Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 庄立信教授 (肿瘤学系) |
| 杨明明教授 (肿瘤学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1046/j.1365-2893.1999.00154.x |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/31WOS source URL
其它资讯
摘要Hepatitis B virus (HBV) envelope mutants in the region encoding the highly immunogenic major hydrophilic region (MHR) of surface antigen (HBsAg) have been associated with vaccine failure and chronic infection, To determine if these mutants are associated with the development of human hepatocellular carcinoma (HCC), we measured the frequency and nature of such mutants in 23 HBV-associated HCC and various control tissues by performing Southern blot analysis, the polymerase chain reaction (PCR) and direct sequencing, The HBV genome was present mainly in an integrated form and, in most of the samples, the envelope gene was intact. Amino acid substitutions, involving the MHR region in the HCC tissues, were analysed in 11 (61.1%) of 18 patients with HCC. The mutation Gly145Arg, which has been reported to be associated with immunoevasion, was found in seven of the 18 HCC tissues. A significantly higher frequency of mutations was found in RCC tissues (11 of 18) than in the corresponding non-tumorous tissue of the same patients (one of eight), and in samples from patients with acute (one of 19) or chronic (three of http://aims.cuhk.edu.hk/converis/portal/Publication/31) HBV infection (P < 0.001, Fisher's exact test). The accumulation of these envelope mutants in the HCC tissue suggests that such envelope protein mutations may play a role in the process of oncogenesis and that specific vaccines may need to be developed to prevent the occurrence of mutant HBV-associated HCC, Alternatively, the progressive accumulation of mutants in patients with acute hepatitis, chronic hepatitis and HCC may reflect the increased length of duration of HBV infection in these groups of liver lesions.
着者Zhong S, Chan JYH, Yeo W, Tam JS, Johnson PJ
期刊名称Journal of Viral Hepatitis
出版年份1999
月份5
日期1
卷号6
期次3
出版社BLACKWELL SCIENCE LTD
页次195 - 202
国际标準期刊号1352-0504
电子国际标準期刊号1365-2893
语言英式英语
关键词envelope protein mutant; HBsAg; hepatitis B virus; hepatocellular carcinoma
Web of Science 学科类别Gastroenterology & Hepatology; GASTROENTEROLOGY & HEPATOLOGY; Infectious Diseases; INFECTIOUS DISEASES; Virology; VIROLOGY
