文献详情
A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector
文献类型：期刊
通讯作者：Long, F (reprint author), Renmin Univ China, Sch Environm & Nat Resources, Beijing 100872, Peoples R China.; Zhu, AN (reprint author), Acad Mil Sci PLA China, Res Inst Chem Def, Beijing 102205, Peoples R China.
期刊名称：TALANTA影响因子和分区
年：2019
卷：196
页码：78-84
ISSN：0039-9140
关键词：Dual-color fluorescence; Total internal reflection; Fiber optic probe; Evanescent wave; Fiber optic switch
所属部门：环境学院
摘要：A novel dual-color total internal reflection fluorescence detecting platform (DTP) was developed for the simultaneous detection of two fluorescence dyes. The DTP employed a simple and compact optical system and a Si-based photodetector SOP-1000 assembly to improve the optical efficiency and detection sensitivity. In the optical system, a single-multimode fiber optic coupler was applied for the transmission of two wavelength excitation lights and the collection and transmission of two wavelength ...More
A novel dual-color total internal reflection fluorescence detecting platform (DTP) was developed for the simultaneous detection of two fluorescence dyes. The DTP employed a simple and compact optical system and a Si-based photodetector SOP-1000 assembly to improve the optical efficiency and detection sensitivity. In the optical system, a single-multimode fiber optic coupler was applied for the transmission of two wavelength excitation lights and the collection and transmission of two wavelength fluorescence signals, and a fiber optical switch controlled two wavelength excited lights to alternatively enter into the combined tapered fiber optic probe and two wavelength fluorescence dyes were alternatively excited. The simultaneous and sensitive detection of two wavelength fluorescence signals was achieved by one photodetector SOP-1000 based on the time resolved effect of the fiber optical switch. The DTP demonstrated its sensitivity of 50 fW light intensity, and the limit of detection of Cy5.5 and Pacific Blue dye (PB) were 0.05 nM and 2.1 nM, respectively. This miniaturized and integrated DTP with high sensitivity, simple optical structure, negligible cross-talk, and cost-effectiveness could be a potential alternative to the conventional dual-color fluorescence detecting systems. It could also be a powerful component of a mu-TAS system for highly sensitive dual-color fluorescence detection. ...Hide

DOI：10.1016/j.talanta.2018.12.039
百度学术：A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector
语言：外文
人气指数：3
浏览次数：3
基金：National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21675171, 21277173]; National Instrument Major Project of China [2012YQ3011105]; Special Fund of State Key Joint Laboratory of Environment Simulation and Pollution Control [17K06ESPCT]
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