Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 陈基湘教授 (微生物学系) |
| 许明媚教授 (微生物学系) |
| 陈维智博士 (药剂学院) |
| 叶碧瑶教授 (微生物学系) |
| 杨仲敏女士 (微生物学系) |
| 林伟业博士 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1128/JCM.00280-07 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/69WOS source URL
其它资讯
摘要Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 different respiratory pathogens: influenza A virus (H1N1, H3N2, and 115N1); influenza B virus; parainfluenza virus types 1, 2, 3, 4a, and 4b; respiratory syncytial virus A and B; human rhinoviruses; human enteroviruses; human coronaviruses OC43 and 229E; severe acute respiratory syndrome coronavirus; human metapneumoviruses; Mycoplasma pneumoniae; Chlamydophila pneumoniae; Legionella pneumophila; and adenoviruses (A to F). These multiplex nested PCRs adopted fast PCR technology. The high speed of fast PCR (within 35 min) greatly improved the efficiency of these assays. The results show that these multiplex nested PCR assays are specific and more sensitive (100- to 1,000-fold) than conventional methods. Among the 303 clinical specimens tested, the multiplex nested PCR achieved an overall positive rate of 48.5% (95% confidence interval [CI], 42.9 to 54.1%), which was significantly higher than that of virus isolation (20.1% [95% CI, 15.6 to 24.6%]) and that of direct detection by immunofluorescence assay (13.5% [95% CI, 9.7 to 17.4%]). The improved sensitivity was partly due to the higher sensitivity of multiplex nested PCR than that of conventional methods in detecting cultivatable viruses. Moreover, the ability of the multiplex nested PCR to detect noncultivatable viruses, particularly rhinoviruses, coronavirus OC43, and metapneumoviruses, contributed a major gain (15.6%) in the overall positive rate. In conclusion, rapid multiplex nested PCR assays can improve the diagnostic yield for respiratory infections to allow prompt interventive actions to be taken.
着者Lam WY, Yeung ACM, Tang JLW, Ip M, Chan EWC, Hui M, Chan PKS
期刊名称Journal of Clinical Microbiology
出版年份2007
月份11
日期1
卷号45
期次11
出版社AMER SOC MICROBIOLOGY
页次3631 - 3640
国际标準期刊号0095-1137
电子国际标準期刊号1098-660X
语言英式英语
Web of Science 学科类别Microbiology; MICROBIOLOGY
