Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陶谦教授 (肿瘤学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/ijc.25443 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/23WOS source URL
其它资讯
摘要Nasopharyngeal carcinoma (NPC) is a rare malignancy with unique genetic, viral and environmental characteristic that distinguishes it from other head and neck carcinomas. The clinical management of NPC remains challenging largely due to the lack of early detection strategies for this tumor. In our study, we have sought to identify novel genes involved in the pathogenesis of NPC that might provide insight into this tumor's biology and could potentially be used as biomarkers. To identify these genes, we studied the epigenetics of NPC by characterizing a panel of methylation markers. Eighteen genes were evaluated by quantitative methylation-specific polymerase chain reaction (PCR) in cell lines as well as in tissue samples including 50 NPC tumors and 28 benign nasopharyngeal biopsies. Significance was evaluated using Fisher's exact test and quantitative values were optimized using cut off values derived from receiver-operator characteristic curves. The methylation status of AIM1, APC, CALCA, deleted in colorectal carcinomas (DCC), DLEC, deleted in liver cancer 1 (DLC1), estrogen receptor alpha (ESR), FHIT, KIF1A and PGP9.5 was significantly associated with NPC compared to controls. The sensitivity of the individual genes ranged from 26 to 66% and the specificity was above 92% for all genes except FHIT. The combination of PGP9.5, KIF1A and DLEC had a sensitivity of 84% and a specificity of 92%. Ectopic expression of DCC and DLC1 lead to decrease in colony formation and invasion properties. Our results indicate that methylation of novel biomarkers in NPC could be used to enhance early detection approaches. Additionally, our functional studies reveal previously unknown tumor suppressor roles in NPC.
着者Loyo M, Brait M, Kim MS, Ostrow KL, Jie CFC, Chuang AY, Califano JA, Liegeois NJ, Begum S, Westra WH, Hoque MO, Tao QA, Sidransky D
期刊名称INTERNATIONAL JOURNAL OF CANCER
详细描述[Epub ahead of print].
出版年份2011
月份3
日期15
卷号128
期次6
出版社JOHN WILEY & SONS INC
页次1393 - 1403
国际标準期刊号0020-7136
电子国际标準期刊号1097-0215
语言英式英语
关键词biomarker; dna methylation; nasopharyngeal carcinoma
Web of Science 学科类别Oncology; ONCOLOGY
