组长：郭惠珊 博士 研究员 博士生导师
研究组研究方向
1、植物RNA沉默的分子机制及其抗病应用研究； 2、植物与真菌的互作机理及维管束真菌病害的防控策略研究
研究组研究内容和意义
本研究组以模式植物拟南芥、烟草和重要经济作物棉花为寄主材料，以病毒和真菌为病原微生物，围绕植物RNA沉默和miRNA调控途径，以及其他受病原影响的调控途径开展病原微生物－寄主的相互作用研究，剖析植物致病机理。为植物抗病和病害防控提供有效策略。
研究组长
郭惠珊 博士 研究员
电话及传真：
电子邮件：guohs#im.ac.cn（请将#换成@）
通讯地址：北京市朝阳区北辰西路1号院3号，
中国科学院微生物研究所
邮编：100101
学习经历
1983.9-1987.7 中山大学生物学，学士
1993.6-1996.6 西班牙马德里大学，博士
工作经历
1987.9-1991.12 中山大学生物工程中心，助教
1992.1-1993.5 西班牙马德里大学"Severo Ochoa"分子生物学中心，Guest Investigator
1997.7-1998.6 西班牙马德里大学国家生物工程中心（CSIC）博士后
1998.7-2003.1 新加坡国立大学分子农业生物学院/淡马锡生命科学研究院Research Fellow
2003.1-2004.6 新加坡国立大学淡马锡生命科学研究院，Acting PI
2004.7-至今 中国科学院微生物所 研究员、博士生导师
获奖情况
2005年 国家****科学基金
2015年 国务院政府特殊津贴
2015年 中国科学院大学岗位教授
2015年 中国科学院特聘骨干人才
2015年 中国植物病理学会会士
2017年 中国科学院特聘核心骨干
学术兼职
中国科学院微生物研究所第十届学术委员会委员
植物基因组学国家重点实验室第四届学术委员会副主任
植物基因组学国家重点实验室第三届学术委员会委员
中国科学院微生物研究所第一届研究组长委员会委员
中国科学院微生物研究所学术道德与权益委员会委员
中国科学院微生物研究所第八届学位评定委员会委员
中国科学院微生物研究所第九届学位评定委员会委员
海南热带生物资源可持续利用重点实验室第一届学术委员会委员
中国植物病理学会第十届理事会理事
生物工程学报 副主编 2017-
Plant Biotechchnology Journal Editorial Board 2018-
MPP Editorial Board 2018-
PBJ Editorial Board 2018-
PloS Pathogens Associate Editor 2014-
MPMI Associate Editor 2013-2015
PloS Pathogens Guest Editor 2013-2014
MPP Associate Editor 2012-2017
BMC Genomics Associate Editor 2014-2018
研究队伍（请将#换成@）
工作人员
赵建华 副研究员 zhao_jian_hua#hotmail.com
金 芸 助理研究员 jiny#im.ac.cn
张 涛高级工程师 zhtlyy#163.com
房媛媛 高级工程师 fangyy#im.ac.cn
在读学生博士后
类别 姓名 性别 入学年份 邮箱
博士 李 洁 女 2013 lijiemuzi2012#163.com
博士 武雪明 女 2016 wuxueming2028#163.com
博士 崔 辰 女 2015 cuichen920218#163.com
博士 吴华伟 男 2016 **#163.com
博士 张博森 男 2019 **@163.com
硕士 尚靖芸 女 262#qq.com
硕士 刘传慧 女 27@qq.com
硕士 刘清艳 女 2019 liuqingyan19@mails.ucas.ac.cn
博士后 王 青 男 2017
已培养学生博士后
类别 姓名 毕业年份
博士 段成国 2008年
博士 杜全生 2008年
博士 何祥凤 2009年
博士 应晓宝 2010年
硕士 李 焱 2010年
博士 朱 慧 2011年
博士 周邦军 2012年
博士 徐 宁 2012年
博士 房媛媛 2013年
博士 冯 镭 2013年
博士 赵 盼 2014年
博士 赵建华 2015年
博士 汪晶晶 2015年
博士 赵云龙 2016年
博士 倪 浩 2016年
博士 张 涛 2016年
博士 周婷婷 2016年
硕士 杜 璇 2017年
博士 陈中祺 2018年
硕士 马晓薇 2018年
博士 刘晓兰 2019年
博士 王 胜 2019年
博士后 杨丽萍 2012年
博士后 梁祥修 2018年
博士后 高 峰 2020年
代表性论文
Cui, C.#, Wang, J.J.#, Zhao, J.H.#, Fang, Y.Y, He, X.F., Guo, H.S.*, Duan, C.G.*(2020). A Brassica miRNA Regulates Plant Growth and Immunity through Distinct Modes of Action. Molecular Plant 13(2): 231-245.
Gao, F.#, Zhang, B.S.#, Zhao, J.H., Huang, J.F., Jia, P.S., Wang, S., Zhang, J., Zhou, J.M., Guo, H.S.*（2019）Deacetylation of chitin oligomers increases virulence in soil-borne fungal pathogens. Nature Plants 5(11): 1167-1176.
Zhao, J. H., Guo, H.S.*（2019）.Trans-kingdom RNA interactions drive the evolutionary arms race between hosts and pathogens. Curr Opin Genet Dev 58-59:62-69.
Liu, X.L., Zhao, J.H., Guo, H.S.*（2019）.IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene. J Genet Genomics 46（3）：149-153.
Jin, Y., Zhao, J. H., Zhao, P., Zhang, T., Wang, S., Guo, H.S.*. （2019）A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae. Philos Trans R Soc Lond B Biol Sci 374（1767）：**.
Zhao, J. H., Liu, X. L., Fang, Y. Y., Fang, R. X., Guo, H.S.*.（2018）CMV2b-Dependent Regulation of Host Defense Pathways in the Context of Viral Infection. Viruses 10（11).
Qin, J., Wang, K., Sun, L., Xing, H., Wang, S., Li, L., Chen, S., Guo, H.S.*, and Zhang, J.* (2018). The plant specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity. eLife 7.
Jin, Y.#, Zhao, P.#, Fang, Y.Y., Gao, F., Guo, H.S.*, and Zhao, J.H.* (2018). Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots. Mycology, 1-11.
Hua,C.L.#, Zhao, J.H.#, and Guo, H.S.* (2018). Trans-kingdom RNA silencing in Plant–Fungal Pathogen Interactions. Mol Plant 11, 235-244.
Du, X., Wang, S., Gao, F., Zhang, L., Zhao, J.H., Guo, H.S.*, and Hua, C.L.* (2017). Expression of pathogenesis-related genes in cotton roots in response to Verticillium dahliae PAMP molecules. Science China. Life sciences 60, 852-860.
Jin, Y., Zhang, T., and Guo, H.S.* (2017). Application of host induced gene silencing in crop protection against fungal diseases. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 33, 161-169.
Zhang, L.S.#, Ni, H.#, Du, X., Wang, S., Ma, X.W., Nurnberger, T., Guo, H.S.*, and Hua, C.L.* (2017). The Verticillium-specific protein VdSCP7 localizes to the plant nucleus and modulates immunity to fungal infections. The New phytologist 215, 368-381.
Zhao, Y.L.*, Zhang, T., and Guo, H.S. (2017). Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae. Bio-Protocol 7.
Zhou, T.T., Zhao, Y.L., and Guo, H.S.* (2017). Secretory proteins are delivered to the septin-organized penetration interface during root infection by Verticillium dahliae. PLoS pathogens 13, e**.
Fang, Y.Y.#, Zhao, J.H.#, Liu, S.W., Wang, S., Duan, C.G., and Guo, H.S.* (2016). CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Frontiers in Microbiology 7, 1329.
Lv, D.Q.#, Liu, S.W.#, Zhao, J.H., Zhou, B.J., Wang, S.P., Guo, H.S., and Fang, Y.Y. * (2016). Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein. Scientific reports 6, 35751.
Wang, S., Xing, H., Hua, C., Guo, H.S.*, and Zhang, J.* (2016). An improved single-step cloning strategy simplifies the Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene disruption method in Verticillium dahliae. Phytopathology.
Zhang, T., Jin, Y., Zhao, J.H., Gao, F., Zhou, B.J., Fang, Y.Y., and Guo, H.S.* (2016). Host-Induced Gene Silencing of the Target Gene in Fungal Cells Confers Effective Resistance to the Cotton Wilt Disease Pathogen Verticillium dahliae. Mol Plant 9, 939-942.
Zhang, T.#, Zhao, Y.L.#, Zhao, J.H.#, Wang, S., Jin, Y., Chen, Z.Q., Fang, Y.Y., Hua, C.L., Ding, S.W., and Guo, H.S.* (2016). Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen. Nature plants 2, 16153.
Zhao, J.H.*, Fang, Y.Y., Duan, C.G., Fang, R.X., Ding, S.W., and Guo, H.S.* (2016). Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis. Scientific reports 6, 36247.
Zhao, J.H., Hua, C.L., Fang, Y.Y., and Guo, H.S.* (2016). The dual edge of RNA silencing suppressors in the virus-host interactions. Current opinion in virology 17, 39-44.
Zhao, Y.L., Zhou, T.T., and Guo, H.S.* (2016). Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+ Signaling Is Required for Plant Infection by Verticillium dahliae. PLoS pathogens 12, e**.
Fang, Y.Y., Smith, N.A., Zhao, J.H., Lee, J.R., Guo, H.S., and Wang, M.B.* (2015). Cloning and profiling of small RNAs from cucumber mosaic virus satellite RNA. Methods in molecular biology 1236, 99-109.
Jin, Y., and Guo, H.S.* (2015). Transgene-induced gene silencing in plants. Methods Mol Biol 105-117.
Shen, W.X., Au, P.C., Shi, B.J., Smith, N.A., Dennis, E.S., Guo, H.S., Zhou, C.Y., and Wang, M.B.* (2015). Satellite RNAs interfere with the function of viral RNA silencing suppressors. Front Plant Sci 281.
Wang, J.J., Zhang, L., and Guo, H.S.* (2015). Arabidopsis Leaf Explant Culture. Bio-Protocol 5(22).
Wang, J.J., and Guo, H.S.* (2015). Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by microRNA847 upregulates auxin signaling to modulate cell proliferation and lateral organ growth in Arabidopsis. The Plant Cell 574-590.
Zahid, K.#, Zhao, J.H.#, Smith, N.A., Schumann, U., Fang, Y.Y., Dennis, E.S., Zhang, R., Guo, H.S.*, and Wang, M.B.* (2015). Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA. PLoS genetics 11, e**.
Zhao, P., Zhao, Y.L., Jin, Y., Zhang, T., and Guo, H.S.* (2014). Colonization process of Arabidopsis thaliana roots by a green fluorescent protein-tagged isolate of Verticillium dahliae. Protein & cell 5, 94-98.
Feng, L.#, Duan, C.G.#, and Guo, H.S.* (2013). Inhibition of in vivo Slicer activity of Argonaute protein 1 by the viral 2b protein independent of its dsRNA-binding function. Molecular plant pathology 14, 617-622.
Yang, L.P., Fang, Y.Y., An, C.P., Dong, L., Zhang, Z.H., Chen, H., Xie, Q., and Guo, H.S.* (2013). C2-mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus. The Plant journal 73, 910-917.
Duan, C.G.#, Fang, Y.Y.#, Zhou, B.J., Zhao, J.H., Hou, W.N., Zhu, H., Ding, S.W., and Guo, H.S.* (2012). Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein. The Plant cell 24, 259-274.
Duan, C.G., Wang, C.H., and Guo, H.S.* (2012). Application of RNA silencing to plant disease resistance. Silence 3, 5.
Xu, N., Li, Y., Zhao, Y.T., Guo, L., Fang, Y.Y., Zhao, J.H., Wang, X.J., Huang, L., and Guo, H.S.* (2012). Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. PloS one 7, e35306.
Zhou, B.J., Jia, P.S., Gao, F., and Guo, H.S.* (2012). Molecular characterization and functional analysis of a necrosis- and ethylene-inducing, protein-encoding gene family from Verticillium dahliae. Molecular plant-microbe interactions : MPMI 25, 964-975.
Zhu, H., and Guo, H.S.* (2012). The role of virus-derived small interfering RNAs in RNA silencing in plants. Science China. Life sciences 55, 119-125.
Dong, L., Liu, M., Fang, Y.Y., Zhao, J.H., He, X.F., Ying, X.B., Zhang, Y.Y., Xie, Q., Chua, N.H., and Guo, H.S.* (2011). DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene. The Plant journal 68, 633-645.
Hou, W.N., Duan, C.G., Fang, R.X., Zhou, X.Y., and Guo, H.S.* (2011). Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection. Molecular plant pathology 12, 595-605.
Zhang, Z., Chen, H., Huang, X., Xia, R., Zhao, Q., Lai, J., Teng, K., Li, Y., Liang, L., Du, Q., Zhou, X., Guo, H., and Xie, Q.* (2011). BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis. The Plant cell 23, 273-288.
Zhu, H., Duan, C.G., Hou, W.N., Du, Q.S., Lv, D.Q., Fang, R.X., and Guo, H.S.* (2011). Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. Journal of virology 85, 13384-13397.
Chen, H., Zhang, Z., Teng, K., Lai, J., Zhang, Y., Huang, Y., Li, Y., Liang, L., Wang, Y., Chu, C., Guo, H., and Xie, Q.* (2010). Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway. The Plant journal 62, 12-23.
Gao, F.#, Zhou, B.J.#, Li, G.Y., Jia, P.S., Li, H., Zhao, Y.L., Zhao, P., Xia, G.X., and Guo, H.S.* (2010). A glutamic acid-rich protein identified in Verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. PloS one 5, e15319.
Li, Y., Liu, X., Huang, L., Guo, H.S.*, and Wang, X.J.* (2010). Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea. Journal of Genetics and Genomics 37, 493-503.
Liu, L.J., Zhang, Y.Y., Tang, S.Y., Zhao, Q.Z., Zhang, Z.H., Zhang, H.W., Dong, L., Guo, H.S., and Xie, Q.* (2010). An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana. The Plant journal 61, 893-903.
Teng, K.L., Chen, H., Lai, J.B., Zhang, Z.H., Fang, Y.Y., Xia, R., Zhou, X.P., Guo, H.S., and Xie, Q.* (2010). Involvement of C4 protein of beet severe curly top virus (family Geminiviridae) in virus movement. PloS one 5, e11280.
Wang, W.Q., Wu, Y.R., Li, Y., Xie, J.Y., Zhang, Z.H., Deng, Z.Y., Zhang, Y.Y., Yang, C.P., Lai, J.B., Zhang, H.W., Bao, H.Y., Tang, S.Y., Yang, C.W., Gao, P., Xia, G.X., Guo, H.S., and Xie, Q. *(2010). A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes. Plant molecular biology 72, 91-99.
Ying, X.B., Dong, L., Zhu, H., Duan, C.G., Du, Q.S., Lv, D.Q., Fang, Y.Y., Garcia, J.A., Fang, R.X., and Guo, H.S.* (2010). RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. The Plant cell 22, 1358-1372.
Lai, J.B., Chen, H., Teng, K.L., Zhao, Q.Z., Zhang, Z.H., Li, Y., Liang, L., Xia, R., Wu, Y.R., Guo, H.S., and Xie, Q.* (2009). RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. The Plant journal 57, 905-917.
Zheng, N.Y., Xia, R., Yang, C.W., Yin, B., Li, Y., Duan, C.G., Liang, L., Guo, H.S., and Xie, Q.*(2009). Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. Vaccine 27, 5001-5007.
Duan, C.G., Wang, C.H., Fang, R.X., and Guo, H.S.* (2008). Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. Journal of virology 82, 11084-11095.
He, X.F., Fang, Y.Y., Feng, L., and Guo, H.S.* (2008). Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR–NBS–LRR class R gene-derived novel miRNA in Brassica. FEBS letters 582, 2445-2452.
Zhang, Y.Y., Li, Y., Gao, T., Zhu, H., Wang, D.J., Zhang, H.W., Ning, Y.S., Liu, L.J., Wu, Y.R., Chu, C.C., Guo, H.S., and Xie, Q.* (2008). Arabidopsis SDIR1 enhances drought tolerance in crop plants. Bioscience, biotechnology, and biochemistry 72, 2251-2254.
Du, Q.S.#, Duan, C.G.#, Zhang, Z.H.#, Fang, Y.Y., Fang, R.X., Xie, Q., and Guo, H.S.* (2007). DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. Journal of virology 81, 9142-9151.
Zhang, Y.Y., Yang, C.W., Li, Y., Zheng, N.Y., Chen, H., Zhao, Q.Z., Gao, T., Guo, H.S., and Xie, Q.* (2007). SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis. The Plant cell 19, 1912-1929.
Zhang, Z.H., Xie, Q., and Guo, H.S.* (2007). Antiviral defense in plants. Plant Viruses 1, 21-26.
Xie, Q., and Guo, H.S.* (2006). Systemic antiviral silencing in plants. Virus research 118, 1-6.
Guo, H.S., Xie, Q., Fei, J.F., and Chua, N.H*. (2005). MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development. The Plant cell 17, 1376-1386.
代表性图片
miR1885动态调控油菜的生长发育与免疫。（Molecular Plant 2020）
乙酰基团（acetyl）在几丁质寡糖被膜受体识别诱发免疫反应中起关键作用；VdPDA1去乙酰化抑制了几丁质寡糖诱导的膜受体的二聚化，从而抑制诱导免疫反应。（Nature plants 2019）
大丽轮枝菌侵染棉花诱导内源miRNA的积累；从感病棉花分离得到的菌丝中检测到植物miRNA，并下调真菌致病基因的积累。（Nature plants 2016）
