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杨小淦
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硕士研究生导师,博士研究生导师
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主持在研科研项目3项,其中包括国家自然科学基金面上项目1项,广西自然科学基金面上项目1项,广西自然科学基金重点项目1项(合作),发表文章80多篇,获广西科技进步奖一等奖1项。
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本科课程:生物统计附试验设计硕士研究生课程:基因工程技术及应用基因组与生物信息学现代农业创新与乡村振兴战略博士研究课程:动物的生长与发育
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一、家畜配子及早期胚胎发育规律及机理;动物精原干细胞自我更新和增殖分子机制。二、动物生产新技术研发。
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序号 项目名称 1 β-巯基乙醇诱导自噬调控水牛体外胚胎细胞存活和死亡的机制研究 2 Nanos2维持水牛精原干细胞干性特征机制的研究 3 罗汉果来源天然甜味剂治疗PCOS的效果和机制研究 4 Nanos2维持水牛精原干细胞干性机制的研究 5 广西畜牧产业科技先锋队 6 水牛精原干细胞体外诱导精子的研究 7 基于激光光镊拉曼光谱的水牛XY分离精子损伤评价体系的研究 8 广西三黄鸡诱导多能干细胞建系的合作研究 9 水牛卵母细胞玻璃化冷冻的研究与应用 10 水牛XY分离精子产业化推广应用 11 高产良种奶水牛的繁育技术研究 12 microRNA调控小鼠早期胚胎发育全能或多能性机制的初步研究 13 猪胚胎玻璃化冷冻的初步研究 14 基于激光光镊拉曼光谱的水牛xy分离精子损伤评价体系的研究 15 不同季节对猪卵母细胞体外成熟的比较 16 利用抑制消减杂交筛选猪MII期卵母细胞差异转录本的研究
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序号 发表刊物 论文名称 1 PAKISTAN JOURNAL OF ZOOLOGY Overexpression of GDNF can Effectively Maintain the Pluripotency of Buffalo Spermatogonia In Vitro 2 ENVIRONMENTAL POLLUTION Constant light exposure causes oocyte meiotic defects and quality deterioration in mice 3 基因组学与应用生物学 鸡输卵管上皮细胞体外分离培养的研究 4 JOURNAL OF VETERINARY SCIENCE Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro 5 AGING-US Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation 6 REPRODUCTION IN DOMESTIC ANIMALS The effects of growth factors on proliferation of spermatogonial stem cells from Guangxi Bama mini‐pig 7 THERIOGENOLOGY Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development 8 development PHF7 is a novel histone H2A E3 ligase prior to histone-toprotamine exchange during spermiogenesis 9 PAKISTAN JOURNAL OF ZOOLOGY Evaluation of the Effect of Microoperation on Porcine Parthenogenetic Embryo Competence by Raman Spectroscopy 10 REPRODUCTION FERTILITY AND DEVELOPMENT Maturation of buffalo oocytes in vitro with acetyl-L\u0002carnitine improves cryotolerance due to changes in mitochondrial function and the membrane lipid profile 11 西南农业学报 水牛GV期和MⅡ卵母细胞中脂肪含量及分布模式研究 12 AGING-US Melatonin?prevents?deterioration?in?quality?by?preserving?epigenetic?modifications?of?porcine?oocytes?after?prolonged?culture 13 THERIOGENOLOGY Treatment with acetyl-L-carnitine during in vitro maturation of buffalo oocytes improves oocyte quality and subsequent embryonic development 14 JOURNAL OF VETERINARY SCIENCE In vitro differentiation of spermatogonial stem cells using testicular cells from Guangxi Bama mini-pig 15 ANIMAL REPRODUCTION SCIENCE Nanos2 is a molecular marker of inchoate buffalo spermatogonia 16 畜牧与兽医 水牛性控冷冻精液授精效果的研究 17 POULTRY SCIENCE Derivation and characterization of primordial germ cells from Guangxi yellow-feather chickens 18 CRYOLETTERS COMPARISON OF MITOCHONDRIAL FUNCTION IN BOAR AND BULL SPERMATOZOA THROUGHOUT CRYOPRESERVATION BASED ON JC-1 STAINING 19 PAKISTAN JOURNAL OF ZOOLOGY Characterization of hGFAP-DsRed Transgenic Guangxi Bama Mini-Pigs and Their Offspring 20 ANIMAL REPRODUCTION SCIENCE Effect of the meiotic inhibitor cilostamide on resumption ofmeiosis and cytoskeletal distribution in buffalo oocytes 21 romanian biotechnological letters Increasing the number of transferred embryos results in delivery of viable transgenically cloned Guangxi Bama mini-pigs 22 3 Biotech Successful cloning of an adult breeding boar from the novel Chinese Guike No. 1 swine specialized strain 23 基因组学与应用生物学 桂科Ⅰ号成年公猪体细胞核移植胚胎的体外生产 24 GENETICS AND MOLECULAR RESEARCH Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro 25 molecular immunology Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5 26 PLOS ONE RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming 27 ANIMAL REPRODUCTION SCIENCE Flow cytometric and near-infrared Raman spectroscopic investigation of quality in stained, sorted, and frozen-thawed buffalo sperm 28 基因组学与应用生物学 利用胞浆内单精子注射技术研究水牛与猪的种间受精:阶段性报告 29 cryobiology Stability of the cytoskeleton of matured buffalo oocytes pretreated with cytochalasin B prior to vitrification 30 in vitro cellular & developmental biology - animal In vitro production of cloned and transgenically cloned embryos from Guangxi Huanjiang Xiang pig 31 基因组学与应用生物学 25-羟基胆甾醇对鸡原始生殖细胞增殖的影响 32 基因组学与应用生物学 Scriptaid 在猪体细胞核移植应用的研究进展 33 GENETICS AND MOLECULAR RESEARCH Production of hGFAP-DsRed transgenic Guangxi Bama mini-pigs via somatic cell nuclear transfer 34 THERIOGENOLOGY A field study on artificial insemination of swamp and crossbred buffaloes with sexed semen from river buffaloes 35 基因组学与应用生物学 水牛精原干细胞研究进展 36 基因组学与应用生物学 不同日龄巴马小型猪睾丸内精原干细胞的鉴定 37 基因组学与应用生物学 巴马小型猪转基因克隆胚胎融合/激活条件的优化 38 南方农业学报 基于AMEL基因位点差异的水牛性别PCR鉴别方法 39 南方农业学报 丝裂霉素C对制备干细胞饲养层效果的影响 40 基因组学与应用生物学 哺乳动物精原干细胞诱导分化研究进展 41 MOLECULAR REPRODUCTION AND DEVELOPMENT ZPAC is required for normal spermatogenesis in mice 42 基因组学与应用生物学 影响哺乳动物卵母细胞玻璃化冷冻保存效果的因素 43 基因组学与应用生物学 叶酸对猪卵母细胞体外成熟后微管和微丝分布的影响 44 中国兽医学报 叶酸对猪卵母细胞体外成熟的影响 45 南方农业学报 水牛卵母细胞玻璃化冷冻后孤雌激活方法的优化 46 基因组学与应用生物学 蔗糖和海藻糖对水牛卵母细胞玻璃化冷冻保护效果的比较 47 in vitro cellular & developmental biology - animal In vitro development of porcine transgenic nuclear-transferred embryos derived from newborn Guangxi Bama mini-pig kidney fibroblasts 48 in vitro cellular & developmental biology - animal Establishment and biological characteristics comparisonof Chinese swamp buffalo (Bubalus bubalis ) fibroblast cell lines 49 基因组学与应用生物学 玻璃化冷冻对哺乳动物卵母细胞骨架系统的影响 50 中国兽医学报 不同离心液对猪孤雌激活胚胎的冷冻效果 51 buffalo blletin sperm sexing in buffalo using flow cytometry 52 第七次全国动物生物技术学术研讨会 shRNA扰低水牛胎儿皮肤成纤维细胞H1c基因表达的研究 53 南方农业学报 不同月份猪卵母细胞体外成熟效果比较 54 Zygote Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2'-deoxycytidine 55 西南农业学报 不同生理状态及激素量对小鼠超数排卵及后续胚胎发育的影响 56 中国畜牧杂志 不同发育天数及囊胚类型对猪孤雌激活胚胎冷冻效果的研究 57 buffalo blletin A two step culture system sustains individual buffalo embryo development 58 JOURNAL OF ANIMAL AND VETERINARY ADVANCES Addition of Glutathione to Semen Extender During Sperm Sorting can Improve in vitro Embryonic Development and in vivo Fertility in Buffalo 59 REPRODUCTION IN DOMESTIC ANIMALS Flow cytometric and NIR (near-infrared) Raman spectroscopic investigation of sperm quality in the strained, sorted and frozen-thawed buffalo spermatozoa 60 REPRODUCTION IN DOMESTIC ANIMALS Sorted sperm decreased the sperm quality and subsequent embryonic development after in vitro fertilization of oocytes in buffalo 61 REPRODUCTION IN DOMESTIC ANIMALS Investigation of sperm quality in the stained,sorted and frozen-thawed buffalo spermatozoa using flow cytometric and NIR(near-infrared) Raman Spectroscope 62 REPRODUCTION IN DOMESTIC ANIMALS Melatonin enhances bufflao oocyte maturation and embryo development in vitro 63 animal science papers and reports cDNA cloning of porcine CDH1 and its expression profile in porcine early parthenotes 64 中国兽医学报 广西巴马小型猪Oct4基因克隆及其在成纤维细胞中的表达 65 南方农业学报 瘦素对水牛卵母细胞成熟和体外受精胚胎发育的影响 66 中国兽医学报 人神经母细胞瘤细胞异种克隆胞质受体的选择 67 Production in Domestic Animals Freezing procedure can result in more serious apoptosis of boar spermatozoa than bull spermatozoa 68 Production in Domestic Animals cDNA Cloning of Porcine E-cadherin and its expression profile in porcine early parthenotes 69 Production in Domestic Animals Effects of Melatonin against Oxidative stress on Flow Cytometry Sorted Buffalo Sperm and Subsequent in Vitro Embryo Development 70 Production in Domestic Animals Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2’-deoxycytidine 71 Production in Domestic Animals Tacrine can significantly improve motility of frozen–thawed boar spermatozoa 72 Production in Domestic Animals Sex-preselected piglets derived from surgical artificial insemination with sexed sperm 73 畜牧兽医学报 猪卵母细胞化学辅助手工去核及手工核移植胚胎发育能力因素的研究 74 中国农业科学 猪早期孤雌胚组蛋白乙酰化与组蛋白去乙酰化酶基因mRNA表达水平相关性研究 75 中国兽医学报 3种来源猪胚胎激活后6h内基因组DNA甲基化变化模式 76 中国兽医学报 电融合/激活对于巴马小型猪体细胞克隆胚胎生产的影响 77 中国兽医学报 食蟹猴-猪异种体细胞核移植的初步研究 78 中国畜牧杂志 水牛性控精液微生物分析 79 畜牧兽医学报 猪手工体细胞核移植电融合/激活参数的优化 80 CELLULAR REPROGRAMMING Cloned Guangxi Bama minipig (Sus scrofa) and its offspring have normal reproductive performance 81 中国兽医学报 广西巴马小型猪供核细胞的传代培养和冷冻保存及肌肉成纤维细胞HDAC1基因的mRNA的表达 82 Production in Domestic Animals In Vitro Culture and Differentiation of Buffalo (Bubalus bubalis) Spermatogonia
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序号 专利授权日 专利名称 1 2015-11-04 一种猪精原干细胞的分离方法 2 2015-10-21 一种巴马小型猪精原干细胞诱导精子细胞的方法 3 2015-10-21 一种猪精原干细胞冻存液及其使用方法
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序号 奖励名称 获奖等级 成果名称 1 广西科技进步奖 一等奖 水牛XY精子分离性别控制技术研究及应用
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欢迎热爱动物科学研究(学术)和热爱动物生产(专硕)的同学加入我的科研团队。"}},
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广西大学动物科学技术学院导师教师师资介绍简介-杨小淦
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