Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
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| 数位物件识别号 (DOI) ○○@http://aims.cuhk.edu.hk/converis/portal/Publication/2$@○○ 出版商提供的全文 ○○@http://aims.cuhk.edu.hk/converis/portal/Publication/3$@○○ |
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Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/2Scopus source URL
其它资讯
摘要The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the M1http://aims.cuhk.edu.hk/converis/portal/Publication/3 strain (such as an amber mutation, or an EcoK or EcoB site) which allows selection for the progeny of M1http://aims.cuhk.edu.hk/converis/portal/Publication/3 replication derived from the minus (mutagenized) strand. Several mutations have been constructed in the ATP binding site to elucidate the roles of individual residues in catalysis and substrate binding and it has even been possible to construct mutants which have improved affinity for ATP. Mutations in various surface lysine and arginine residues have allowed us to identify potential contacts with the tRNA, and indicate that a cluster of basic residues close to the C-terminus of the enzyme probably makes important interactions with the tRNA.
出版社接受日期17.04.1985
着者Hugues Bedouelle, Paul Carter, Mary M.Y. Waye, Greg Winter, Denise M. Lowe, Anthony J. Wilkinson, Alan R. Fersht
期刊名称Biochimie
出版年份1985
卷号67
期次7-8
出版社Elsevier
页次7http://aims.cuhk.edu.hk/converis/portal/Publication/37 - 74http://aims.cuhk.edu.hk/converis/portal/Publication/3
国际标準期刊号0http://aims.cuhk.edu.hk/converis/portal/Publication/300-9084
电子国际标準期刊号16http://aims.cuhk.edu.hk/converis/portal/Publication/38-618http://aims.cuhk.edu.hk/converis/portal/Publication/3
语言英式英语
关键词genetic engineering, site-directed mutagenesis, tRNA synthetase, specificity
