顾颖，博士 GU Ying, Ph.D.
副教授，硕士生导师 Associate Professor
电话：+86-
传真：+86-
E-mail：guying@xmu.edu.cn

简历 (Education and Work experience)
1996年，厦门大学生物学专业，学士学位；
2006年，厦门大学生化与分子生物学专业/细胞生物学专业，博士学位；
2006年，厦门大学生命科学学院助理教授、硕士生导师；
2013年，厦门大学生命科学学院副教授；
2018-2019年，美国国立卫生研究院过敏与传染病研究所疫苗研究中心（NIH-NIAID-VRC），访问****

B.S. Biochemistry, Xiamen University, 2000

Ph.D. Biochemistry & Molecular Biology/Cell Biology, Xiamen iversity, 2006

Assistant Professor, School of Life Sciences, Xiamen University, 2006

Associate Professor, School of Life Sciences, Xiamen University, 2013

Visiting Scholar, Vaccine Research Center (VRC), National Institute of Allergy and Infectious Diseases (NIAID), National Health Institute (NIH), 2018-2019
研究领域 (Research Area)

抗病毒的单抗筛选：利用细胞模型和重组抗原等进行抗病毒的单抗筛选，如HIV中和单抗、识别免疫优势表位单抗等的筛选。
基于表位的抗原研究：利用基因工程重组技术表达获得含有功能表位的抗原或颗粒化抗原。
功能性表位研究：应用生物传感器、分析超离、微量热等方法研究抗原-抗体的相互作用，解析免疫复合物的空间结构，鉴定功能性表位的特征。
Generation of monoclonal antibodies against different viral targets : based on functional screening by virus-infection cell model, recombinant antigen reactivity and et. al, focusing on HIV neutralizing monoclonal antibodes, esp. those with broad neutralizing activity
Identification and characterization of key epitopes on viral antigens: Design and preparation of recombinant antigens harbouring functional epitopes
Functional characterization of epitopes: epitope mapping, structural analysis and function interpretation by various methods including SPR-based assay, analytical ultracentrifugation, microcalorimetry, and structural determination of antibody-antigen complexes


代表性论文(Selected Publications)(* Corresponding author)：
1. Shen CH, DeKosky BJ, Guo Y, Xu K, Gu Y, Kilam D, Ko SH, Kong R, Liu K, Louder MK, Ou L, Zhang B, Chao CW, Corcoran MM, Feng E, Huang J, Normandin E, O'Dell S, Ransier A, Rawi R, et al. VRC34-Antibody Lineage Development Reveals How a Required Rare Mutation Shapes the Maturation of a Broad HIV-Neutralizing Lineage. Cell Host Microbe. 2020 Apr 8;27(4):531-543.e6. doi: 10.1016/j.chom.2020.01.027. Epub 2020 Mar 3.

2. Zheng Q, Jiang J, He M, Zheng Z, Yu H, Li T, Xue W, Tang Z, Ying D, Li Z, Song S, Liu X, Wang K, Zhang Z, Wang D, Wang Y, Yan X, Zhao Q, Zhang J, Gu Y, Li S, Xia N. Viral neutralization by antibody-imposed physical disruption. Proc Natl Acad Sci U S A. 2019 Dec 9;116(52):26933–40. doi: 10.1073/pnas.. Epub ahead of print.

3. Liu X, Chen J, Wang Z, Wang D, He M, Qian C, Song S, Chi X, Kong Z, Zheng Q, Wang Y, Yu H, Zhao Q, Zhang J, Li S, Gu Y, Xia N. Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection. Emerg Microbes Infect. 2019;8(1):1721-1733.

4. Zhang Z, Wei X, Lin Y, Huang F, Shao J, Qi J, Deng T, Li Z, Gao S, Li S, Yu H, Zhao Q, Li S, Gu Y, Xia N. HIV-1 Membrane-Proximal External Region Fused to Diphtheria Toxin Domain-A Elicits 4E10-Like Antibodies in Mice. Immunol Lett. 2019 Sep;213:30-38. doi: 10.1016/j.imlet.2019.07.004. Epub 2019 Jul 26.

5.Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, Cheng C, Dingens AS, Gorman J, Sastry M, Shen CH, Zhang B, Zhou T, Chuang GY, Chao CW, Gu Y, Jafari AJ, Louder MK, O'Dell S, Rowshan AP, Viox EG, Wang Y, Choi CW, Corcoran MM, Corrigan AR, Dandey VP, Eng ET,et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019 Jul 25;178(3):567-584.e19.

6.Li T, Zhang Z, Zhang Z, Qiao J, Rong R, Zhang Y, Yao Q, Li Z, Shen H, Huang F, Xue W, Gao S, Li S, Zheng Q, Yu H, Zhang J, Gu Y*, Li S*, Xia N. Characterization of native-like HIV-1 gp140 glycoprotein expressed in insect cells. Vaccine. 2019 Feb 5. pii: S0264-410X(19)30124-0. doi: 10.1016.

7. Zhang Z, Zhang F, Bai S, Qiao J, Shen H, Huang F, Gao S, Li S, Gu Y*, Xia N. Characterization and epitope mapping of a panel of monoclonal antibody against HIV-1 matrix protein. Biotechnol Appl Biochem. 2018 Apr 17. doi: 10.1002/bab.1662.

8. Zhang Z, Wang L, Bai S, Qiao J, Shen H, Huang F, Gao S, Li S, Li S, Gu Y*, Xia N. Expression, Purification and Characterization of HIV-1 Capsid Precursor Protein p41. Protein J. 2018 Mar 5. doi: 10.1007/s10930-018-9763-0.

9. Li, Z., S. Song, M. He, D. Wang, J. Shi, X. Liu, Y. Li, X. Chi, S. Wei, Y. Yang, Z. Wang, J. Li, H. Qian, H. Yu, Q. Zheng, X. Yan, Q. Zhao, J. Zhang, Y. Gu, S. Li and N. Xia (2018). "Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity." Nat Commun 9(1): 5360.

10. Li Z, Wang D, Gu Y, Song S, He M, Shi J, Liu X, Wei S, Li J, Yu H, Zheng Q, Yan X, Baker T S, Zhang J, McLellan JS*, Li S*, Xia N*. (2017) Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus. MBio 8, 5

11. Gu, Ying#; Wei, Minxi# ; Wang, Daning; Li, Zhihai; Xie, Minghui; Pan, Huirong; Wu, Ting; Zhang, Jun; Li, Shaowei*; Xia, Ningshao*, Characterization of an Escherichia coil-derived human papillomavirus type 16 and 18 bivalent vaccine, VACCINE, 2017.8.16, 35(35): 4637~4645.

12. Zhang Z, Li S, Gu Y*, Xia N. Antiviral Therapy by HIV-1 Broadly Neutralizing and Inhibitory Antibodies. Int J Mol Sci. 2016 Nov 18;17(11).

13. Zhang Z, Zhang F, Yang C, Qi J, Gao S, Li S, Gu Y*, Xia N. HIV-1 Capsid as a Target for Antiviral Therapy. J AIDS Clin Res 7:536. 2016 Jan. doi:10.4172/2155-6113.**

14.Yuan R, Qi J, Zhang Z, Li S, Gu Y*, Xia N. Anti-CD4: An Alternative Way to Inhibit HIV Infection. J HIV & Retro Virus. 2016 Jan.

15. Hou W, Fang C, Liu J, Yu H, Gu Y* et al. Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody. Antiviral Res. 2015 Aug; 122:101-11.

16.Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 May;25(5):604-20.

17. Wang D, Li Z, Xiao J, Wang J, Zhang L, Liu Y, Fan F, Xin L, Wei M, Kong Z, Yu H, Gu Y, Zhang J, Li S, Xia N. Identification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody. PLoS One. 2015 Apr 23;10(4):e**.

荣誉、奖励及参加学术团体的情况(Honors and award)
主持3项国家自然科学基金项目、1项国家科技重大专项子课题，1项福建省科技重大项目，并作为主要参与人参加多项国家级、省部级重大重点科研项目；获得中国高校科技进步一等奖（2001），国家技术发明二等奖（2010），中国专利金奖（2012），厦门市科学技术进步一等奖（2010），厦门大学第四届青年教师教学技能比赛一等奖（2008），厦门大学第三届青年教师教学技能比赛二等奖（2007）。申请发明专利11项，国际发明专利8项，获得授权4项；为主参与研制戊肝疫苗、宫颈癌疫苗、艾滋诊断试剂等新药。