Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum
ZHANG Jun-zheng, Yang Qian, Yang Lie
Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China
Abstract:
Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.
Key words: sclerotinia sclerotiorum green fluorescent protein transformation hygromycin resistance gene protoplast
DOI：10.11916/j.issn.1005-9113.2009.03.009
Clc Number:S435.654
Fund:
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Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum
本站小编 哈尔滨工业大学/2019-10-23
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