研究生导师介绍
姓名：廖飞性 别:男?院 系：检验医学院
行政职务：专业技术职称：教授导师类别：博士生导师
从事专业1：生物物理学从事专业2：从事专业3：
最后学历：最后学位：硕士任硕导年月：200206
任博导年月：200603是否院士：否是否国务院学科评议组成员：否
毕业院校：兰州大学毕业专业：071011 生物物理学毕业时间：1993.6.28
办公电话：**E-mail：liaofeish@cqmu.edu.cn是否停招：否
◇ 个人简历：
2005年晋升教授，博士生导师；入选2009年教育部新世纪优秀人才支持计划；重庆市第二届学术与技术带头人后备人选；获“重庆市高校优秀中青年骨干教师”称号。
◇ 本人从事的主要研究方向：
分析探针与蛋白质生物技术
◇ ：在国内外核心期刊上发表学术论文情况
论文题目刊物名称刊物国家收录情况卷期排名
Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detectionMichrochim Acta 2012国外 SCI176(1):243-249通讯作者
Uricases as therapeutic agents to treat refractory gout: current states and future directionsDrug Dev Res, 2012国外 SCI73(2):66-72通讯作者
Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition typeJ Enzyme Inhibit Med Chem 2013国外 SCI 28(1):205-213通讯作者
Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acid residues and PEG derivativesBiodrugs, 2012国外 SCI26(4):209-215通讯作者
Kinetic analysis of gamma-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of gamma-glutamyl p-nitroanilineJ Zhejiang Univ Sci B, 2011国内 SCI12(3):180-188通讯作者
Uricase based method for determination of uric acid in serumMicrochim Acta 2009国外 SCI164(1):1-6通讯作者
An integration strategy to estimate the initial rates of enzyme reactions with much expanded linear ranges using uricases as modelsAnal Chim Acta 2009国外 SCI631(1):22-28通讯作者
The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite greenAnal Chim Acta 2009国外 SCI636(1):105-110通讯作者
Soluble expression in E. coli of active human cyclic nucelotide phosphodiesterase isoform 4B2 in fusion with maltose-binding-proteinBiosci Biotechnol Biochem, 2009国外 SCI73(4):968-970通讯作者
The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activationClin Biochem; 2009国外 SCI42(6):926-928通讯作者
Homogeneous noncompetitive assay of protein via F鰎ster-resonance-energy-transfer with tryptophan residue(s) as intrinsic donor(s) and fluorescent ligand as acceptorBiosens Bioelectron. 2009国外 SCI25(1):112-117通讯作者
Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strengthBiosci Biotechnol Biochem, 2009国外 SCI73(9):2141-2144通讯作者
Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosusBiosci Biotechnol Biochem. 2010国外 SCI74(6): 1298-1301.通讯作者
Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophan residues in a protein via Foster-resonance-energy-transferSpectrochim Acta A Mol Biomol Spectrosc 2010国外 SCI77(4):869-876.通讯作者
Kinetic analysis of lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategyAnal Sci 2010国外 SCI26(11):1193-1198通讯作者
A new practical system for evaluating pharmacological properties of uricase as a potential drug for hyperuricemiaArch Pharm Res, 2010国外 SCI33(11):1761-1769通讯作者
An Improved Malachite Green Assay of Phosphate: Mechanism and ApplicationAnal Biochem 2011国外 SCI409(1):144-149通讯作者
An integration strategy to measure enzyme activities for detecting irreversible inhibitors with dimethoate on butyrylcholinesterase as modelIntern J Environ Anal Chem, 2011国外 SCI91(5):431-439通讯作者
Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental modelBMC Biotechnol 2011国外 SCI11:44 (doi:10.1186/1472-6750-11-44)通讯作者
Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as modelChem Cent J, 2013国外 SCI7(1):69 (http://dx.doi.org/10.1186/1752-通讯作者
Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivativesChem Cent J 2012国外 SCI6(1):142 (http://dx.doi.org/10.1186/1752通讯作者
Chromogenic substrate from 4-nitro-1-naphthol for hydrolytic enzyme of neutral or slightly acidic optimum pH: 4-nitro-1-naphthyl-β-D-galactopyranoside as an exampleBioorg Med Chem Lett, 2013国外 SCI23(3):646-649通讯作者
Comparison of F謗ster-resonance-energy-transfer acceptors for tryptophan and tyrosine residues in native proteins as donorsJ Fluorescence, 2013国外 SCI23(1):147-157通讯
Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groupsInt J NanoMed, 2013国外 SCI8:791-807通讯作者
Spectrophotometric-dual-enzyme-simultaneous-assay in one reaction solution (SDESA): chemometrics and experimental modelsAnal Chem， 2013国外 SCI85 (4):2143-2154通讯作者
Fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione s-transferase.J Fluoresc, 2015国外 SCI25(1)：1-8通讯作者
Approximated maximum adsorption of His-tagged enzyme/mutants on Ni2+-NTA for comparison of specific activitiesInt J Biol Macromol, 2015国外 SCI74: 211-217.通讯作者
Comparison of candidate pairs of hydrolytic enzymes for spectrophotometric-dual-enzyme-simultaneous-assay Anal Sci 2015国外 SCI31(5): in press通讯作者
Crystal structure of Bacillus fastidious uricase reveals an unexpected folding of the C-terminus residues crucial for thermostability under physiological conditionsAppl Micriobiol Biotechnol 2015国外 SCIin press (DOI: 10.1007/s00253-015-6520-6通讯作者
Facile characterization of the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidinAppl Spectros, 2013国外 SCI67(6):688-691通讯作者
Two glycosidases as label enzymes for concurrent enzyme-linked-immunosorbent-assay of two components via spectrophotometric-dual-enzymesimultaneous-assay in one solutionAnal Methods， 2013国外 SCI 5(10): 5969-5976通讯作者
Resonant-Mie-scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymesAnal Chim Acta, 2013国外 SCI804:215-220通讯作者
Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor Anal Chim Acta， 2014国外 SCI829(1):60-67通讯作者
Design, synthesis and evaluation of novel quinazoline-2,4-dione derivatives as chitin synthase inhibitors and antifungal agentsBioorgan Med Chem, 2014国外 SCI22(13): 3405-3413通讯作者
Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nmAnal Chem, 2014国外 SCI86(12):5667-5672通讯作者
Microplate-based method to screen inhibitors of isozymes of cyclic nucleotide phosphodiesterase fused to SUMOJ Enzyme Inhibit Med Chem, 2014国外 SCI29(6):836-839通讯作者
Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)Biotechnol Appl Biochem, 2014国外 SCI61(6):683-690通讯作者
Optimization of pH values to formulate the bi-reagent kit for serum uric acid assayBiotechnol Appl Biochem, 2015国外 SCI62(1):137-144.通讯作者
Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wellsRSC Advance, 2014国外 SCI4(56): 29925-29932通讯作者
◇ ：出版专著教材情况(注：在书名后注明教材或专著)
名称类别出版单位日期排名
生物信息学， ISBN 978-7-117-12938-1教材人民卫生出版社2010-08-30第二副主编
Kinetic Analyses of Enzyme Reaction Curves with New Integrated Rate Equations and Applications，Chemometrics in Practical Applications (2012). pp.157-184. Kurt Varmuza (Ed.), ISBN: 978-953-51-0438-4 专著InTech 2012-05-05章节通讯作者
◇ ： 成果获奖情况
成果名称颁奖部门等级完成日期证书号排名
通过预测本底进行酶法分析定量测定体液尿酸含量的方法国家发明专利ZL **.4第一
一种重组表达苛求芽孢杆菌胞内尿酸酶及其聚乙二醇修饰与应用国家发明专利ZL**1.1第一
联用积分法和初速度法测定酶活性的方法国家发明专利ZL **1.4第一
以蛋白中色氨酸残基为Forster共振能量转移供体的均相亲和分析方法国家发明专利ZL **0.8第一
作用于血液中低分子物质的大分子药物的体外给药装置及其应用国家发明专利ZL **0.X第一
联用酶反应动力学过程分析法和终点平衡法测定酶底物量的方法国家发明专利ZL **6.5第一
用两端带羧基的直链亲水聚合物包被纳米磁芯制备微纳米磁性材料的方法及其应用国家发明专利ZL **9.5第一
用多波长吸收单通道同步测量多种酶活性的方法国家发明专利ZL **6.8第一
联用磁分离与色谱分析测定混合物中配体亲和力的方法国家发明专利ZL **8.2第一
用偶联酶的最大瞬时速度测定靶酶活性的方法国家发明专利ZL **0.X第一
◇ ：主持重大科研项目情况
项目名称任务来源完成形式完成日期鉴定验收单位主要结论排名
酶反应动力学过程分析的应用”，编号**结题报告国家自然科学基金委结题第一
混合物中靶蛋白高亲合力配体集群筛选方法的建立和应用”，编号 **结题报告国家自然科学基金委结题第一
苛求芽孢杆菌胞内尿酸酶的克隆表达、分子改造和应用”，编号**结题报告国家自然科学基金委结题第一
以蛋白质芳香族氨基酸残基为共振能量转移供体的均相亲和分析方法国家自然科学基金项目结题报告国家自然科学基金委结题第一
新型免疫检测技术的研究973、863项目结题报告科技部结题第一
◇ ：目前承担的主要项目
项目名称及下达编号项目类别项目来源起讫时间科研经费(万元)本人承担任务
教育部新世纪优秀人才支持计划(教计函 [2010]14号) （NCET-09-0926）省部级2010.1-2012.1250.0项目负责人
以蛋白质芳香族氨基酸残基为共振能量转移供体的均相亲和分析方法”，编号**国家级2011.1-2013.1232.0项目负责人
苛求芽孢杆菌尿酸酶的结构与功能关系和分子工程”，编号CSTC 2011 BA 5039省部级2011.7-2.14.720.0项目负责人
“新型免疫检测技术的研究”，科技部863项目 2011AA02A108国家级国际合作研究项目2011.9-2012.12100.0项目负责人
融合表达靶蛋白在微纳米磁珠表面的位点选择性固定化及其应用省部级中央、国家各部门项目2013.1-2015.1212项目负责人