Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 左中教授 (药剂学院) |
| 林革教授 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.lfs.2005.10.038 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/27WOS source URL
其它资讯
摘要Extensive intestinal glucuronidation has been previously reported in both human and animals after oral administration of naturally occurred flavonoids. The present study aims to investigate the relationship between human intestinal glucuronidation activity and the position of hydroxyl substitution on flavonoids. Seven commercially available mono-hydroxyflavones (HF), namely 3-, 5-, 6-, 7-, 2'-, 3'- and 4'-monohydroxyflavones, were chosen as model compounds. Glucuronidation activity of the selected seven HFs was investigated by incubating each HF at various concentrations with human jejunum S9 at 37 degrees C for 10 min. The generated glucuronides were identified by HPLC/MS and quantified by HPLC/UV. Metabolic kinetics parameters including K-m and V-max of each HF were determined. The results demonstrated that the glucuronidation activity of 6- and 3'-mono-hydroxyflavones was much greater than that of 3-, 4-, 7- and 2-HF with 5-HF to be the lowest. The findings imply that nucleophilicity and stereo-conformation of OH substituents are crucial for the intestinal glucuronidation of flavonoids. (c) 2005 Elsevier Inc. All rights reserved.
着者Zhang L, Lin G, Zuo Z
期刊名称LIFE SCIENCES
出版年份2006
月份5
日期8
卷号78
期次24
出版社PERGAMON-ELSEVIER SCIENCE LTD
页次http://aims.cuhk.edu.hk/converis/portal/Publication/2772 - http://aims.cuhk.edu.hk/converis/portal/Publication/2780
国际标準期刊号0024-3205
电子国际标準期刊号1879-0631
语言英式英语
关键词glucuronidation; human intestine; mono-hydroxyflavones
Web of Science 学科类别Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine
