 办公室：逸夫楼4075，e-mail: juanli@njau.edu.cn
研究方向 胚胎早期发育，重编程机制，体细胞核移植（克隆），胚胎玻璃化冷冻等
参加学术团体
及任职情况 2018.01-现在，南京农业大学动物科技学院，教授；2010.11-2017.12，南京农业大学动物科技学院，副教授；2009-2011，丹麦奥尔胡斯大学农学院，博士后；2006-2009年，丹麦奥尔胡斯大学，基础医学博士；2005-2006年，丹麦农科院科研助理；2003-2006年，浙江大学生命科学学院，生物信息学硕士。现为中国畜牧兽医学会高级会员，国际胚胎移植学会会员（IETS, International Embryo Transfer Society），欧洲胚胎移植协会会员（AETE: European Embryo Transfer Association）。曾应邀为《Cloning and Stem Cells》、《Life Science》、《Veterinary Journal》、《Molecular Biology Reports》、《Theriogenology》等国际刊物审稿。
承担项目 主持项目如下：2017-2020,江苏省重点研发现代农业面上项目；2017-2020，江苏省自然科学基金面上项目；2017-2020，中央高校业务经费；2015年1月——2017年12月，国家自然科学青年基金；2015，“淮上英才”智慧谷专项创新人才;2014年1月 - 2015年7月，江苏省科技厅异种移植重点实验室开放课题；2014 - 2016年，教育部博士点基金（新教师类）；2013年7月 - 2016年7月，江苏省青年基金；2011年5月 – 2014年4月，中国中央高校基础科研业务经费；2012年9月，留学回国人员科技活动项目择优资助项目；参与项目如下：2017-2020，江苏省农业重大新品种创制项目；2012年 – 2015年，国家自然基金面上项目，参与；2011年11月，南京农业大学人才引进经费，主持；2009年9月-2012年8月，丹麦FTP博士后项目
奖励及荣誉 2016，淮安经济技术开发区人才奖励;2012——2013年，南京农业大学动物科技学院“奥格”育人奖
论文论著
JiaL, Zeng Y, Hu Y, Liu J, Yin C, Niu Y, Wang C, Li J, Jia Y, Hong J, Zhao R。Homocysteineimpairs porcine oocyte quality via deregulation of one carbon metabolism andhypermethylation of mitochondrial DNA. Biol Reprod.2018. doi: 10.1093/biolre/ioy238
XuM, Zeng Y, Chi D, Si L, Qu X, Li J.The dynamic pattern of PLIN3 in pig oocytes andcumulus cells during in vitro maturation. Zygote.2018; 26(1): 40-49.
ChiD, Zeng Y, Xu M, Si L, Qu X, Liu H, Li J. LC3-DependentAutophagy in Pig 2-Cell Cloned Embryos Could Influence the Degradation ofMaternal mRNA and the Regulation of Epigenetic Modification. Cell Reprogram. 2017。19(6): 354-362.
TaoC, Li J, Zhang X, Chen B, Chi D, Zeng Y, Niu Y, Wang C, Cheng W, Wu W, Pan Z,Lian J, Liu H, Miao YL. Dynamic Reorganization of NucleosomePositioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes. Stem Cell Reports. 2017; 9(2): 642-653.
TaoC, Li J, Chen B, Chi D, Zeng Y, Liu H. Genome-scale identification of nucleosomeorganization by using 1000 porcine oocytes at different developmental stages. PLoS One. 2017; 12(3): e**.
JiaL, Li J, He B, Jia Y, Niu Y, Wang C, Zhao R. Abnormally activated one-carbon metabolic pathway isassociated with mtDNA hypermethylation and mitochondrial malfunction in theoocytes of polycystic gilt ovaries. Sci Rep.2013; 6:19436.

Niu YJ, Wang CF, Xiong Q, Yang XX, Chi DM, Li PH, Liu HL, Li J*, Huang RH. (2014). Lipid droplet and mitochondrial content and distribution in parthenogenetically activated pig embryos following delipation. Theriogenology, 2015 Jan 1;83(1):131-8.
Li J*, Gao Y, Petkov S, Purup S, Hyttel P, Callesen H. (2014). Passage number of porcine embryonic germ cells affects epigenetic status and blastocyst rate following somatic cell nuclear transfer. Anim Reprod Sci, 147 (1-2): 39-46.
Liu Y*, Ostrup O, Li R, Li J, Vajta G, Kragh PM, Schmidt M, Purup S, Hyttel P, Kl?rke D, Callesen H. (2014). Long-term effect on in vitro cloning efficiency after treatment of somatic cells with Xenopus egg extract in the pig. Reprod Fertil Dev. 26(7): 1017-31.
Li J*, Li R, Villemoes K, Liu Y, Purup S, Callesen H. (2013). Developmental potential and kinetics of pig embryos with different cytoplasmic volume. Zygote, 15:1-11. [Epub ahead of print]
Li J*, Li R, Liu Y, Villemoes K, Purup S, Callesen H. (2013). Developmental Kinetics of pig embryos by parthenogenetic activation or by handmade cloning. Reprod Domestic Anim, 48(5):866-73.
Xiong, Qiang; Fu, Hang; Chen, Jie; Liu, Honglin; Li, Juan* (2013).The effect of 5-aza-2 '-deoxycytidine treatment on the methylation related gene expression in pig. Transgenic Research. 22(1):238-238. (Abstract).
Li J, Pedersen HS, Li R, Adamsen J, Liu Y, Schmidt M, Purup S, Henrik Callesen*. (2014). Developmental potential of pig embryos reconstructed by use of sow versus pre-pubertal gilt oocytes after somatic cell nuclear transfer. Zygote 22(3): 356-365.
Al-Mashhadi RH, S?rensen CB, Kragh PM, Christoffersen C, Mortensen MB, Tolbod LP, Thim T, Du YT, Li J, Liu Y, Moldt B, Schmidt M, Vajta G, Larsen T, Purup S, Bolund L, Nielsen LB, Callesen H, Falk E, Mikkelsen JG, Bentzon JF*. (2012) Familial hypercholesterolemia and atherosclerosis in cloned minipigs created by DNA transposition of a human PCSK9 gain-of-function mutant. Science Translational Medicine. 2013 Jan 2;5(166):166ra1.
Staunstrup NH, Madsen J, Primo MN, Li J, Liu Y, Kragh PM, Li R, Schmidt M, Purup S, Dagn?s-Hansen F, Svensson L, Petersen TK, Callesen H, Bolund L, Mikkelsen JG*. (2012). Development of Transgenic Cloned Pig Models of Skin Inflammation by DNA Transposon-Directed Ectopic Expression of Human β1 and α2 Integrin. PLoS One. 7(5): e36658. Epub 2012 May 10.
Deshmukh RS*, Ostrup O, Strejcek F, Vejlsted M, Lucas-Hahn A, Petersen B, Li J, Callesen H, Niemann H, Hyttel P. (2012). Early Aberrations in Chromatin Dynamics in Embryos Produced Under In Vitro Conditions. Cell Reprogram. 14(3):225-34.
Liu Y*, Ostrup O, Li J, Vajta G, Lin L, Kragh PM, Purup S, Hyttel P, Callesen H. (2012). Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin. Zygote. 20(1): 61-6.
Li R*, Li J, Liu Y, Kragh PM, Hyttel P, Schmidt M, Callesen H. (2012). Optimal developmental stage for vitrification of parthenogenetically activated porcine embryos. Cryobiology. 64(1):60-4.
Liu Y*, ?strup O, Li J, Vajta G, Kragh PM, Purup S, Callesen H. (2011). Cell colony formation induced by Xenopus egg extract as a marker for improvement of cloned blastocyst formation in the pig. Cell Reprogram. 13(6):521-6.
R S. Deshmukh*, O. Ostrup, E. Ostrup, M. Vejlsted, H. Niemann, A. Lucas-Hahn, B. Petersen, J. Li, H. Callesen and P. Hyttel. (2011). DNA methylation in porcine preimplantation embryos developed in-vivo and produced by in-vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer. Epigenetics, 6(2): 177-87.
Schmidt M, Winter KD, Dantzer V, Li J, Kragh PM, Du Y, Lin L, Liu Y, Vajta G, Sangild PT, Callesen H, Agerholm JS*. (2011). Maternal endometrial oedema may increase perinatal mortality of cloned and transgenic piglets. Reprod Fertil Dev. 23(5):645-53.
Jakobsen JE, Li J, Moldt B, Kragh PM, Callesen H, Hertz JM, Bolund L, J?rgensen AL, Mikkelsen JG and Nielsen AL* (2011). Establishment of a porcine fibroblast derived cell line for locus-directed transgene expression in cell cultures and blastocysts. Molecular Biotechnology reports. 38(1): 151-61.
Jakobsen J.E., Li J., Kragh P.M., Moldt B., Lin L., Liu Y., Schmidt M., Winther K.D., Schyth B.D., Holm I.E., Vajta G., Bolund L., Callesen H., J?rgensen A.L., Nielsen A.L., and Mikkelsen J.G.* (2011). Pig transgenesis by sleeping beauty DNA transposition. Transgenic Research, 20(3):533-45.
Luo Y*, Li J, Liu Y, Lin L, Du Y, Li S, Yang H, Vajta G, Callesen H, Bolund L, S?rensen CB. (2011), High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer. Transgenic Res. Transgenic Res. 20(5):975-88.
Schmidt M*, Kragh PM, Li J, Du Y, Lin L, Liu Y, Vajta G, Callesen H. (2010). Pregnancies and piglets from large white sow recipients after two transfer methods of cloned and transgenic embryos of different pig breeds. Theriogenology, 74(7), 1233-40.
Li J*, Svarcova O, Villemoes K, Kragh PM, Schmidt M, B?gh IB, Zhang YH, Du y, Lin L, Purup S, Xue QZH, Bolund L, Yang HM, Maddox-Hyttel P, Vajta G. (2009). High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. Theriogenology, 68(8):1104-10.
Li J*, Villemoes K, Zhang YH, Du Y, Kragh P, Purup S, Xue QZH, Pedersen AM, J?gensen AL, Jakobsen JE, Bolund L, Yang HM, Vajta G. (2009). Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryos. Reproduction in Domestic Animals. 44(1):122-7.
J. Li*, G. Vajta and H. Callesen. (2009). Comparison of cell numbers of Zona-Intact and Zona-Free parthenogentically activated porcine embryos cultured in vitro. Reproduction, Fertility and Development，Vol 22(1): 234. (Abstract)
Kragh PM*, Nielsen AL, Li J, Du Y, Lin L, Schmidt M, B?gh IB, Holm IE, Jakobsen JE, Johansen MG, Purup S, Bolund L, Vajta G, J?rgensen AL. (2009). Hemizygous minipugs produced by random gene insertion and handmade cloning express the Alzheimer’s disease-causing dominant mutation APPsw. Transgenic Research. 18(4): 545-58.
Lin L*, Du Y, Liu Y, Kragh P, Li J, Purup S, Kuwayama M, Zhang X, Yang H.M, Bolund L and Vajta G. (2009). Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes. Reproduction Biomedicine Online: 18(3): 360-6.
Liu Y*, Du Y, Lin L, Li J, Kragh P, Kuwayama M, Bolund L, Yang H.M, and Vajta G. (2008). Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes. Cryo Letters: 29(4):315-20.
Du Y*, Lin L, Schmidt M, B?gh IB, Kragh P, S?rensen CB, Li J, Purup S, Pribenszky C, Moln?r M, Kuwayama M, Zhang X, Yang H.M, Bolund L, Vajta G. (2008). High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning and Stem Cells, 10(3): 325-30.
J. Li*, Y. Du, P.M. Kragh, S. Purup, K. Villemoes, A. M. Pedersen, A. L. J?rgensen, L. Bolund, H.M. Yang and G. Vajta. (2007). Development of pig embryos cloned from donor cells treated with trichostatin A. Reproduction, Fertility and Development. Vol 20(1): 101. (Abstract)
Du Y*, Li J, Kragh P, Zhang Y, Schmidt M, B?gh I.B, Zhang X, Purup S, Kuwayama M, J?rgensen A.L, Pedersen A, Villemoes K, Yang H.M, Bolund L and Vajta G. (2007). Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer. Cloning and Stem Cells: 9(4):469-76.
Zhang Y*, Li J, Villemoes K, Pedersen A, Purup S, Vajta G. (2007). An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer. (2007). Cloning and Stem Cells: 9(3): 357-63.
Du Y*, Kragh P, Zhang Y.H, Li J, Schmidt M, B?gh I.B, Zhang X, Purup S, J?rgensen A.L, Pedersen A, Villemoes K, Yang H.M, Bolund L and Vajta G. (2007). Piglets born from handmade cloning, an innovative cloning method without micromanipulation. Theriogenology, 68(8): 1104-10.
Du Y*, Zhang Y.H, Li J, Kragh P, Kuwayama M, Ieda S, Zhang X, Schmidt M, B?gh I.B, Purup S, Pedersen A, Vilemoes K, Yang H.M, Bolund L and Vajta G. (2007). Simplified cryopreservation of porcine cloned blastocysts. Cryobiology: 54(2): 181-7.
Li J*, Du Y., Zhang YH., Kragh PM., Purup S., Bolund L., Yang H., Xue QZH., Vajta G. (2006). Chemically assisted handmade enucleation of porcine oocytes, Cloning and Stem Cells, 8(4):241-50.
J. Li, Y.H. Zhang, Y. Du, P.M. Kragh, S. Purup, A.M. Pedersen, K. Villemoes, A.L. J?rgensen, L. Boludn, Q.Z. Xue, H.M. Yang, G. Vajta. (2006). Efficiency of two enucleation methods for the production of transgenic pig embryos by handmade cloning. Reproduction, Fertility and Development. Vol 19(1): 148. (Abstract)
J Li, Y. Du, Y.H. Zhang, P.M. Kragh, L. Bolund, H. Yang, Q.Z Xue and G. Vajta. (2006). Efficiency of chemically assisted handmade enucleation of porcine ooctyes. Reproduction in Domestic Animals. Vol 41(4): 340. (Abstract)
杨希祥，李智方，龙川，冯冲，储明星，潘登科，李娟*，（2014）。小型猪克隆胚胎体外培养阶段的优化。中国畜牧兽医，Vol. 41(7): 182-186.
基因表达序列标签（EST）数据分析手册，胡松年主编，浙江大学出版社，2005年，第六章，EST功能分类与代谢途径分析（李娟，刘贵明，薛庆中），p128-149。