窦欣^1,,
田乔鹏¹,
王琦¹,
管政兵¹,
蔡宇杰¹,
廖祥儒^1,
1.江南大学生物工程学院，工业生物技术教育部重点实验室，无锡 214122
基金项目: 江苏省产学研前瞻项目BY2014023-28江苏省产学研前瞻项目(BY2014023-28)

Characterization of laccase from Ganoderma sp. SYBC L48 and its performance on acid red one decolorization

DOU Xin^1,,
TIAN Qiaopeng¹,
WANG Qi¹,
GUAN Zhengbing¹,
CAI Yujie¹,
LIAO Xiangru^1,
1.Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

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摘要:对灵芝菌Ganoderma sp. SYBC L48漆酶进行了纯化和酶学性质分析，并利用该漆酶对偶氮染料酸性红1进行脱色处理；考察了脱色体系中各因素对脱色效率的影响；采用小麦种子和水稻种子对酶处理后的染料进行了毒性测试。结果表明，以ABTS为底物时，该酶的最适pH为2.5，最适温度为60 ℃，在pH 5~9和20~60 ℃具有良好的稳定性，Co2+、Cr3+和Fe3+离子对酶活性有较强的抑制作用。在染料浓度100 mg·L-1，酶浓度0.5 U·mL-1，介体HOBT浓度0.25 mmol·L-1，pH为4，50 ℃的条件下反应30 min后，该漆酶对酸性红1的脱色率可达90.3%；1 mmol·L-1的Cr3+、Cu2+、Al3+和Ni2+存在下，漆酶仍能催化酸性红1脱色；脱色后染料的植物毒性下降。上述结果表明该漆酶在纺织废水处理中具有一定的应用前景。
关键词: 灵芝漆酶/
酶学性质/
酶脱色/
偶氮染料废水治理/
酸性红1

Abstract:In this study, the laccase from Ganoderma sp. SYBC L48 was purified and its enzymatic properties were analyzed, then this purified laccase was used to decolorize the azo dye acid red one. The factors affecting dye decolorization rate in the reaction system were investigated, and the toxicity of enzyme treated dyes was evaluated with wheat and rice seeds. The results showed that the best activity of the purified laccase appeared at pH 2.5 and 60 ℃ with ABTS as the substrate, and an excellent stable enzyme activity occurred at pH 5~9 and 20~60 ℃, respectively. However, Co2+, Al3+ and Fe3+ ions had strong inhibition effects on the enzyme activity. The dye docolorization efficiency reached 90.3% after 30 min enzyme treating 100 mg·L-1 azo dye acid red one solution at 0.5 U·mL-1 laccase, 0.25 mmol·L-1 mediator of HOBT, pH 4 and 50 ℃. Even under the existence of 1 mmol·L-1 Cr3+, Cu2+, Al3+ and Ni2+, the purified laccase could decolorize acid red one. In addition, the plant toxicity of decolorized dye decreased. The above results indicated that the purified laccase has a certain application prospect in textile wastewater treatment.
Key words:Ganoderma laccase/
enzymatic properties/
enzyme decolorization/
azo dye decolorization/
acid red one.

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Ganoderma sp. SYBC L48漆酶酶学性质及其对酸性红1的脱色性能

窦欣^1,,
田乔鹏¹,
王琦¹,
管政兵¹,
蔡宇杰¹,
廖祥儒^1,
1.江南大学生物工程学院，工业生物技术教育部重点实验室，无锡 214122
基金项目: 江苏省产学研前瞻项目BY2014023-28江苏省产学研前瞻项目(BY2014023-28)
关键词: 灵芝漆酶/
酶学性质/
酶脱色/
偶氮染料废水治理/
酸性红1
摘要:对灵芝菌Ganoderma sp. SYBC L48漆酶进行了纯化和酶学性质分析，并利用该漆酶对偶氮染料酸性红1进行脱色处理；考察了脱色体系中各因素对脱色效率的影响；采用小麦种子和水稻种子对酶处理后的染料进行了毒性测试。结果表明，以ABTS为底物时，该酶的最适pH为2.5，最适温度为60 ℃，在pH 5~9和20~60 ℃具有良好的稳定性，Co2+、Cr3+和Fe3+离子对酶活性有较强的抑制作用。在染料浓度100 mg·L-1，酶浓度0.5 U·mL-1，介体HOBT浓度0.25 mmol·L-1，pH为4，50 ℃的条件下反应30 min后，该漆酶对酸性红1的脱色率可达90.3%；1 mmol·L-1的Cr3+、Cu2+、Al3+和Ni2+存在下，漆酶仍能催化酸性红1脱色；脱色后染料的植物毒性下降。上述结果表明该漆酶在纺织废水处理中具有一定的应用前景。

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