Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 何浩培教授 (电子工程学系) |
| 黄振国教授 (化学病理学系) |
| 关耀华教授 (生物医学学院) |
| 周俊强先生 (电子工程学系) |
| 江绍佳教授 (生命科学学院) |
| 陈秋兰博士 (电子工程学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.talanta.2012.03.061 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/6WOS source URL
其它资讯
摘要We have utilized various valving scheme to leverage purely rotation-regulated flow control to enable comprehensive cell-based bioassays (CBBs) on centrifuge-based lab-on-a-disc (LOAD). A LOAD has been developed to examine allergic degranulation from live basophils for allergens screening for the first time, which can also be adjusted to suit a wide range of CBBs. In this system, controlled allergic reaction together with mediator separation from basophils using siphon valving and centrifugal sedimentation are realized inside microstructured network. The entire degranulation analysis process including on-demand release of samples, reaction and degranulation, allergic mediator separation and detection is executed in an automatic sequence within a single run. To validate our cell-based approach, detection of degranulation mediated by known secretagagues, ionomycin or chemotatic peptide formyl-methionine-leucine-pheylalanine (fMLP), is first demonstrated. Further experiments using real allergens house dust mite protein (Der p1) and its corresponding human serum IgE also show positive results. The overall efficiency of the assay is 80.http://aims.cuhk.edu.hk/converis/portal/Publication/6%, which is comparable to other conventional methods. With 4 identical units on a disc running in a parallel format, the device offers the possibility of single-step, multiplexed allergens screening. The device is capable of reporting a result within 30 min. It has many desirable merits including fast and multiplexed analysis, low cost, single-step operation, minimal sample volume, less discomfort and most importantly increased safety as patients are no longer susceptible to possible anaphylactic shock reactions induced by the common skin-prick-test. The flexibility of the flow control within the device makes it suitable to a wide range of CBBs. (C) 2012 Elsevier B.V. All rights reserved.
着者Chen QL, Cheung KL, Kong SK, Zhou JQ, Kwan YW, Wong CK, Ho HP
期刊名称Talanta
出版年份2012
月份8
日期15
卷号97
出版社Elsevier
页次48 - 54
国际标準期刊号0039-9140
电子国际标準期刊号1873-3573
语言英式英语
关键词Allergen screening; Cell-based bioassays; Centrifuge; Lab-on-a-disc
Web of Science 学科类别Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL
