a) The initiator box is downstream of both the AUG start codon and the TATA box
b) The TATA box is downstream of the upstream elements and upstream of the initiator box
c) The upstream elements are upstream of the promoter but downstream of the transcriptional start site
d) The TATA box is upstream of the transcriptional start site but downstream of the initiator box
e) The initiator box is upstream of the first exon and downstream of the translational start site
7. Addition of polyA to the 3' end of mRNA requires（ ）
a). polyA polymerase (PAP)
b). RNA cleavage
c). CPSF
d). a and c
e). all of the above
8. The transcription factor, SL1, which promotes transcription by RNA polymerase I, also（ ）
a). binds to both Core and Upstream elements;
b). contains TBP;
c) binds before the RNAP;
d). both a and c;
e). all of the above.
9．以下对于一般转录因子的陈述哪一个是错误的? （ ）
a)TFⅡD结合TATA框
b)TFⅡD是由TBP和TAFⅡ组成的多蛋白复合体
c)TBP是RNA聚合酶Ⅰ、Ⅱ和Ⅲ转录中的共同因子
d) TFⅡB使TFⅡD-DNA复合体稳定
e)RNA聚合酶结合后，TFⅡE、TFⅡH和TFⅡJ与转录复合体结合
f)TFⅡH使CTD磷酸化
10．以下对于转录因子的陈述哪两个是正确的? （ ）
a)螺旋一转角一螺旋结构域是转录激活结构域
b)转录因子的二聚化发生在碱性结构域
c)亮氨酸拉链与DNA结合
d)当来自几个转录因子的DNA结合区和激活区融合在一起时，得到功能性转录因子是可能的
e)转录因子的同一结构域即可作为阻抑蛋白也可作为激活结构域
三、填空题：
1. A transcription terminator consists of a ____________ structure made from ____________-repeats followed by a string of U's (in the mRNA).
2. Promoter can require binding of to form a transcription complex.
3. During polyadenylation, CPSF binds to a _______ sequence and cooperates to promote __ ____ (factor) binding to the downstream GU/U rich region. Cleavage factors ______ then cleave the mRNA, and polyA polymerase adds an average of ___________ adenines to the 3’ end.
4. .Type 1, Type 2 promoters directed pol III are located downstream of .
5. CTD phosphorylation correlates with ___________.
6. Which general transcription factor is the assembly factor in class I promoter? In other words,which binds first and helps the other bind?
7.真核生物的mRNA加工过程中，5’端加上 ，在3’端加上 ，后者由 催化。如果被转录基因是不连续的，那么， 一定要被切除，并通过 过程将 连接在一起。这个过程涉及许多RNA分子，如Ul和U2等，它们被统称为 。它们分别与一组蛋白质结合形成 ，并进一步地组成40S或60S的结构，叫 。
四、问答题：
1. What are the roles of Sigma and Rho?
2. Describe some cis-acting elements by eukaryotic RNA POL I,II and III, as you know.
3. Describe transcription in eukaryotes. Include in your discussion promoters, start signal, stop signal, primary transcript, introns, exons, cap and poly-A tail.
4. List in order the proteins that assemble in vitro to form a class II preinitiation complex.
5. What are required roles for basal factors?
6. 你所认识的真核生物RNA 聚合酶Ⅰ、Ⅱ和Ⅲ识别模板上的顺式作用因子有哪些？
7. 真核生物RNA 聚合酶Ⅰ、Ⅱ和Ⅲ的起始因子中有哪些含有TBP蛋白。
8. 真核生物转录因子至少含有哪两个功能？
习题九
一、名词解释：
hnRNA，RNA splicing，GT-AG rule，spliceosome，cis-splicing，trans-splicing， ribozyme，alternative splicing，RNA editing，guide RNA,
