Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 胡嘉麒教授 (麻醉及深切治疗学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1371/journal.pone.0046565 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/6Scopus source URL
其它资讯
摘要Most cases of congenital spinal deformities were sporadic and without strong evidence of heritability. The etiology of congenital spinal deformities is still elusive and assumed to be multi-factorial. The current study seeks to elucidate the effect of maternal vitamin A deficiency and the production of congenital spinal deformities in the offsping. Thirty two female rats were randomized into two groups: control group, which was fed a normal diet; vitamin A deficient group, which were given vitamin A-deficient diet from at least 2 weeks before mating till delivery. Three random neonatal rats from each group were killed the next day of parturition. Female rats were fed an AIN-9http://aims.cuhk.edu.hk/converis/portal/Publication/3G diet sufficient in vitamin A to feed the rest of neonates for two weeks until euthanasia. Serum levels of vitamin A were assessed in the adult and filial rats. Anteroposterior (AP) spine radiographs were obtained at week 2 after delivery to evaluate the presence of the skeletal abnormalities especially of spinal deformities. Liver and vertebral body expression of retinaldehyde dehydrogenase (RALDHs) and RARs mRNA was assessed by reverse transcription-real time PCR. VAD neonates displayed many skeletal malformations in the cervical, thoracic, the pelvic and sacral and limbs regions. The incidence of congenital scoliosis was 1http://aims.cuhk.edu.hk/converis/portal/Publication/3.79% (8/58) in the filial rats of vitamin A deficiency group and 0% in the control group. Furthermore, vitamin A deficiency negatively regulate the liver and verterbral body mRNA levels of RALDH1, RALDH2, RALDHhttp://aims.cuhk.edu.hk/converis/portal/Publication/3, RAR-α, RAR-β and RAR-γ. Vitamin A deficiency in pregnancy may induce congenital spinal deformities in the postnatal rats. The decreases of RALDHs and RARs mRNA expression induced by vitamin A deprivation suggest that vertebral birth defects may be caused by a defect in RA signaling pathway during somitogenesis. ? 2012 Li et al.
着者Li Z., Shen J., Wu W.K.K., Wang X., Liang J., Qiu G., Liu J.
期刊名称PLoS ONE
详细描述To ORKTS: URL fm P124http://aims.cuhk.edu.hk/converis/portal/Publication/640 record.
出版年份2012
月份10
日期5
卷号7
期次10
出版社Public Library of Science
出版地United States
国际标準期刊号19http://aims.cuhk.edu.hk/converis/portal/Publication/32-http://aims.cuhk.edu.hk/converis/portal/Publication/620http://aims.cuhk.edu.hk/converis/portal/Publication/3
语言英式英语
