Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 崔耀隆教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1089/cbr.2012.1227 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/5WOS source URL
其它资讯
摘要Reprogramming of cancer cells into induced pluripotent stem cells (iPSCs) opens up the possibility of converting malignant cells into any cell type, including those best suited to be developed as cancer vaccines. Mouse models are needed to evaluate and optimize the therapeutic efficacy of such novel cancer vaccines. However, only human cancer cell lines have been reported as being reprogrammed into iPSCs. Here, we report a proof-of-principle study which shows that mouse cancer cells can be reprogrammed into iPSCs that are capable of subsequent differentiation. Four canonical reprogramming transcription factors, Oct3/4, Sox2, Klf4, and c-Myc, were introduced by plasmid transfection into mouse Lewis lung carcinoma D122 harboring Nanog-GFP reporter. Green fluorescent cells were found clustered into embryonic stem cell (ESC)-like colonies expressing ESC markers, Oct4 and SSEA-1. Bisulfite genomic sequencing analyses of these cells revealed hypomethylation of the Nanog promoter. The expression of a host of pluripotency genes by these reprogrammed cells at levels similar to those of ESCs was confirmed by quantitative real-time PCR. Functional pluripotency of the reprogrammed cells was demonstrated by their ability to form embryoid bodies and differentiate into neuronal progenitors on retinoic acid treatment. This study indicates the feasibility of developing iPSC-based experimental cancer vaccines for immunotherapy in mouse models.
着者Lin FKY, Chui YL
期刊名称CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
出版年份2012
月份12
日期1
卷号27
期次10
出版社Mary Ann Liebert
页次694 - 700
国际标準期刊号1084-978http://aims.cuhk.edu.hk/converis/portal/Publication/5
语言英式英语
关键词induced pluripotent stem cells; mouse Lewis lung carcinoma; reprogramming cancer cells
Web of Science 学科类别Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Oncology; ONCOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Radiology, Nuclear Medicine & Medical Imaging; RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING; Research & Experimental Medicine
