中文关键词序批式生物反应器(SBR)提盐速率短程硝化胞外聚合物微生物群落结构 英文关键词sequencing batch reactor(SBR)salinity promotion rateshortcut nitrificationextracellular polymeric substancesmicrobial community structure

作者	单位	E-mail
古柏铭	中国海洋大学环境科学与工程学院, 青岛 266100	gbm19970311@163.com
金春姬	中国海洋大学环境科学与工程学院, 青岛 266100 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100	jinhou@ouc.edu.cn
温淳	中国海洋大学环境科学与工程学院, 青岛 266100
侯金源	中国海洋大学环境科学与工程学院, 青岛 266100
赵阳国	中国海洋大学环境科学与工程学院, 青岛 266100 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100
高孟春	中国海洋大学环境科学与工程学院, 青岛 266100 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100

中文摘要 使用序批式生物反应器驯化耐盐活性污泥，探究提盐速率对污染物去除效果、活性污泥特性和微生物群落结构的影响.结果表明，快速提升盐度至30‰（30 d内提升盐度），COD和NH₄⁺-N去除率均出现明显下降，由最初的85.5%和98.5%，分别降低至72.2%和81.7%；缓慢提升盐度至30‰（90 d内提升盐度），COD和NH₄⁺-N去除率受盐度影响较小，表现出良好的去除性能.在缓慢提升盐度过程中，盐度为20‰，出水NO₂^--N升高至11.13 mg·L^-1，NO₃^--N降低为0.56 mg·L^-1，实现短程硝化；盐度为30‰，亚硝酸盐积累率约为90%，TN去除率升高至75%左右.随着盐度升高，胞外聚合物中多糖和蛋白质的含量不断增加，当盐度高于15‰时，多糖明显增加.高通量测序结果表明，经快速提盐和缓慢提盐驯化，微生物多样性明显下降，Shannon指数由8.06分别降为4.34和6.17.随着盐度增加，Micropruina、Denitromonas、TM7a和Marinicella表现出良好的耐盐特性.经快速提盐驯化后，Denitratisoma、Defluviimonas、Arenimonas和Denitromonas等反硝化菌的相对丰度明显减少. 英文摘要 In this study, three sequence batch reactors were selected to evaluate the effects of salt-tolerant activated sludge acclimation. The effect of salinity increase rate on pollutant removal, physicochemical characteristics of activated sludge, and microbial community were investigated. The results showed that a rapid salinity increase to 30‰ (within 30 d) reduced removal efficiencies of COD and NH₄⁺-N from 85.5% and 98.5% (18 d) to 72.2% and 81.7% (51 d), respectively. In comparison, a slower salinity increases to 30‰ (within 90 d) had a minor effect on COD and NH₄⁺-N removal. During the rapid salinity increase, a stable shortcut nitrification occurred under 20‰ salinity, in which the effluent NO₂^--N reached 11.13 mg·L^-1 and NO₃^--N decreased to 0.56 mg·L^-1. When salinity increased to 30‰, the nitrite accumulation rate was about 90%, and the removal efficiency of total nitrogen increased to approximately 75%. The contents of polysaccharide and protein in extracellular polymer substances increased as salinity increased, and the polysaccharide content increased significantly when the salinity was higher than 15‰. High-throughput sequencing results illustrated that microbial diversity reduced as salinity increased, following the Shannon index decrease from 8.06 (0‰ salinity) to 4.34 (rapid salinity increase) and 6.17 (slower salinity increase). As salinity increased, Micropruina, Denitromonas, TM7a, and Marinicella exhibited good salt tolerance. The relative abundance of Denitratisoma, Defluviimonas, Arenimonas, and Denitromonas decreased more significantly following the rapid salinity increase compared with that after the slower salinity increase.

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