Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 林伟基教授 (化学病理学系) |
| 林以安小姐 (化学病理学系) |
| 刘建盟博士 (病理解剖及细胞学系) |
| 黄振国教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.imbio.2012.05.019 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/13WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/14Scopus source URL
其它资讯
摘要Eosinophils are the principal effector cells of allergic inflammation, and hematopoietic cytokine granulocyte macrophage colony-stimulating factor (GM-CSF) is the primary cytokine that activates and prolongs the survival of eosinophils in local inflammatory sites by mediating anti-apoptotic activity in allergic inflammation. To investigate the immunopathological role of microRNA (miRNA) in allergic inflammation, we elucidated the regulatory mechanisms of miRNA on the GM-CSF-mediated in vitro survival in eosinophils. Eosinophils were purified from fresh human peripheral blood buffy coat fraction obtained from adult volunteer using microbead magnetic cell sorting. The apoptosis, viability and phosphorylation of extracellular signal-regulated kinase (ERK) were assessed by flow cytometry, and the expression of miRNA was analyzed using Agilent Human miRNA Microarray with Human miRNA Microarray Version 3 and real time RT-PCR. We have confirmed the increased in vitro viability of GM-CSF-treated eosinophils and upregulated expression of miRNA-21* (miR-21*), a complementary miRNA of miR-21, in GM-CSF-treated eosinophils. The transfection of pre-miR miR-21* precursor molecule could up-regulate the miR-21* expression, subsequently enhance the GM-CSF-activated ERK pathway and reverse the apoptosis of eosinophils, while anti-miR-21* inhibitor could down-regulate the miR-21* expression, suppress the GM-CSF-activated ERK pathway and enhance the apoptosis. Our results should shed light on the potential immunopathological role of miRNA-21* regulating the in vitro apoptosis of eosinophils and development of novel molecular treatment of allergic inflammation. ? 2012 Elsevier GmbH.
着者Wong C.K., Lau K.M., Chan I.H.S., Hu S., Lam Y.Y.O., Choi A.O.K., Lam C.W.K.
期刊名称IMMUNOBIOLOGY
出版年份20http://aims.cuhk.edu.hk/converis/portal/Publication/13
月份2
日期1
卷号218
期次2
出版社Elsevier BV
出版地Netherlands
页次255 - 262
国际标準期刊号0171-2985
语言英式英语
关键词Apoptosis, Eosinophils, Granulocyte macrophage colony-stimulating factor, MicroRNA, Signal transduction
