Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 袁平教授 (李嘉诚健康科学研究所) |
| 袁平教授 (李嘉诚健康科学研究所) |
| 黄志超教授 (妇产科学系) |
| 黄振国教授 (化学病理学系) |
| 麦经纶教授 (生物医学学院) |
| 陈颖博士 (李嘉诚健康科学研究所) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1371/journal.pone.0079867 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
其它资讯
摘要The Hippo pathway is an evolutionary conserved pathway that involves cell proliferation, differentiation, apoptosis and organ size regulation. Mst1 and Mst2 are central components of this pathway that are essential for embryonic development, though their role in controlling embryonic stem cells (ES cells) has yet to be exploited. To further understand the Mst1/Mst2 function in ES cell pluripotency and differentiation, we derived Mst1/Mst2 double knockout (Mst-/-) ES cells to completely perturb Hippo signaling. We found that Mst-/- ES cells express higher level of Nanog than wild type ES cells and show differentiation resistance after LIF withdrawal. They also proliferate faster than wild type ES cells. Although Mst-/- ES cells can form embryoid bodies (EBs), their differentiation into tissues of three germ layers is distorted. Intriguingly, Mst-/- ES cells are unable to form teratoma. Mst-/- ES cells can differentiate into mesoderm lineage, but further differentiation to cardiac lineage cells is significantly affected. Microarray analysis revealed that ligands of non-canonical Wnt signaling, which is critical for cardiac progenitor specification, are significantly repressed in Mst-/- EBs. Taken together our results showed that Mst1/Mst2 are required for proper cardiac lineage cell development and teratoma formation.
着者Li P, Chen Y, Mak KK, Wong CK, Wang CC, Yuan P
期刊名称PLoS ONE
详细描述To ORKTS: Duplicate record P1http://aims.cuhk.edu.hk/converis/portal/Publication/3756http://aims.cuhk.edu.hk/converis/portal/Publication/3 deleted.
出版年份201http://aims.cuhk.edu.hk/converis/portal/Publication/3
月份11
日期5
卷号8
期次11
出版社PUBLIC LIBRARY SCIENCE
国际标準期刊号19http://aims.cuhk.edu.hk/converis/portal/Publication/32-620http://aims.cuhk.edu.hk/converis/portal/Publication/3
语言英式英语
Web of Science 学科类别Multidisciplinary Sciences; MULTIDISCIPLINARY SCIENCES; Science & Technology - Other Topics
