中文关键词抗生素抗性基因(ARGs)宏基因组学渔业复垦微生物群落沉积物井水 英文关键词antibiotic resistance genes (ARGs)metagenomicsfishery reclamationmicrobial communitysedimentwell water

作者	单位	E-mail
程森	中国矿业大学环境与测绘学院, 徐州 221000	chengsen9711@163.com
路平	中国矿业大学环境与测绘学院, 徐州 221000	lupingcumt@126.com
冯启言	中国矿业大学低碳研究院, 徐州 221000

中文摘要 水产养殖行业抗生素的广泛使用引起了抗生素抗性基因（antibiotic resistance genes，ARGs）污染问题.为探究渔业复垦塌陷地ARGs污染特征，利用宏基因组学技术检测分析了渔业复垦塌陷地ARGs的相对丰度和微生物群落结构.研究区域共检测出29种ARGs，bacA在所有样品中相对丰度最高，达1.96×10^-5~1.19×10^-4.沉积物中磺胺类和四环素类ARGs相对丰度较高，井水中多药类ARGs相对丰度较高.微生物群落结构表明变形菌门（Proteobacteria）在所有样品中为最优势细菌门，沉积物中绿弯菌门（Chloroflexi）和广古菌门（Euryarchaeota）较为优势.属水平上，硫杆菌属（Thiobacillus）为沉积物中最优势细菌属，不动杆菌属（Acinetobacter）和假单胞菌属（Pseudomonas）为井水中优势细菌属.ARGs与微生物相关性分析表明，菌属与ARGs间主要呈中度相关，多种菌属与ARGs显著正相关，ARGs的分布受微生物群落结构的重要影响.渔业复垦塌陷地沉积物与井水均受到ARGs污染，应加强相应控制措施保护区域环境. 英文摘要 The widespread use of antibiotics in the aquaculture industry has caused antibiotic resistance genes (ARGs) pollution. Metagenomics technology was used to detect and analyze the relative abundance of ARGs and microbial community structure in a fishery reclamation mining subsidence area. A total of 29 ARGs were detected, and bacA had the highest relative abundance in all the samples, reaching 1.96×10^-5-1.19×10^-4. The relative abundance of sulfonamide and tetracycline ARGs in sediments was relatively high and the relative abundance of multidrug ARGs in well water was relatively high. Proteobacteria was the most dominant bacterial phylum in all the samples, and Chloroflexi and Euryarchaeota were relatively abundant in the sediments. Thiobacillus was the most dominant bacterial genus in the sediments, and Acinetobacter and Pseudomonas were the dominant bacterial genera in the well water. The correlation analysis between the ARGs and microorganisms showed that the genera and ARGs were mainly correlated to a moderate degree, and multiple genera had significant positive correlations with ARGs. The distribution of ARGs was affected by the structure of the microbial community. The sediments and well water in the fishery reclamation mining subsidence area were both contaminated by ARGs, and corresponding control measures should be strengthened to protect the regional environment.

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