姓名： 李用芳
职称： 副教授/硕导师
办公电话：

电子邮箱： 041031@htu.edu.cn








个人简介：
河南师大生科院学士、硕士，以色列本古里安大学博士，美国俄克拉荷马州立大学博士后及研究助理教授（2008-2015）。在Trends Plant Sci.， Plant J., Nucleic Acids Res., New Phytol., RNA等期刊上发表Sci期刊论文20余篇，其中高被引论文2篇，单篇论文引用次数达339次，216次。同时为Springer出版的五部学术专著撰写5章专题。现主持国家自然科学基金面上基金一项

研究领域：
小RNA与植物的生长发育和胁迫应答
转录后调控机理

主要学术及社会兼职：
中国生物化学与分子生物学学会河南省分会副理事长
主持或参加科研项目情况：
1.MicroRNA介导的转录后调控在冬小麦春化过程中的作用机理研究，国家自然科学基金面上基金。主持, 2018-2021。
2.GhACR1调控棉花耐盐的机理研究，国家自然科学基金，参与，2017-2019.
3.Building Oklahoma's leadership in cellulosicbioenergy. 美国国家自然科学基金NSF-EPSCoR. 参与,2008-2013。
4.Improving abiotic stress tolerance byengineering microRNA398 resistant superoxide dismutases in Rice and Tomato. 美国农业部USDA-NRI, 参与，2008-2011。
5.Nanocarrier-mediated Targeting ofBioscavengers to the Red Blood Cell for Prolonged circlulation，美国国防部 DTRA项目, 参与,2013-2017。
6.Role of Arabidopsis RNA binding proteins instress signaling。Oklahoma Center forAdvancement of Science and Technology，参与，2012-2014.

学术成果：
代表性论文：
1. LiYF, Zheng Y, Addo-Quaye C, Zhang L, Saini A, Jagadeeswaran G, Axtell MJ,Zhang W and Sunkar R (2010) Transcriptome-wide identification of microRNAtargets in rice. Plant J. 62(5): 742-759 (SCI TOP期刊，高被引论文,引用261次)
2. Sunkar R, Li YF, Jagadeeswaran G (2012). Functions of microRNAs in plantstress responses. Trends Plant Sci. 17(4):196-203 (SCI TOP期刊，高被引论文, 引用480次)
3. Zheng Y, Li YF*, Sunkar R & Zhang W (2013). SeqTar: An Effective Methodfor Identifying MicroRNA Guided Cleavage Sites from Degradome of PolyadenylatedTranscripts in Plants. Nucleic Acids Research. 40(4):e28 (SCI TOP期刊.*: 共同第一)
4. Li, YF., Zhao, M., Wang, M., Guo, J., Wang, L., Ji, J., Qiu,Z., Zheng, Y., and Sunkar, R. (2019). An improved method of constructingdegradome library suitable for sequencing using Illumina platform. PlantMethods 15.
5. Li, YF., Wei, K., Wang, M., Wang, L., Cui, J., Zhang, D., Guo,J., Zhao, M., and Zheng, Y. (2019). Identification and Temporal ExpressionAnalysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grownin the Field. Front Genet 10, 779.
6. Li, YF., Zheng, Y.,Vemireddy, L.R., Panda, S.K., Jose, S., Ranjan, A., Panda, P., Govindan, G.,Cui, J., Wei, K., Yaish, M.W., Naidoo, G.C., and Sunkar, R. (2018). Comparativetranscriptome and translatome analysis in contrasting rice genotypes revealsdifferential mRNA translation in salt-tolerant Pokkali under salt stress. BMCGenomics 19, 935.
7. Wang, L., Wang, L., Chang, W., Li,Y., and Wang, L. (2019). MicroRNA-373 promotes the development ofesophageal squamous cell carcinoma by targeting LATS2 and OXR1. Int J BiolMarkers 34, 148-155 （通讯作者）.
8. Chen, K., Liu, L., Zhang, X., Yuan, Y., Ren, S., Guo, J., Wang, Q.,Liao, P., Li, S., Cui, X., Li, Y.F.,and Zheng, Y. (2018). Phased secondary small interfering RNAs inPanaxnotoginseng. BMC Genomics 19, 41. （通讯作者）
9. LiYF, Wang Y, Kakani G& Mahalingam R (2013). Transcriptome Analysis ofHeat Stress Response In Switchgrass (Panicum Virgatum L.) BMC Plant Biol. 2013Oct 6; 13(1):153. doi: 10.1186/1471-2229-13-153.
10. LiYF, Zheng Y, Jagadeeswaran G & Sunkar R (2013). Characterization ofsmall RNAs and their target genes in wheat seedlings using sequencing-basedapproaches. Plant Science. 203-204: 17-24.
11. Jagadeeswaran G, Li YF, Sunkar R (2014). Redox signaling mediates the expression ofa sulfate-deprivation-inducible microRNA395 in Arabidopsis. Plant J. 2014Jan;77(1):85-96. doi: 10.1111/tpj.12364 （SCI TOP期刊）
12. Jagadeeswaran G, Li YF, Sunkar R (2014). Redox signaling mediates the expression ofa sulfate-deprivation-inducible microRNA395 in Arabidopsis. Plant J. 2014Jan;77(1):85-96. doi: 10.1111/tpj.12364 （SCI TOP期刊）
13. Zheng, Y., Chen, K., Xu, Z., Liao, P., Zhang, X., Liu, L., Wei, K.,Liu, D., Li, Y.F., Sunkar, R., andCui, X. (2017). Small RNA profiles from Panax notoginseng roots differing insizes reveal correlation between miR156 abundances and root biomass levels. SciRep 7, 9418.
14.Jagadeeswaran, G., Zheng, Y., Li, Y.F., Shukla, L.I., Matts, J., Hoyt, P., Macmil, S.L., Wiley,G.B., Roe, B.A., Zhang, W., and Sunkar, R. (2009). Cloning and characterizationof small RNAs from Medicago truncatula reveals four novel legume-specificmicroRNA families. New Phytol 184, 85-98.
15. Addo-Quaye, C., Snyder, J.A.,Park, Y.B., Li, Y.F., Sunkar, R.,and Axtell, M.J. (2009). Sliced microRNA targets and precise loop-firstprocessing of MIR319 hairpins revealed by analysis of the Physcomitrella patensdegradome. RNA 15, 2112-2121.
16. Li, X., Ding, X., Li, Y., Wang, L., and Fan, J. (2016).A TiS2 nanosheet enhanced fluorescence polarization biosensor for ultra-sensitivedetection of biomolecules. Nanoscale 8, 9852-9860.
专著章节
Li YF, Mahalingam R, Sunkar R（2017）. Isolation of Polysomal RNA for AnalyzingStress-Responsive Genes Regulated at the Translational Level in Plants. MethodsMol Biol. Humana press.1631:151-161. doi: 10.1007/978-1-4939-7136-7_9.
Li Y, Muthuramalingam M，Mahalingam M （2016），Plant responses totropospheric ozone.，Genetic manipulation in plants for mitigation of climatechange, Pawan Kumar Jaiwal, Rana Pratap Singh, Om Parkash, Springer press, pp1-14.
Li, YF. & Sunkar, R(2013). Global identification of small RNA targets by sequencing sliced ends ofmessenger RNAs. In Yang Y ed. Rice protocols: Methods in Molecular Biology,Humana press. 956:119-29. doi: 10.1007/978-1-62703-194-3_10
Muthuramalingam M, Li YF & Mahalingam R (2014),Genomics Based Analyses of Environmental Stresses In Crop. In Gaur R.K &Sharma P Eds. Approaches to Plant Stress and Their Management .383-394. DOI10.1007/978-81-322-1620-9_22, Springer India.
Sinai A, Li YF& Sunkar R (2012). Role of microRNAs in plant adaptation to environmentstress. In Sunkar R ed. MicroRNAs in Plant Development and Stress Responses(Signaling and Communication in Plants). Springer press. pp 219-232