文献详情
CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance
文献类型：期刊
通讯作者：Shen, L (reprint author), China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China.
期刊名称：BMC PLANT BIOLOGY影响因子和分区
年：2019
卷：19
ISSN：1471-2229
关键词：CRISPR; Cas9; SlNPR1; Drought; ROS; Stomatal closure; Tomato plant
所属部门：农业与农村发展学院
摘要：BackgroundNPR1, nonexpressor of pathogenesis-related gene 1, is a master regulator involved in plant defense response to pathogens, and its regulatory mechanism in the defense pathway has been relatively clear. However, information about the function of NPR1 in plant response to abiotic stress is still limited. Tomato is the fourth most economically crop worldwide and also one of the best-characterized model plants employed in genetic studies. Because of the lack of a stable tomato NPR1 (SlNPR1) ...More
BackgroundNPR1, nonexpressor of pathogenesis-related gene 1, is a master regulator involved in plant defense response to pathogens, and its regulatory mechanism in the defense pathway has been relatively clear. However, information about the function of NPR1 in plant response to abiotic stress is still limited. Tomato is the fourth most economically crop worldwide and also one of the best-characterized model plants employed in genetic studies. Because of the lack of a stable tomato NPR1 (SlNPR1) mutant, little is known about the function of SlNPR1 in tomato response to biotic and abiotic stresses.ResultsHere we isolated SlNPR1 from tomato Ailsa Craig' and generated slnpr1 mutants using the CRISPR/Cas9 system. Analysis of the cis-acting elements indicated that SlNPR1 might be involved in tomato plant response to drought stress. Expression pattern analysis showed that SlNPR1 was expressed in all plant tissues, and it was strongly induced by drought stress. Thus, we investigated the function of SlNPR1 in tomato-plant drought tolerance. Results showed that slnpr1 mutants exhibited reduced drought tolerance with increased stomatal aperture, higher electrolytic leakage, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels, and lower activity levels of antioxidant enzymes, compared to wild type (WT) plants. The reduced drought tolerance of slnpr1 mutants was further reflected by the down-regulated expression of drought related key genes, including SlGST, SlDHN, and SlDREB.ConclusionsCollectively, the data suggest that SlNPR1 is involved in regulating tomato plant drought response. These results aid in further understanding the molecular basis underlying SlNPR1 mediation of tomato drought sensitivity. ...Hide

DOI：10.1186/s12870-018-1627-4
百度学术：CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance
语言：外文
被引频次：7
人气指数：4
浏览次数：4
基金：National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31371847, 31571893]
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