中文关键词聚醚砜(PES)2,4-二氯苯酚(2,4-DCP)厌氧颗粒污泥胞外聚合物(EPS)微生物群落 英文关键词polyether sulfone (PES)2,4-dichlorophenol (2,4-DCP)anaerobic granular sludgeextracellular polymeric substances (EPS)microbial community

作者	单位	E-mail
林旭萌	广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室, 桂林 541004 广西师范大学环境与资源学院, 桂林 541004	474325174@qq.com
宿程远	广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室, 桂林 541004 广西师范大学环境与资源学院, 桂林 541004	suchengyuan2008@126.com
吴淑敏	广西师范大学环境与资源学院, 桂林 541004
黄娴	广西师范大学环境与资源学院, 桂林 541004
邓雪	广西师范大学环境与资源学院, 桂林 541004
林香凤	广西师范大学环境与资源学院, 桂林 541004
黄尊	广西师范大学环境与资源学院, 桂林 541004
魏佳林	广西师范大学环境与资源学院, 桂林 541004

中文摘要 考察了聚醚砜（PES）微塑料及2，4-二氯苯酚（2，4-DCP）对厌氧颗粒污泥疏松胞外聚合物（LB-EPS）和紧密胞外聚合物（TB-EPS）组分的影响，并利用高通量测序技术对厌氧颗粒污泥的微生物群落及基因功能变化进行了分析.结果表明，2，4-DCP以及PES+2，4-DCP实验组COD去除率分别为35%和37%，与空白对照组相比降低了57%和55%；而PES实验组COD去除率仍在90%左右.投加PES+2，4-DCP后，厌氧颗粒污泥LB-EPS中的蛋白及多糖含量与对照组相比出现了降低，TB-EPS中多糖含量增加最少.无论投加PES还是2，4-DCP均会抑制辅酶F₄₂₀的活性.通过高通量测序发现投加了PES或2，4-DCP实验组厌氧颗粒污泥的微生物丰度及多样性均减少.在对照组和实验组中，门水平下优势菌群为Proteobacteria（13.45%~44.47%）、Firmicutes（6.86%~21.67%）和Actinobacteria（3.16%~18.11%）；纲水平下PES+2，4-DCP实验组中β-Proteobacteria含量与对照组相比减少了15.28%，γ-Proteobacteria含量与对照组相比增加了28.44%.基于PICRUSt分析发现PES或2，4-DCP实验组中，污泥中能量代谢功能相关基因比对照组增多了0.25%~0.72%；而2，4-DCP实验组污泥中膜运输功能组相关基因丰度减少明显. 英文摘要 The effects of polyether sulfone (PES) microplastics and 2,4-dichlorophenol (2,4-DCP) on the loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound EPS (TB-EPS) of anaerobic granular sludge were investigated. In addition, high-throughput sequencing technology was used to analyze the changes in the microbial community and gene functions in the anaerobic granular sludge. The results revealed that the chemical oxygen demand (COD) removal rates of the 2,4-DCP and PES+2,4-DCP experimental groups were 35% and 37%, which were 57% and 55% lower than that of the blank control group, while the COD removal rates of the PES experimental group remained around 90%. After the addition of the PES microplastics and 2,4-DCP, the protein and polysaccharide contents in the LB-EPS decreased compared with the control group, and the polysaccharide content in TB-EPS increased the least. In presence of the PES microplastics and 2,4-DCP, the activity of coenzyme F₄₂₀ was inhibited. Through high-throughput sequencing, the microbial richness and diversity of the anaerobic granular sludge in the experimental group were reduced with the addition of the PES microplastics or 2,4-DCP. In the control group and the experimental group, the dominant bacteria at the phylum level were Proteobacteria (13.45%-44.47%), Firmicutes (6.86%-21.67%), and Actinobacteria (3.16%-18.11%). The abundance of β-Proteobacteria in the PES+2,4-DCP experimental group was reduced by 15.28%, while the abundance of γ-Proteobacteria increased by 28.44% compared with the control group. Based on the phylogenetic investigation of the communities using the reconstruction of unobserved states (PICRUSt) analysis, it was found that in the experimental group with the PES microplastics or 2,4-DCP, the genes related to the sludge energy metabolism function were 0.25%-0.72% more than the control group; therefore, the abundance of genes related to the transport function group decreased significantly.

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