中文关键词石化废水耐药菌(ARB)耐药基因(ARGs)分布去除影响因子 英文关键词petrochemical wastewaterantibiotic-resistant bacteria(ARB)antibiotic resistance genes(ARGs)distributionremovalinfluencing factor

作者	单位	E-mail
唐振平	南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001 南华大学稀有金属矿产开发与废物地质处置技术湖南省重点实验室, 衡阳 421001	zptang01@126.com
肖莎莎	南华大学土木工程学院, 衡阳 421001
段毅	南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001 南华大学土木工程学院, 衡阳 421001
刘迎九	南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001 南华大学土木工程学院, 衡阳 421001
高媛媛	南华大学土木工程学院, 衡阳 421001
吴月月	南华大学衡阳医学院病原生物学研究所, 衡阳 421001
陈怡雯	南华大学衡阳医学院病原生物学研究所, 衡阳 421001
周帅	南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001 南华大学土木工程学院, 衡阳 421001	zs402606665@126.com

中文摘要 抗生素耐药性污染已成为全球新兴环境问题之一.本研究选取某座石化废水处理厂，对耐药菌（ARB）和3种形态耐药基因（ARGs）：细胞内耐药基因（iARGs）、细胞外附着态耐药基因（aeARGs）和游离态耐药基因（feARGs）的分布特征与去除效能开展研究.结果表明，废水处理厂中检出四环素、磺胺和氨苄西林这3类ARB，其绝对浓度为8.45×10²~2.38×10⁵ CFU·mL^-1.厌氧处理可使这3类ARB绝对浓度下降0.04 lg~0.21 lg；曝气和沉淀处理对ARB的影响因其类型而异；出水ARB绝对浓度高出进水水平0.12 lg~0.63 lg.活性污泥中aeARGs和iARGs绝对丰度分别为1.96×10⁷~3.02×10¹⁰ copies·g^-1和5.22×10⁷~4.15×10¹⁰ copies·g^-1；而废水中feARGs绝对丰度为5.90×10⁸~1.01×10¹² copies·L^-1.厌氧处理可去除0.13 lg~0.65 lg aeARGs和0.04 lg~0.28 lg iARGs；曝气和沉淀处理对aeARGs和iARGs的去除效果受ARGs类型和形态影响；出水中feARGs绝对丰度较进水升高0.06 lg~0.81 lg.冗余分析表明，ARB浓度与COD、Cl^-和总氮浓度显著正相关（P<0.05）；aeARGs丰度与COD和总氮浓度显著正相关（P<0.05）；iARGs和feARGs丰度均与重金属浓度显著正相关（P<0.05）.本研究证实了石化废水处理厂具有ARB和不同形态ARGs的富集风险，并为特种工业废水耐药性污染研究与防治提供理论基础. 英文摘要 A petrochemical wastewater treatment plant (PWWTP) was selected to investigate the distribution and removal of antibiotic-resistant bacteria (ARB) and three forms of antibiotic resistance genes (ARGs), namely intracellular ARGs (iARGs), adsorbed-extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs). Tetracycline, sulfanilamide, and ampicillin ARB were detected with the total absolute concentration of 8.45×10²-2.38×10⁵ CFU·mL^-1; the absolute concentrations of three types of ARB decreased by 0.04 lg-0.21 lg through anaerobic treatment. The effect of aeration and precipitation treatment on ARB varies with its type, and the absolute concentration of ARB in effluent was 0.12 lg-0.63 lg higher than that in influent. The absolute abundance of aeARGs and iARGs in activated sludge was 1.96×10⁷-3.02×10¹⁰ copies·g^-1 and 5.22×10⁷-4.15×10¹⁰ copies·g^-1, respectively; the absolute abundance of feARGs in wastewater was 5.90×10⁸-1.01×10¹² copies·L^-1. Anaerobic treatment can remove 0.13 lg-0.65 lg aeARGs and 0.04 lg-0.28 lg iARGs, while the removal efficiency of aeARGs and iARGs by aeration and precipitation process was affected by ARGs types and forms. The absolute abundance of feARGs in effluent is 0.06 lg-0.81 lg higher than that in influent. Redundancy analysis showed that the concentration of ARB was significantly positively correlated with chemical oxygen demand (COD), Cl^-, and total nitrogen concentration (P<0.05). The abundance of aeARGs was positively correlated with COD and total nitrogen concentration (P<0.05), and both the abundance of iARGs and feARGs are positively correlated with heavy metals concentration (P<0.05). This study confirmed the enrichment risk of ARB and different forms of ARGs in PWWTPs, which provided references for the research and prevention of antibiotic resistance pollution in industrial wastewater.

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