姓　　名:李顺意
办公电话:**-8040
电子邮件:lisy@hubu.edu.cn
通讯地址:湖北省武汉市武昌区友谊大道368号湖北大学生命科学学院
基本情况简介：博士，现任湖北大学副教授、硕士研究生导师
主要社会兼职：湖北省生化与分子学会理事、湖北省生物物理学会理事
主要研究领域：生物化学与分子生物学、蛋白质化学


学习和工作简历：
?1991年9月-1994年7月，湖南师范大学生物学系，理学硕士
?1994年9月-1997年7月，中国科学院昆明动物研究所，理学博士
?2004年2月-2008年2月，美国佐治亚州立大学化学系，博士后
?1997年-今，湖北大学生命科学学院讲师、副教授


科研项目：
1.钙离子与蛇毒磷脂酶A2的结构和功能的关系研究，教育部留学人员回国人员基金，2011-2013年，主持
2.湖北省自然科学基金重点项目（2010CDB04507，2010），主持
3.湖北省教育厅自然科学基金（DC**，2010），主持

主要论文：
1.Li S, Jiang J., Zou J, Qiao J, Xue S, Wei L, Long R, Wang L, Castiblanco A, Maor N, Ngo J, Mao H, Liu L, and Yang J.J. PEGylation of Protein-based MRI Contrast Agents Improves Relaxivities and Biocompatibilities. Journal of Inorganic Biochemistry, 2012, 107(1), 111-118.
2.Zhang G, Li S, et al. Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10. Extremophiles, 2012, 16, 35-42.
3.Qiao J, Li S, Jiang J, Wei L, Liang R., Wang L., Mao H., Yang H., Grossniklaus H., Liu Z. and Yang J.J. HER-2 Targeted Molecular MR Imaging Using a de novo Designed Protein Contrast Agent. PLoS ONE, 6, e18103, 2011.
4.Wei, L; Li, S; Yang, J; Ye, Y.; Zou, J; Wang, L; Long, R; Zurkiya, O; Zhao, T; Johnson, J; Qiao, J; Zhou, W; Castiblanco, A; Maor, N; Chen, Y; Mao, H; Hu, X; Yang, JJ; Liu, ZR. Protein-based MRI Contrast Agents for Molecular Imaging of Prostate Cancer. Mol Imaging Biol, 2010, 13, 416-423.
5.Li, S; Yang, W; Maniccia, A; Barrow, D, Jr; Tjong, H; Zhou, H; Yang, J. Rational design of a Ca2+-binding protein with switchable conformation. FEBS （Federation of European Biochemical Societies) Journal, 275, 5048-5061, 2008.
6.Chen, N; Ye, Y; Zou, J; Li, S; Wang, S; Martin, A; Wohlhueter, R; Yang, J. Fluorescence complementation via EF-hand interactions. J Biotechnol. 2009, 142, 205-213.
7.Zhou, Y; Tzeng, W; Ye, Y; Huang, Y; Li, S; Chen, Y; Frey, T; Yang, J. A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication. Biochem J. 2009, 417, 477-483.
8.Maniccia, A; Yang, W; Johnson, J; Li, S; Tjong, H; Zhou, H; Shaket, L; Yang, J. Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteins. PMC Biophys. 2009, 2, 11.
9.Yang, J; Yang, J; Wei, L; Zurkiya, O; Yang, W; Li, S; Zou, J; Zhou, Y; Maniccia, A; Mao, H; Zhao, F; Malchow, R; Zhao, S; Johnson, J; Hu, X; Krogstad, E; Liu, Z. Rational Design Protein Based MRI Contrast Agents with High Relaxivity Journal of the American Chemical Society. 2008, 130, 9260-9267.
10.Huang, Y; Zhou, Y; Yang, W; Butters, R; Lee, H; Li, S; Castiblanco, A; Brown, E; Yang, J. Identification and Dissection of Ca2+-Binding Sites in the Extracellular Domain of Ca2+-sensing Receptor. Journal of Biological Chemistry. 2007, 282, 19000-19010.
11.Maniccia, A; Yang, W; Li, S; Johnson, J; Yang, J. Using protein design to dissect the effect of charged residues on metal binding and protein stability. Biochemistry. 2006, 45(18): 5848-5856.
12.Yang, W; Wilkins, A; Li, S; Ye, Y; Yang, J. The effects of Ca2+ binding on the dynamic properties of a designed Ca2+–binding protein. Biochemistry. 2005, 44(23): 8267-8273.
13.Yang, W; Wilkins, A; Ye, Y; Liu, Z; Li, S; Urbauer, J; Hellinga, H; Kearney, A; van der Merwe, P; Yang, J. Design of a calcium-binding protein with desired structure in a cell adhesion molecule. J AM CHEM SOC. 2005, 127, 2085-2093