Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 赵海潞博士 (内科及药物治疗学系) |
| 陈重娥教授 (内科及药物治疗学系) |
| 唐俊业教授 (内科及药物治疗学系) |
| 黎永祥教授 (病理解剖及细胞学系) |
| 包立怡教授 (药剂学院) |
| 隋昳博士 (内科及药物治疗学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.trsl.2008.10.009 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/19WOS source URL
其它资讯
摘要The amyloid hypothesis of type 2 diabetes mellitus postulates that elevated levels of normally expressed monomeric proteins of human islet amyloid polypeptide (NAPP) trigger oligomerization that independently causes fibril formation and disease progression. The aim of this study was to demonstrate the existence of amyloid oligomers in human pancreatic islets. Human pancreas tissues were obtained at autopsy of 8 nondiabetic control subjects (mean age = 75.8 +/- 11.7 years, 4 males), 8 type 2 diabetic cases without islet amyloid (mean age = 78.8 +/- 8.5 years, 4 males), and 8 type 2 diabetic patients with islet amyloid (mean age = 73.7 +/- 14.2 years, 4 males). Several markers for insulin, IAPP, amyloid fibrils (thioflavin T), and apoptosis (cleaved caspase-3) were used in combination with an oligomer-specific antibody. Two distinct forms of oligomers were found in pancreatic islets. Small spherical puncta were found in approximately 3% to 20% of the islet cells of nondiabetic subjects, and large curvilinear structures as extracellular oligomers were identified frequently in diabetic islets. Large oligomers were spatially localized adjacent to amyloid fibrils and were associated with apoptosis. This report demonstrates the presence of 2 morphologic classes of amyloid oligomers in human pancreatic islets. The observations warrant function studies to investigate the clinical implications of the amyloid oligomerization in the pathogenesis of type 2 diabetes. (Translational Research 2009; 153:24-32)
着者Zhao HL, Sui Y, Guan J, He L, Gu XM, Wong HK, Baum L, Lai FMM, Tong PCY, Chan JCN
期刊名称TRANSLATIONAL RESEARCH
出版年份2009
月份1
日期1
卷号153
期次1
出版社Elsevier
页次24 - 32
国际标準期刊号http://aims.cuhk.edu.hk/converis/portal/Publication/1931-5244
电子国际标準期刊号1878-1810
语言英式英语
Web of Science 学科类别General & Internal Medicine; Medical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY; Medicine, General & Internal; MEDICINE, GENERAL & INTERNAL; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine
