申志发，加拿大蒙特利尔大学博士、研究员，NIH博后，新乡医学院教授，博士生导师。研究方向：肿瘤转移分子机制与靶向治疗。在该领域，应用活细胞单分子成像、核酸适配体筛选等核心技术，取得了多项具有国际先进水平，甚至有望在个别领域具有原创性成果。近五年研究成果先后以第一作者或通讯在ANGEW CHEM INT EDIT、Nature Communications、Genes and Development、Molecular Biology of the Cell, Anal. Chem.等知名学术期刊上发表。


近五年论文

1. Huo Xu; Yafeng Zhang; Shuxin Zhang; Changhe Ouyang; Feng Li; Suhong Yu; Xiaofang Geng,Zhifa Shen*, Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene. Sensors & Actuators: B. Chemical. 2019, 281,1016-1022. (JCR一区)
2. Lisha Wang, Ying Han, Shuai Xiao, Sha Lv, Cong Wang, Nan Zhang, Zhengyong Wang,Yongqiong Tang, Hongbo Li, Jianxin Lyu, Huo Xu,Zhifa Shen*. Reverse strand-displacement amplification strategy for rapid detection of p53 gene. Talanta 2018. 187,365-369 . (JCR二区)
3. Zhihua Gao, Chengwei Wu, Sha Lv, Cong Wang, Nan Zhang, Shuai Xiao, Ying Han, Huo Xu, Yan Zhang, Feng Li, Jianxin Lyu, Zhifa Shen*. Nicking-enhanced rolling circle amplification for sensitive fluorescent detection of cancer-related microRNAs. Analytical and Bioanalytical Chemistry 2018. 18, 1277-82 (JCR二区)
4. Zhifa Shen, Feng Li, Yifan Jiang, Chang Chen, Huo Xu, Congcong Li, Zhe Yang, Zaisheng Wu. Palindromic molecule beacon-based cascade amplification for colorimetric detection of cancer genes.Anal. Chem. 2018, 90, 3335-3340. DOI: 10.1021/acs.analchem. 7b04895（JCR一区）
5. Zhengyong Wang; Feng Li; Yan Zhang; Hui Zhao; Huo Xu; Zai-Sheng Wu; Jian-Xin Lyu;Zhi-Fa Shen*.
Sensitive detection of cancer gene based on a nicking-mediated RCA of circular DNA nanomachine.Sensors & Actuators: B. Chemical. 2017, 251: 692-698 doi:10.1016/j.snb.2017.05.061（JCR一区）

6. Dengyou Liu; Qimei Luo; Fawen Deng ; Zhen Li;Benxiang li;Zhifa Shen*.Ultrasensitive electrochemical biosensor based on the oligonucleotide self-assembled monolayer-mediated immunosensing interface.Analytica Chimica Acta. 2017, 971: 26-32 ,（JCR一区）
7. Feng Li, Hui Zhao, Zai-Sheng Wu, Zhe Yang, Huo Xu, Chang Xue,, Jian-xin lyu, Zhi-Fa Shen* Single palindromic molecular beacon-based amplification for genetic analysis of cancers.Biosens Bioelectron. 2017; 91:692-698. doi: 10.1016/j.bios.2017.01.035. （JCR一区）
8. Xu H, Zhang R, Li F, Zhou Y, Peng T, Wang X,Shen Z* Double-hairpin molecular-beacon-based amplification detection for gene diagnosis linked to cancer.Anal Bioanal Chem. 2016 Sep; 408(22):6181-8.
9. Li F, Zhou YY, Peng T, Xu H, Zhang RB, Zhao H, Wang ZY, Lyu JX, Wu ZS,Shen ZF*. Highly sensitive detection of cancer-related genes based on complete fluorescence restoration of a molecular beacon with a functional overhang. Analyst. 2016 Jul 21;141(14):4417-23. （JCR二区）
10. Liu YH, Jin JL, Wang YZ, Tan Y, Zhou YY, Peng T, Li F, Liang WD, Chartrand P, Jiang YY, Shen ZF*. Protrusion-localized STAT3 mRNA promotes metastasis of highly metastatic hepatocellular carcinoma cells in vitro. Acta Pharmacol Sin. 2016 Jun; 37(6):805-13.（JCR二区）
11. Zai-sheng Wu+,Zhifa Shen+, Kha Tram, Bruno J. Salena, and Yingfu Li*Topological DNA Assemblies Containing Identical or Fraternal Twins.ChemBioChem. 2016 Jun 16; 17(12):1142-5. doi: 10.1002/cbic.. (+ These authors contributed equally to this work)（JCR二区）
12. Zhifa Shen,Zai-Sheng Wu, Dingran Chang, Wenqing Zhang, Christine Lee, Peter Kim, Bruno Salena, & Yingfu Li. A Catalytic DNA Activated by a Specific Strain of Bacterial Pathogen. Angew Chem Int Ed Engl. 2016, 55 (7): 2431-4. (Hot Paper DOI: 10.1002/anie.)（JCR一区）
13. Zai-Sheng Wu1^?,Zhifa Shen1^?, Kha Tram, Yingfu Li. Engineering Interlocking DNA Rings with Minimal Physical Interactions.Nature Communications. 2014, 5:4279 (⁺ These authors contributed equally to this work)（JCR一区）
14. Zhifa Shen, Anik St-Denis and Pascal Chartrand. Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to yeast bud.Genes and Development, 2010, 24:1914-1926 （JCR一区）
15. Zhifa Shen,Nicolas Paquin1, Amélie Forget and Pascal Chartrand. Nuclear shuttling of She2p couplesASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.Mol.Bio.Cell, 2009. 20, 2265-2275 . （JCR二区）
主持的科研项目
1 .浙江省自然科学基金面上项目，LY16C070002，HCCLM3细胞突出中STAT3 mRNA分子内定位元件的分析与鉴定，2016/01-2018/12，10万元，主持。
2.新乡医学院人才启动基金，QTJ15005，肝癌细胞突出中STAT3mRNA定位机制，2017/09-2022/08，300万元，主持。
3. Mechanisms regulating mRNA localization and translational control. Canadian Institutes of Health Research (CIHR), MOP130325 2014/04-2017/03，200万元（40万加拿大元）、主持。
4． 省部共建重点项目，突出中STAT3 mRNA定位机制与肝癌转移， WKJ-ZJ-1723 2017-2020，主持，30万元。
1. 浙江省自然科学基金面上项目，识别慢性粒细胞白血病DNA分子探针的筛选与应用研究，LY17H200002，2017-2019，项目负责人，10万元
2. 温州市重大科技专项项目，微环境下细胞突出RNA分子动态交流与肝癌转移，2016Y0852，2017-2020，主持，100万元