a)它们通过保守的亮氨酸残基与 DNA结合
b)它们与 HLH蛋白相似之处是：它们都具有相邻的 DNA结合结构域和二聚化的 结构域
C)Jun蛋白可以形成同源二聚体而 Fos蛋白不可以
d) Fos／Jun复合物与 Jun／Jun复合物结合的 DNA序列不同
e) Fos／Jun与 DNA的结合比 Jun／Jun牢固
三、填空题：
1. Steroid homone receptors, when bound by an appropriate hormone, bind to
2. An mRNA with many AUUUA sequences in its 3’ UTR will be _________.
3. Enhancers in yeast are called
4. A DNA element that can act at a distance to decrease transcription from a eukaryotic gene calls .
四、问答题：
1. Describe expression regulation of GAL gene by GAL4/80protein.
2. What chemical modification of DNA is known to be important in eukaryotic gene regulation?
3. How does histone acetylation affect the gene expression in eukaryotic cells?
4. How do IRE and IRE binding protein control synthesis of transferrin-receptor and ferritin?
5. Identify the following consensus sequences (what process or system they're used in and their basic function...you don't need to list names of factors that bind unless you want to)
6. List and brief describe three different classes of DNA-binding domains found in eukaryotic transcription. (200-300 words)
7.下列DNA分子利用Hela细胞抽提液在理想条件下, 进行体外转录和翻译, 并用相应的探针进行Northern blot杂交和Western blot杂交,预计将产生怎样的结果? (1) 一个长为7500bp的鸡卵清蛋白基因。(2) 一个长为1859bp的鸡卵清蛋白的cDNA分子。(3) 长为1859bp的鸡卵清蛋白的cDNA分子上游连接上能为RNA聚合酶Ⅱ 识别的启动子。(4) 长为1859bp的鸡卵清蛋白的cDNA分子上游连接上能为RNA聚合酶Ⅲ 识别的启动子(原tRNA基因的启动子)。(5) (3)题中DNA分子启动子上游又接上一个反方向的增强子(enhancer) 。
8.DNA序列的甲基化、组蛋白的乙酰基化基因转录有什么影响？
9.类固醇激素受体蛋白在序列和空间结构上可分为哪两个部分（domain）,功能是什么？
10.真核生物一些mRMA 3’末端非翻译序列含有多拷贝的AUUUA，该序列功能是什么？
