生物增强活性炭工艺中优势菌群生物活性强化

郜玉楠¹, 李伟光^1,2,3, 王广智¹, 张多英¹, 刘水¹

1.哈尔滨工业大学市政环境工程学院;2.哈尔滨工业大学城市水资源开发利用(北方)国家工程研究中心;3.哈尔滨工业大学水资源与水环境国家重点实验室



摘要:

为提高生物增强活性炭工艺中优势菌的生物活性,使优势菌保持较高有机物降解能力,通过研究温度、pH值、培养时间、溶解氧对优势菌群生物活性的影响,确定优势菌的最佳生长条件.从松花江水中筛选可用于生物增强活性炭技术的优势菌5株,鉴定结果分别为Pseudomonas balearica,Pseudomonas putida,Acinetobacter calcoaceticus,Acinetobacter lwoffii,Brevibacterium mcbrellneri.结果表明,该5株优势菌在pH=6、温度为18℃、培养时间为36h、溶解氧为7mg/L条件下驯化后具有较高的脱氢酶活性,PCR-DGGE分析结果表明,在优化条件下得到的高活性优势菌群在活性炭上固定的数量要明显高于未在优化条件下生长的优势菌群.

关键词:  生物增强活性炭  生物活性强化  优势菌  培养条件

DOI：10.11918/j.issn.0367-6234.2010.10.019

分类号:TQ424.1

基金项目:国家自然科学基金资助项目(50678046)



Enhanced bio-activation of dominant of bacteria for enhancement of biological activated carbon process



1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.Nation Engineering Research Center of Urban Water Resources,Harbin Institute of Technology,Harbin 150090,China;3.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China)

Abstract:

To improve the biological activity of dominant bacteria on bio-enhanced activated carbon filter,five kinds of dominant bacteria,Pseudomonas balearica,Pseudomonas putida,Acinetobacter calcoaceticus,Acinetobacter lwoffii,and Brevibacterium mcbrellneri,which were isolated from Songhua River,were investigated for the enhanced biological activity.The effects of temperature,pH value,incubation time,DO value on the dehydrogenase activity variation of the dominant bacteria were studied in this paper.The results show that the dehydrogenase activity is high at 18 ℃,the pH value of 6,incubation time of 48 h and DO value of 7 mg/L,which is the optimal enhanced process for dominant bacteria.PCR-DGGE results show that the biomass of the dominant bacteria on activated carbon under the optimal enhanced process is more than that without the treatment of optimal enhanced process.

Key words:  bio-enhanced activated carbon process  enhanced bio-activation  dominant bacteria  culture condition