姓　　名:易犁
办公电话:**-8039；
电子邮件:liyi@hubu.edu.cn, li_yi@hotmail.com
通讯地址:湖北省武汉市武昌区友谊大道368号
湖北大学生命科学学院
基本情况简介：博士，现任湖北大学教授
主要社会兼职：湖北省生物工程学会理事
主要研究领域：酶工程，分子进化，系统免疫学，酶的化学生物学，和高通量筛选技术

学习和工作简历：
?1996年9月-2000年7月，华中农业大学微生物系，获微生物学学士学位
?2000年9月-2003年7月，华中农业大学微生物系，获微生物学硕士学位
?2004年8月-2007年7月，美国内布拉斯加大学，获生物化学硕士学位
?2007年8月-2010年4月，美国密歇根大学安娜堡分校，获生物化学博士学位
?2010年11月-2012年5月，美国德州大学奥斯汀分校，博士后
?2012年6月-2014年1月，美国德州大学奥斯汀分校，副研究员（Research Associate)
?2014年2月-2014年8月，美国德州大学奥斯汀分校，研究员（Research Fellow)
?2014年9月-今，湖北大学生命科学学院教授

承担科研项目情况：
1.生物资源绿色转化湖北省协同创新中心人才引进课题资助（湖北省科技厅）：针对具有工业医用效应酶的分子改造及特性研究2014,9-2019,8，主持人。
2.2015年湖北省自然科学基金重点项目（湖北省科技厅，2015CFA088 ）：酵母内质网全蛋白酶图谱的绘制及提高人源溶栓蛋白酶表达的研究2015,1-2017,12，主持人。

代表性论文（*通讯作者）：
1.Yi, L.,# Li, Q.,# Hoi, K. H., Georgiou, G., and Iverson, B. L., “Mapping the Endogenous Protease CleaveOme in the Yeast Secretory Pathway and Recombinant Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing”, 2015, Submitted (#: co-1st author)
2.Zhou, Y., Mei, M., Yan, H., Ma, L., and Yi, L.* “The Application of Yeast Surface Display in Next-generation Non-antibody Protein Engineering”, 2015, Submitted
3.Zhang, L., Yu, C., Ma, L., and Yi, L.* “Effector proteins of Yersinia in Cell Infection”, 2015, Submitted
4.Yi, L.,* Li, Q., Taft, J. M., Gebhard, M. C., Iverson, B. L., and Georgiou, G. “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast”, Methods Mol. Biol. 2015, Invited chapter.
5.Li, Q., Yi, L., and Marek, P. “Antibodies and Their Multivalent Constructs for Cancer Therapy”, Protein & Peptide Lett. 2014 Jun 25.
6.Yi, L.,Gebhard, M. C., Li, Q., Taft, J. M., Georgiou, G., and Iverson, B. L. “Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatiorial libraries”, PNAS, 2013, 110(18):7229-34
7.Li, Q., Yi, L.,Marek, P., and Iverson, B. L. “Commercial proteases: present and future.”,FEBS Lett. 2013, 587(8):1155-63.
8.Ragsdale, S. W., Yi, L., et al. “Redox, Haem and CO in Enzymatic Catalysis and Regulation”, Biochem. Soc. Trans.,2012, 40(3):501-7.
9.Ragsdale, S. W.,and Yi, L. “Thiol/Disulfide Redox Switches in The Regulation of Heme Binding to Proteins”, Antioxid. Redox Signal.,2011, 14(6):1039-1047.
10.Yi, L., Morgan, J. T., and Ragsdale, S. W. “Identification Of a Thiol/Disulfide Redox Switch in The Human BK Channel That Controls Its Affinity For Heme And CO”, J. Biol. Chem., 2010, 285(26):20117-20127.
11.Gardner, J. D., Yi, L., Ragsdale, S. W., and Brunold, T. C. “Spectroscopic Insights Into Axial Ligation And Active-Site H-Bonding in Substrate-Bound Human Heme Oxygenase-2”, J. Biol. Inorg. Chem., 2010, 15(7):1117-1127.
12.Yi, L., Jenkins, P. M., Leichert, L. I., Jakob, U., Martens, J. R., and Ragsdale, S. W. “Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to The Cellular Redox State” J. Biol. Chem., 2009, 284, 20556-20561.
13.Bianchetti, C. M., Yi, L.,Ragsdale, S. W., and Philips, G. N., Jr."Comparison Of Apo- And Heme-Bound Crystal Structures Of a Truncated Human Heme Oxygenase-2", J. Biol. Chem.,2007, 282, 37624-37631.
14.Yi, L., and Ragsdale, S. W. "Evidence That The Heme Regulatory Motifs in Heme Oxygenase-2 Serve As a Thiol/Disulfide Redox Switch Regulating Heme Binding", J. Biol. Chem., 2007, 282, 20156-21067.
15.Yi, L., Wang, W., Li, M. Y., and Liu H. Z., “A Study Of The Geographical Differentiation Of RhodeusOcellatusBased On RAPD Analysis”,Acta. Hydrobiol. Sin., 2001, 25(3):301－304.

专利:
1.Method for Engineering Proteases and Protein Kinases. Brent L. Iverson, George Georgiou, Joseph M. Taft, and Li Yi, 2014, US PatentNO., 8,945,855; Canadian Patent Application NO., 2,877,346;European Patent Application NO., **.7

获奖：
1.Rackham Travel Award, University of Michigan at Ann Arbor, 2010
2.Anthony and Lillian Lu Award, University of Michigan at Ann Arbor, 2009
3.Rackham Travel Award, University of Michigan at Ann Arbor, 2009

国际会议特邀报告：
1.“YESS: A New Protease Engineering Platform”, Section of Advances in Biocatalysis, 246th National Annual Meeting of American Chemical Society, 2013, Indianapolis, IN, USA