王东升^1,,
王立立¹,
李取生¹,
周婷¹,
周雪芳¹,
高琼¹
1.暨南大学环境学院，广东省环境污染与健康重点实验室，广州510632
基金项目: 国家重点研发计划项目（2017YFD0801305）
广东省科技计划项目（2017A010105005）

Enhancing effect of siderophore-producting bacteria on remediation of cadmium-contaminated soil by Solanum nigrum L

WANG Dongsheng^1,,
WANG Lili¹,
LI Qusheng¹,
ZHOU Ting¹,
ZHOU Xuefang¹,
GAO Qiong¹
1.Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China

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摘要:通过CAS筛选培养基从龙葵根系土中筛选出2株产铁载体菌T1、Y2，经生理生化鉴定和16S rDNA序列分析，初步确定T1、Y2分别为铜绿假单胞菌和阴沟肠杆菌。将2株细菌接种至溶镉溶磷培养基中进行实验室培养，并对溶镉、溶磷结果和2株菌的胞外分泌物进行分析。结果表明,接种T1、Y2菌培养基中的有效镉、有效磷与不接菌对照相比显著增加（P0.05），接种T1、Y2菌处理的转运系数与未接菌对照相比未显著性变化（P>0.05）。接种菌处理有效促进了龙葵根系土壤难溶性形态Cd向可交换态Cd的转化。同时，研究发现接种T1、Y2菌可显著促进（P<0.05）龙葵对土壤Cd的吸收，强化了龙葵修复土壤镉污染的能力。
关键词: 产铁载体菌/
龙葵/
胞外分泌物/
Cd污染/
土壤修复

Abstract:Two strains of siderophore-producting bacteria T1 and Y2 were isolated from the root system soil of Solanum nigrum L by CAS screening medium. Physiological and biochemical identification and 16S rDNA sequence analysis confirmed that T1 and Y2 were Pseudomonas aeruginosa and Enterobacter cloacae, respectively. These strains were inoculated into the cadmium and phosphorus-dissolving medium for the determination of cadmium and phosphate dissolution, and the analysis of extracellular secretions. The results showed that the effective cadmium and available phosphorus in the culture medium inoculated with T1 and Y2 were significantly higher than those in the non-inoculated control (P0.05). The transport coefficient of Solanum nigrum L treated with T1 and Y2 inoculation did not change significantly compared with the control (P>0.05). The transformation of insoluble Cd to exchangeable form in root soil of Solanum nigrum L was promoted. Meanwhile it was found that inoculation with strains T1 and Y2 could significantly promote the uptake of Cd (P<0.05) and enhance the ability of Solanum nigrum L to remediate polluted soil with cadmium.
Key words:siderophore-producting bacteria/
Solanum nigrum L/
extracellular secretion/
Cd-contaminated/
soil remediation.

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刊出日期:2018-08-17

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产铁载体菌对龙葵修复土壤Cd污染的促进效应

王东升^1,,
王立立¹,
李取生¹,
周婷¹,
周雪芳¹,
高琼¹
1.暨南大学环境学院，广东省环境污染与健康重点实验室，广州510632
基金项目: 国家重点研发计划项目（2017YFD0801305） 广东省科技计划项目（2017A010105005）
关键词: 产铁载体菌/
龙葵/
胞外分泌物/
Cd污染/
土壤修复
摘要:通过CAS筛选培养基从龙葵根系土中筛选出2株产铁载体菌T1、Y2，经生理生化鉴定和16S rDNA序列分析，初步确定T1、Y2分别为铜绿假单胞菌和阴沟肠杆菌。将2株细菌接种至溶镉溶磷培养基中进行实验室培养，并对溶镉、溶磷结果和2株菌的胞外分泌物进行分析。结果表明,接种T1、Y2菌培养基中的有效镉、有效磷与不接菌对照相比显著增加（P0.05），接种T1、Y2菌处理的转运系数与未接菌对照相比未显著性变化（P>0.05）。接种菌处理有效促进了龙葵根系土壤难溶性形态Cd向可交换态Cd的转化。同时，研究发现接种T1、Y2菌可显著促进（P<0.05）龙葵对土壤Cd的吸收，强化了龙葵修复土壤镉污染的能力。

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